Cross-neutralizing Antibodies Induced by Single Serotype Vaccination of Cows with Rotavirus

Brüssow, Harald; Walther, Isabelle; Fryder, Vincent; Sidoti, Josette; Bruttin, Anne

doi:10.1099/0022-1317-69-7-1647

Cited by 36 publications

(17 citation statements)

References 26 publications

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“…Browning et al have reported that mares immunized with a single-type (G6) inactivated bovine rotavirus vaccine develop antibodies in the serum and milk not only to the G6 type but also to the G2 and the G3 type [26]. Such a heterotypic response has been also reported in adult cows [27,28]. Our present result indicates that the G3BP[12] vaccine would also induce virus-neutralizing antibody against heterologous G14P[12] viruses.…”

Section: Resultssupporting

confidence: 79%

Antibody response in vaccinated pregnant mares to recent G3BP[12] and G14P[12] equine rotaviruses

et al. 2012

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BackgroundBoth the G3P[12] and the G14P[12] type of equine group A rotavirus (RVA) have recently become predominant in many countries, including Japan. G3 types are classified further into G3A and G3B. The G3A viruses have been circulating in Europe, Australia, and Argentina, and the G3B viruses have been circulating in Japan. However, only an inactivated vaccine containing a single G3BP[12] strain is commercially available in Japan. To assess the efficacy of the current vaccine against recently circulating equine RVA strains, we examined antibody responses in pregnant mares to recent G3BP[12] and G14P[12] strains by virus neutralization test.FindingsAfter vaccination in five pregnant mares, the geometric mean serum titers of virus-neutralizing antibody to recent G3BP[12] strains increased 5.3- to 7.0-fold and were similar to that against homologous vaccine strain. Moreover, antibody titers to recent G14P[12] strains were also increased 3.0- to 3.5-fold.ConclusionsThese results suggest that inoculation of mares with the current vaccine should provide foals with virus-neutralizing antibodies against not only the G3BP[12] but also the G14P[12] RVA strain via the colostrum.

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Section: Resultssupporting

confidence: 79%

Antibody response in vaccinated pregnant mares to recent G3BP[12] and G14P[12] equine rotaviruses

et al. 2012

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“…Symptoms are generally severe only when infants aged between 6 and 24 months acquire a primary infection [Soenarto et al, 1981]. Cross-protection has been demonstrated with heterotypic infection of group A rotavirus [Bridger et al, 1987;Brussow et al, 1988;Feng et al, 1994]. Studies with recombinant outer capsid proteins VP4 and VP7 will help determine whether cross protection between rotavirus groups occurs and whether a prior exposure to group A rotavirus attenuates symptoms of subsequent group C rotavirus infection.…”

Section: Discussionmentioning

confidence: 97%

Seroepidemiology of human group C rotavirus in the UK

James

Lambden

Caul³

et al. 1997

J. Med. Virol.

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The gene coding for the major inner capsid protein VP6 of human group C rotavirus was cloned into baculovirus using the pBlueBac2 vector and expressed in insect cells. When cultured in High Five cells, VP6 was expressed at a high level and exported to the cell culture medium. Purified VP6 was used to immunise rabbits. Hyperimmune rabbit serum, which reacted with native human group C rotavirus in infected cells, was used to develop and optimise an EIA for the detection of antibodies to group C rotavirus using the recombinant VP6 as a source of antigen. In a local epidemiological survey of 1000 sera grouped by age, an average of 43% of samples were found to have antibodies to human group C rotavirus with the highest proportion (66%) in the 71-75 year age group. In comparison, 97% of adults and 85% of children had antibodies to recombinant VP6 from the bovine RF strain of group A rotavirus. These results suggest that infection with human group C rotavirus is a common occurrence despite the apparent rarity of reports of human group C rotavirus in clinical samples from patients with gastroenteritis.

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“…TLP ( 125 I labeling, in the presence (ϩ) or absence (Ϫ) of BSA. Electron microscopy was performed as described previously, using ammonium molybdate/ bacitracin negative staining (6). amide (Fig.…”

Section: Resultsmentioning

confidence: 99%

Glycosphingolipid Binding Specificities of Rotavirus: Identification of a Sialic Acid-Binding Epitope

Delorme

Brüssow

Sidoti

et al. 2001

J Virol

127

143

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The glycosphingolipid binding specificities of neuraminidase-sensitive (simian SA11 and bovine NCDV) and neuraminidase-insensitive (bovine UK) rotavirus strains were investigated using the thin-layer chromatogram binding assay. Both triple-layered and double-layered viral particles of SA11, NCDV, and UK bound to nonacid glycosphingolipids, including gangliotetraosylceramide (GA1; also called asialo-GM1) and gangliotriaosylceramide (GA2; also called asialo-GM2). Binding to gangliosides was observed with triple-layered particles but not with double-layered particles. The neuraminidase-sensitive and neuraminidase-insensitive rotavirus strains showed distinct ganglioside binding specificities. All three strains bound to sialylneolactotetraosylceramide and GM2 and GD1a gangliosides. However, NeuAc-GM3 and the GM1 ganglioside were recognized by rotavirus strain UK but not by strains SA11 and NCDV. Conversely, NeuGc-GM3 was bound by rotaviruses SA11 and NCDV but not by rotavirus UK. Thus, neuraminidase-sensitive strains bind to external sialic acid residues in gangliosides, while neuraminidase-insensitive strains recognize gangliosides with internal sialic acids, which are resistant to neuraminidase treatment. By testing a panel of gangliosides with triple-layered particles of SA11 and NCDV, the terminal sequence sialyl-galactose (NeuGc/NeuAc␣3-Gal␤) was identified as the minimal structural element required for the binding of these strains. The binding of triple-layered particles of SA11 and NCDV to NeuGc-GM3, but not to NeuAc-GM3, suggested that the sequence NeuGc␣3Gal␤ is preferred to NeuAc␣3Gal␤. Further dissection of this binding epitope showed that the carboxyl group and glycerol side chain of sialic acid played an important role in the binding of such triple-layered particles.

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Cross-neutralizing Antibodies Induced by Single Serotype Vaccination of Cows with Rotavirus

Cited by 36 publications

References 26 publications

Antibody response in vaccinated pregnant mares to recent G3BP[12] and G14P[12] equine rotaviruses

Antibody response in vaccinated pregnant mares to recent G3BP[12] and G14P[12] equine rotaviruses

Seroepidemiology of human group C rotavirus in the UK

Glycosphingolipid Binding Specificities of Rotavirus: Identification of a Sialic Acid-Binding Epitope

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