Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques

Harris, Leanne R.; Lambkin, Helen; O'Byrne-Ring, N.

doi:10.1016/j.aquaculture.2006.09.021

Cited by 7 publications

(6 citation statements)

References 29 publications

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“…Cyclin D1 is widely used as a histopathological marker in many tissues as it an important regulator of the cell cycle progression from G1 to S phase. In normal tissues, its expression is well regulated, while the highly expressed cyclin D1 might be a good surrogate to genotoxicity as it refers to the deregulation of the cell cycle [ 49 , 50 ]. In this research, adult snails that were exposed to sublethal concentrations of C. procera or A. halimus had anomalies in their digestive and hermaphrodite glands.…”

Section: Discussionmentioning

confidence: 99%

“…The molluscicidal activity of C. procera and A. halimus may be related to the presence of high levels of fatty acids, particularly palmitic acid, which has been reported to be responsible for the killing of Pomacea canaliculata snails [ 51 ]. The immunohistochemical analysis relies on specific interactions between antibodies and their target antigens [ 49 , 50 ]. Cyclin D1 controls how far along the cell cycle it is allowed to go.…”

Section: Discussionmentioning

confidence: 99%

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Metabolomic Profiling, Antibacterial, and Molluscicidal Properties of the Medicinal Plants Calotropis procera and Atriplex halimus: In Silico Molecular Docking Study

Morad

El‐Sayed

El‐Khadragy

et al. 2023

Plants

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The potential of plant-based natural compounds in the creation of new molluscicidal and antimicrobial medications has gained attention in recent years. The current study compared the metabolic profiles, antibacterial, and molluscicidal properties of the medicinal plants Calotropis procera (C. procera) and Atriplex halimus (A. halimus). In both plants, 118 metabolites were identified using gas chromatography-mass spectrometry. Palmitic acid, stigmasterol, and campesterol were the most prevalent constituents. C. procera extract showed stronger antibacterial activity than A. halimus against Escherichia coli and Proteus mirabilis. Both extracts exhibited molluscicidal activity against Biomphalaria alexandrina, with LC50 values of C. procera (135 mg/L) and A. halimus (223.8 mg/L). Survival rates of snails exposed to sub-lethal concentrations (LC25) of C. procera and A. halimus extracts were 5% and 20%, respectively. The hatchability of snail eggs exposed to both extracts has been dramatically reduced. Both extracts significantly decreased the levels of alkaline phosphatase, acid phosphatase, total protein, and albumin in snails, as well as causing DNA damage and resulting in numerous hermaphrodite and digestive gland damages and distortions. Molecular docking showed palmitic acid binding with acid, alkaline, and alanine aminotransferases in treated digestive gland snails. In conclusion, C. procera and A. halimus have antibacterial and molluscicidal properties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metabolomic Profiling, Antibacterial, and Molluscicidal Properties of the Medicinal Plants Calotropis procera and Atriplex halimus: In Silico Molecular Docking Study

Morad

El‐Sayed

El‐Khadragy

et al. 2023

Plants

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“…vesicular round to oval large nuclei with evident nucleoli), but showed nuclear PCNA immunostaining in early phases of the disease, while in advanced stages they showed predominantly cytoplasmic labelling. It is known that PCNA is also ex pressed during the cell cycle in proliferating cells of molluscs (Marigómez et al 1999, Hanselmann et al 2000, Harris et al 2006, Franco et al 2010: PCNA levels increase during the G1-S phase of the cell cycle, and usually localize in the nucleus, being involved in cell proliferation control and DNA repair (Paunesku et al 2001). However, recent studies in human granulocytes suggest that PCNA could be also involved in suppressing apoptosis when localized in the cytoplasm, where it binds to and inhibits the procaspases, which are key molecules in apoptosis control (Witko-Sarsat et al 2010); interestingly, this pattern has also been ob - Lanza (1980) and Bartl et al (1984) served in human Hodgkin's lymphoma (Benjamin & Gown 1991).…”

