Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge

Zachariášová, Milena; Cuhra, Petr; Hajšlová, Jana

doi:10.3920/wmj2014.1701

Cited by 23 publications

(23 citation statements)

References 62 publications

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“…Immunochemical methods, based on antigen–antibody reactions, provide rapid and economical alternatives to chromatographic methods and represent one of the most practical options for farmers and industries for a fast and easy control of DON in raw materials and processed food and feed (De Saeger and Van Egmond, ; Zachariasova et al., ). Different formats of immunochemical methods for DON detection have been described in literature and/or are commercially available, including ELISA (Ji et al., ; Hiraoka et al., ), lateral flow test strips (Kolosova et al., ; Huang et al., ; Albert et al., ; Lattanzio et al., ), flow‐through tests (Ediage et al., ), fluorescence polarisation immunoassay (Maragos and Plattner, ; Lippolis et al., ), immunosensors (Romanazzo et al., ; Dorokhin et al., ; Maragos, ; Zhilei et al., ) and IAC (the latter for clean‐up) (Brenn‐Struckhofova et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Different formats of immunochemical methods for DON detection have been described in literature and/or are commercially available, including ELISA (Ji et al., ; Hiraoka et al., ), lateral flow test strips (Kolosova et al., ; Huang et al., ; Albert et al., ; Lattanzio et al., ), flow‐through tests (Ediage et al., ), fluorescence polarisation immunoassay (Maragos and Plattner, ; Lippolis et al., ), immunosensors (Romanazzo et al., ; Dorokhin et al., ; Maragos, ; Zhilei et al., ) and IAC (the latter for clean‐up) (Brenn‐Struckhofova et al., ). However, the ELISA tests still dominate the routine screening practice (Zachariasova et al., ). Recently, a trend towards multi‐mycotoxin detection has been observed for immunochemical methods (Peters et al., ; Ediage et al., ; He et al., ; Lattanzio et al., ; Song et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Antibodies against DON can cross‐react with acetylated and/or modified forms of DON. Cross‐reactivity of DON antibodies with 3‐Ac‐DON and/or 15‐Ac‐DON has been frequently reported with levels ranging between 51–770% and 0–65%, respectively (Tangni et al., ; Sanders et al., ; Zachariasova et al., ). However, very specific 3‐Ac‐DON antibodies have also been described (Sanders et al., ).…”

Section: Discussionmentioning

confidence: 99%

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Risks to human and animal health related to the presence of deoxynivalenol and its acetylated and modified forms in food and feed

Knutsen¹,

Alexander²,

Barregård³

et al. 2017

EFS2

213

187

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Deoxynivalenol (DON) is a mycotoxin primarily produced by Fusarium fungi, occurring predominantly in cereal grains. Following the request of the European Commission, the CONTAM Panel assessed the risk to animal and human health related to DON, 3-acetyl-DON (3-Ac-DON), 15-acetyl-DON (15-Ac-DON) and DON-3-glucoside in food and feed. A total of 27,537, 13,892, 7,270 and 2,266 analytical data for DON, 3-Ac-DON, 15-Ac-DON and DON-3-glucoside, respectively, in food, feed and unprocessed grains collected from 2007 to 2014 were used. For human exposure, grains and grain-based products were main sources, whereas in farm and companion animals, cereal grains, cereal by-products and forage maize contributed most. DON is rapidly absorbed, distributed, and excreted. Since 3-Ac-DON and 15-Ac-DON are largely deacetylated and DON-3-glucoside cleaved in the intestines the same toxic effects as DON can be expected. The TDI of 1 lg/kg bw per day, that was established for DON based on reduced body weight gain in mice, was therefore used as a group-TDI for the sum of DON, 3-Ac-DON, 15-Ac-DON and DON-3-glucoside. In order to assess acute human health risk, epidemiological data from mycotoxicoses were assessed and a group-ARfD of 8 lg/kg bw per eating occasion was calculated. Estimates of acute dietary exposures were below this dose and did not raise a health concern in humans. The estimated mean chronic dietary exposure was above the group-TDI in infants, toddlers and other children, and at high exposure also in adolescents and adults, indicating a potential health concern. Based on estimated mean dietary concentrations in ruminants, poultry, rabbits, dogs and cats, most farmed fish species and horses, adverse effects are not expected. At the high dietary concentrations, there is a potential risk for chronic adverse effects in pigs and fish and for acute adverse effects in cats and farmed mink.This is an open access article under the terms of the Creative Commons Attribution-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited and no modifications or adaptations are made.The EFSA Journal is a publication of the European Food Safety Authority, an agency of the European Union. Deoxynivalenol and its acetylated and modified forms in food and feed www.efsa.europa.eu/efsajournal 2 EFSA Journal 2017;15(9):4718 Deoxynivalenol and its acetylated and modified forms in food and feed www.efsa.europa.eu/efsajournal 3 EFSA Journal 2017;15(9):4718 Deoxynivalenol and its acetylated and modified forms in food and feed www.efsa.europa.eu/efsajournal 4 EFSA Journal 2017;15(9):471870% of ingested DON is excreted via urine, of which about 80% was in conjugated forms, mainly as DON-15-glucuronide that was about threefold more efficiently formed than DON-3-glucuronide. After a single oral exposure to DON, feed refusal appeared very quickly in mice. Previous risk assessments of DON conducted by the Scientific Committee on Food (SCF) in 1999 and by the Joint FAO/WHO Expert Committee on Food Additives...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Risks to human and animal health related to the presence of deoxynivalenol and its acetylated and modified forms in food and feed

