Cross‐reactivity of some antibodies to human epitopes with shrimp <i>Pandalus borealis</i> proteins: a possible aid in validation and characterization of crustacean cells <i>in vitro</i>

Lyons-Alcantara, Maria; Lambkin, Helen; Nordmo, R.; Lyng, Fiona M.; Mothersill, Carmel

doi:10.1002/cbf.942

Cited by 3 publications

(1 citation statement)

References 64 publications

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“…The bcl-2 and cmyc primary antibodies used were mouse monoclonals obtained from DAKO Cytomation and Novocastra laboratories, respectively. All were recommended for immunohistochemistry with mouse tissues but have been previously shown to work for rainbow trout ( , ). Immunochemistry was performed using an appropriate Vectastain ABC kit (Burlingame, CA).…”

Section: Methodsmentioning

confidence: 99%

Communication of Radiation-Induced Stress or Bystander Signals between Fish in Vivo

Mothersill

Bucking

et al. 2006

Environ. Sci. Technol.

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We report data in this paper suggesting that fish irradiated to 0.5 Gy total body dose can release factors into the water that signal other unexposed fish and cause induction of bystander effects expressed as increased cell death in a reporter system. Radiation-induced bystander effects, resulting in the appearance of radiation damage or induction of typical radiation responses in unirradiated cells and tissues are now an established consequence of exposure to low doses of ionizing radiation, however little work has been done in vivo or in species other than humans or mice. In these experiments rainbow trout were irradiated and then paired with unirradiated fish for two hours. Additionally, unirradiated fish were placed in water which had previously been used to hold irradiated fish for 2 h. Sham-irradiated fish and absolute control fish were also examined all using blind protocols. Following a two h incubation period, at these various exposure regimes, the fish were killed by a blow to the head and dissected. Five organs were removed from each fish and tissue explants were cultured using an established technique. After 2 days, the culture medium was harvested and used in a reporter assay to determine whether a bystander effect had been induced. The explants were cultured on in Clonetics growth medium for a further 14 days then fixed for assay of radiation response proteins. The responses varied according to the cell type in the original explants, with the gill and fin showing the most pronounced response. The results suggest that communication signals leading to a typical radiation response can be passed between fish and seem to involve secretion of a chemical messenger into the water.

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Section: Methodsmentioning

confidence: 99%

Communication of Radiation-Induced Stress or Bystander Signals between Fish in Vivo

Mothersill

Bucking

et al. 2006

Environ. Sci. Technol.

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Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques

Harris¹,

Lambkin²,

O'Byrne-Ring³

2006

Aquaculture

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Identification and localization of a putative ATP-binding cassette transporter in sea lice (Lepeophtheirus salmonis) and host Atlantic salmon (Salmo salar)

et al. 2007

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Some members of the ABC-transporter superfamily, such as P-glycoprotein and the multidrug resistance associated protein, may confer resistance to the avermectin subclass of macrocyclic lactones. The aim of this study was to examine the presence of ABC transporters in both sea lice (Lepeophtheirus salmonis) and its Atlantic salmon host (Salmo salar) using monoclonal antibodies (C219 and JSB-1, with high selectivity for P-gp) and a new polyclonal antibody (SL0525) generated against a putative sea louse ABC transporter. The antibody raised to SL0525 did not react with rat P-gp, suggesting that an ABC transporter, not necessarily P-gp, was isolated. C219 was the only antibody to localize P-gp in all 3 salmon tissues (intestine, kidney and liver). American lobster (Homarus americanus) was used as a reference crustacean for L. salmonis immunostaining reactions and showed positive staining in the hepatopancreatic and intestinal tissues with all 3 antibodies. The L. salmonis showed positive staining in the intestinal epithelial lining with all antibodies. This report represents the first documented evidence for the expression of ABC transporters in L. salmonis, its Atlantic salmon host, and the American lobster.

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Cross‐reactivity of some antibodies to human epitopes with shrimp Pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro

Cited by 3 publications

References 64 publications

Communication of Radiation-Induced Stress or Bystander Signals between Fish in Vivo

Communication of Radiation-Induced Stress or Bystander Signals between Fish in Vivo

Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques

Identification and localization of a putative ATP-binding cassette transporter in sea lice (Lepeophtheirus salmonis) and host Atlantic salmon (Salmo salar)

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