Section: Discussionmentioning

confidence: 99%

“…PCNA immunohistochemistry and quantification were made according to previous reports (Maiolino et al 1995, De Vico et al 1996 with slight modifica- tion, using a mouse monoclonal antibody against PCNA, clone PC10 (Sigma), which was already successfully tested to localize proliferating cells in several mollusc species, including Mytilus galloprovincialis (Mari gómez et al 1999, Hanselmann et al 2000, Harris et al 2006, Franco et al 2010. Briefly, after deparaffinization of the tissue sections, endogenous peroxidase was blocked by incubation in H 2 O 2methanol (4:1) for 20 min.…”

Section: Methodsmentioning

confidence: 99%

Cytomorphology and PCNA expression pattern in bivalves Mytilus galloprovincialis and Cerastoderma edule with haemic neoplasia

Carella

Figueras²,

Novoa³

et al. 2013

Dis. Aquat. Org.

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Haemic neoplasia (HN) is a pathologic condition reported in several bivalve species in different geographic areas. In this study we describe the cytomorphological features and the proliferative behaviour, assessed by the proliferating cell nuclear antigen (PCNA), of HN in common cockle Cerastoderma edule and Mediterranean mussel Mytilus galloprovicialis. In mussels the presence of at least 5 types of atypical haemocytes was detected, including A- and B-type cells, previously described in M. edulis and Mytilus sp., with predominance of A-type cells in early phases of the disease and B-type cells in more advanced stages. PCNA immunostaining was positive for 97 to 100% of the neoplastic cells, with both cytoplasmic (A cells) and nuclear patterns (B cells). Conversely, in C. edule there was no distinctive morphological cell sub-population, and staining atypical haemocytes with PCNA (range 93 to 100%) showed nuclear expression in early phases of disease and cytoplasmic expression in more advanced stages. The above findings suggest distinct histo-pathogenetic pathways for HN in mussels and common cockles.

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“…Interestingly, intersex also shows a positive correlation with PCNA and Avon sampling site (Figure 3). Increased PCNA protein expression has previously been detected in histologically normal (Marigomez et al, 1999;Hanselmann et al, 2000;Harris et al, 2006;Franco et al, 2010) and neoplastic (Carella et al, 2013) mollusc tissues. In contrast, PCNA down regulation has been reported in gill tissue of mussels experimentally exposed to metals (Varotto et al, 2013).…”

Section: Discussionmentioning

confidence: 85%

Intersex related gene expression profiles in clams Scrobicularia plana: Molecular markers and environmental application

Ciocan

Cubero-Leon

Langston

et al. 2015

Marine Pollution Bulletin

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Intersex, the appearance of female characteristics in male gonads, has been identified in several aquatic species. It is a widespread phenomenon in populations of the bivalve, Scrobicularia plana, from the southwest coast of the U.K. Genes previously identified as differentially expressed (ferritin, testicular haploid expressed gene, THEG, proliferating cell nuclear antigen, PCNA; receptor activated protein kinase C, RACK; cytochrome B, CYB; and cytochrome c oxidase 1, COX1) in intersex clams relative to normal male clams, were selected for characterisation and an environmental survey of the Channel region. Transcripts were significantly differentially expressed at sites with varying intersex incidence and contaminant burdens. Significant correlations between specific gene expressions, key contaminants and sampling locations have been identified, though no single gene was associated with intersex incidence. The results highlight the difficulty in understanding the intersex phenomenon in molluscs where there is still a lack of knowledge on the control of normal reproduction.

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Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques

Cited by 7 publications

References 29 publications

Metabolomic Profiling, Antibacterial, and Molluscicidal Properties of the Medicinal Plants Calotropis procera and Atriplex halimus: In Silico Molecular Docking Study

Metabolomic Profiling, Antibacterial, and Molluscicidal Properties of the Medicinal Plants Calotropis procera and Atriplex halimus: In Silico Molecular Docking Study

Cytomorphology and PCNA expression pattern in bivalves Mytilus galloprovincialis and Cerastoderma edule with haemic neoplasia

Intersex related gene expression profiles in clams Scrobicularia plana: Molecular markers and environmental application

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