Cross‐species transmission of feline herpesvirus 1 (FHV‐1) to chinchillas

Shi, Longyan; Huang, Shuping; Lu, Yuxin; Su, Yuqing; Guo, Lin; Guo, Linlin; Xie, Wenhui; Li, Xiang; Wang, Yulong; Yang, Siyuan; Chai, Hongliang; Wang, Yajun

doi:10.1002/vms3.914

Cited by 2 publications

(1 citation statement)

References 34 publications

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“…Domestic cats act as primary host for FHV-1. However, FHV-1 natural infections have also been reported in several wild animals including leopards, cheetahs, jaguars, and margays [ 15 – 17 ]. FHV-1 transmission occurs through natural routes of nose, mouth, and conjunctiva.…”

Section: Introductionmentioning

confidence: 99%

First report of molecular epidemiology and phylogenetic characteristics of feline herpesvirus (FHV-1) from naturally infected cats in Kunshan, China

Kim,

Cheng,

Fang

et al. 2024

Virol J

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Background Feline herpesvirus type 1 (FHV-1) is a life threatening highly contagious virus in cats and typically causes upper respiratory tract infections as well as conjunctival and corneal ulcers. Genetic variability could alter the severity of diseases and clinical signs. Despite regular vaccine practices against FHV-1 in China, new FHV-1 cases still commonly occur. The genetic and phylogenetic characteristics of FHV-1 in Kunshan city of China has not been studied yet. Therefore, this study was planned to investigate the prevalence, molecular characteristics of circulating strains, and phylogenetic analyses of FHV-1. This is the first report of molecular epidemiology and phylogenetic characteristics of FHV-1 from naturally infected cats in Kunshan, China. Methods The occulo-nasal swabs were collected from diseased cats showing respiratory distress, conjunctivitis, and corneal ulcers at different veterinary clinics in Kunshan from 2022 to 2023. Clinical data and general information were recorded. Swab samples were processed for preliminary detection of FHV-1. Thymidine kinase (TK), glycoprotein B (gB) and glycoprotein D (gD) genes were sequenced and analyzed to investigate genetic diversity and evolution of FHV-1. Results The FHV-1 genome was detected in 43 (43/200, 21.5%) samples using RT-PCR targeting the TK gene. Statistical analysis showed a significant correlation between age, vaccination status and living environment (p < 0.05) with FHV-1 positivity, while a non-significant correlation was observed for FHV-1 positivity and sex of cats (p > 0.05). Additionally, eight FHV-1 positive cats were co-infected with feline calicivirus (8/43,18.6%). FHV-1 identified in the present study was confirmed as FHV-1 based on phylogenetic analyses. The sequence analyses revealed that 43 FHV-1 strains identified in the present study did not differ much with reference strains within China and worldwide. A nucleotide homology of 99-100% was determined among gB, TK and gD genes nucleotide sequences when compared with standard strain C-27 and vaccine strains. Amino acid analysis showed some amino acid substitutions in TK, gB and gD protein sequences. A potential N-linked glycosylation site was observed in all TK protein sequences. Phylogenetic analyses revealed minor variations and short evolutionary distance among FHV-1 strains detected in this study. Conclusions Our findings indicate that genomes of 43 FHV-1 strains are highly homogenous and antigenically similar, and the degree of variation in major envelope proteins between strains is low. This study demonstrated some useful data about prevalence, genetic characteristics, and evolution of FHV-1 in Kunshan, which may aid in future vaccine development.

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Section: Introductionmentioning

confidence: 99%

First report of molecular epidemiology and phylogenetic characteristics of feline herpesvirus (FHV-1) from naturally infected cats in Kunshan, China

Kim,

Cheng,

Fang

et al. 2024

Virol J

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Cross‐species transmission of feline herpesvirus 1 (FHV‐1) to chinchillas

Shi

Huang

et al. 2022

Veterinary Medicine & Sci

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Background: Herpesviruses are a class of double-stranded DNA viruses found in both vertebrates and invertebrates. They are usually highly host-specific and do not easily spread across species. Chinchillas have gradually entered the Chinese pet market in recent years, but references to viral infections in chinchillas are extremely scarce, and only two reports about the herpesvirus in chinchillas are available at present. Objectives:The aim of this study was to present the first report of FHV-1 infection in chinchillas.Methods: A total of 130 nasopharyngeal swab samples of chinchillas and three nasopharyngeal swabs of domestic cats collected from a chinchillas farm were investigated by nested PCR for FHV-1.Results: Four chinchillas were infected with FHV-1, the positive rate was 3.08% (4/130), and two domestic cats were FHV-1 positive (2/3). The 253 bp fragments of FHV-1 gD gene from four chinchillas and two domestic cats were 100% identical, respectively, and the homology between chinchillas and domestic cat was 99.21%, but they all shared nearly 98.81% homology with the reference strain sequences. Phylogenetic tree analysis showed that these four chinchillas strains were clustered together with FHV-1.Conclusions: This is the first time that FHV-1 was detected in chinchillas and suggested chinchillas are susceptible to FHV-1 and may play a role as a temporary reservoir for FHV-1.

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Cross‐species transmission of feline herpesvirus 1 (FHV‐1) to chinchillas

Cited by 2 publications

References 34 publications

First report of molecular epidemiology and phylogenetic characteristics of feline herpesvirus (FHV-1) from naturally infected cats in Kunshan, China

First report of molecular epidemiology and phylogenetic characteristics of feline herpesvirus (FHV-1) from naturally infected cats in Kunshan, China

Cross‐species transmission of feline herpesvirus 1 (FHV‐1) to chinchillas

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