Knutsen¹,

Alexander²,

Barregård³

et al. 2017

EFS2

213

187

View full text Add to dashboard Cite

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“…These methods are generally rapid, inexpensive, and relatively easy to use compared to reference chromatography methods, with acceptably low limits of detection [19]. Antibodies used for ELISA have cross reactivity to multiple mycotoxins, such as for AFB1, AFB2, AFG1, and AFG2, therefore making them more appropriate for total AF or FM quantification than specific toxins, e.g., AFB1, which would have affected detection thresholds and overestimation of results [39]. Yet, some immunochemical methods can have a binding specificity specifically to AFB1 [40].…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

A Review of the Methodology of Analyzing Aflatoxin and Fumonisin in Single Corn Kernels and the Potential Impacts of These Methods on Food Security

Chavez

Cheng

Stasiewicz

2020

Foods

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Current detection methods for contamination of aflatoxin and fumonisin used in the corn industry are based on bulk level. However, literature demonstrates that contamination of these mycotoxins is highly skewed and bulk samples do not always represent accurately the overall contamination in a batch of corn. Single kernel analysis can provide an insightful level of analysis of the contamination of aflatoxin and fumonisin, as well as suggest a possible remediation to the skewness present in bulk detection. Current literature describes analytical methods capable of detecting aflatoxin and fumonisin at a single kernel level, such as liquid chromatography, fluorescence imaging, and reflectance imaging. These methods could provide tools to classify mycotoxin contaminated kernels and study potential co-occurrence of aflatoxin and fumonisin. Analysis at a single kernel level could provide a solution to the skewness present in mycotoxin contamination detection and offer improved remediation methods through sorting that could impact food security and management of food waste.

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“…As a complementation, rapid diagnostic methods are commonly based on immunochemical assays (i.e., lateral flow devices, dipsticks, etc.) for early detection at pre-and post-harvest [13]. More recently, nondestructive imaging methods have been proposed as well as rapid diagnostic tool [14].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Approaches and their Hidden Potential for Explaining the Mycotoxin Contamination Problem

Righetti¹,

Dall’Asta²,

Hajšlová³

et al. 2016

Metabolomics - Fundamentals and Applications

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Food is essential for life. On the basis of the previous sentence, consumers have a right to expect that the foods they purchase and consume will be safe, authentic and of high quality. On these premises, target compounds, such as mycotoxins, pesticides or antibiotics, have been commonly investigated on the food chain, and subsequently, were regulated by authorities. This raises the following question: may consumer be prevented to these risk exposures? Probably not, food chain is step-by-step longer and more complex than ever before. Note that food chain is affected by globalized trade, culture, travel and migration, an ageing population, changing consumer trends and habits, new technologies, emergencies, climate change and extreme weather events which are increasing foodborne health risks, especially for mycotoxins. Because of the fact that mycotoxins are natural toxic compounds produced by certain filamentous fungi on many agricultural communities. In fact, these toxins have adverse effects on humans, animals and crops that result in illnesses and economic losses. Nevertheless, so far mycotoxins and their modified forms have been mainly monitored in cereal and cerealbased products, however, may an early detection of mycotoxins be considered a reliable strategy? In this chapter, recent metabolomics approaches have been reviewed in order to answer this question and to understand future strategies in the field of mycotoxin contamination.

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Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge

Cited by 23 publications

References 62 publications

Risks to human and animal health related to the presence of deoxynivalenol and its acetylated and modified forms in food and feed

Risks to human and animal health related to the presence of deoxynivalenol and its acetylated and modified forms in food and feed

A Review of the Methodology of Analyzing Aflatoxin and Fumonisin in Single Corn Kernels and the Potential Impacts of These Methods on Food Security

Metabolomics Approaches and their Hidden Potential for Explaining the Mycotoxin Contamination Problem

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