Cross-reactivity between cowpox and vaccinia viruses with monoclonal antibodies

Abstract

Hybridoma cell lines producing monoclonal antibodies against cowpox virus (CPV) and vaccinia virus (VV) were established to examine the specific and cross-reactive antigenic determinants of these viruses. Monoclonal antibodies against CPV (LB red strain) and VV (Lister strain and Ikeda strain) were classified into several groups on the basis of the results of immunofluorescence and haemagglutination inhibition tests. It was suggested that the groups defined above include the group of antibodies reacting with each of known major antigens of poxvirus, i.e. nucleoprotein (NP) antigen, heat labile and stable complex (LS) antigen, haemagglutinin (HA), cell surface antigen (CSA) and type A inclusion body (A).

Characterization of the gene encoding the A-type inclusion protein of camelpox virus and sequence comparison with other orthopoxviruses

“…Despite the absence of morphologically defined ATIBs, ATI-related proteins with apparent relative molecular masses ranging from 92K to 96K have been described for MP, vaccinia and variola viruses (Patel et al, 1986;Fenner et al, 1989;DeCarlos & Paez, 1991). Monoclonal antibodies cross-react with ATIPs of either CoP, ectromelia and vaccinia viruses or CoP and ectromelia viruses (Kitamoto et al, 1984(Kitamoto et al, , 1986a, indicating a close relationship. Sequence comparison of the genes encoding the 160K ATIP of CoP virus strain Brighton (BR) (Funahashi et al, 1988) and the 94K ATIP of vaccinia virus strain Western Reserve (WR) revealed extensive similarity.…”

Monoclonal Antibodies to Cowpox Virus: Polypeptide Analysis of Several Major Antigens

“…Since the CS1 antibody obtained in this study reacted with cells infected with the VV Lister strain, it may recognize the minor early CS antigen, or one of the several CS antigens. We have also reported that hybridomas obtained from mice immunized with the VV Ikeda strain may recognize the minor CS antigen (Kitamoto et al, 1984). The A212 monoclonal antibody reacted with cytoplasmic ATI in CPV-or EV-infected cells, but not in VV-or SFV-infected cells in the IF test (Table 1).…”

“…Other clones could not be identified, because their reactivities varied under the test conditions used. Most antibodies were characterized according to their IF staining patterns and biological tests as described previously (Kitamoto et al, 1984). However, some antibodies did not react with any polypeptide by either immunoprecipitation or immunoblotting and others reacted with not only major polypeptides but also several unidentified minor polypeptides on immunoprecipitation and/or immunoblotting.…”

“…Our monoclonal antibody reacted with the 100K polypeptide in both CPV-infected cells and VVinfected cells. Moreover, we obtained hybridomas from mice immunized with the VV Lister strain that showed surface-diffuse staining for IF (Kitamoto et al, 1984) and reacted with the 100K polypeptide in both ! CPV-and VV-infected cells (unpublished data).…”

“…RK13 or HeLa cells were infected with CPV, VV or SFV, and HeLa or L cells were infected with EV. The methods for production and culture of hybridomas and the screening procedures for antibodies have been described previously (Kitamoto et al, 1984). Hybridomas were obtained from cultures of myeloma cells fused with spleen cells from mice immunized with cells infected with the LB red strain of CPV.…”