Cross-Linking of Gelatin and Chitosan Complex Nanofibers for Tissue-Engineering Scaffolds

Abstract

The aim of this study is to investigate cross-linked gelatin-chitosan nanofibers produced by means of electrospinning. Gelatin and chitosan nanofibers were electrospun and then cross-linked by glutaraldehyde (GTA) vapor at room temperature. Scanning electron microscopy (SEM) images showed that the cross-linked mats could keep their nanofibrous structure after being soaked in deionized water at 37° C. The cross-linking mechanism was discussed based on FT-IR results. The two main mechanisms of cross-linking for chitosan and gelatin-chitosan complex are Schiff base reaction and acetalization reaction. For gelatin, the mechanism of cross-linking was Schiff base reaction. The mechanical properties of nanofibrous mats were improved after cross-linking. The biocompatibility of electrospun nanofibrous mats after cross-linking was investigated by the viability of porcine iliac endothelial cells (PIECs). The morphologies of PIECs on the cross-linked nanofibrous mats were observed by SEM. In addition, proliferation of PIECs was tested with the method of methylthiazol tetrazolium (MTT) assay. The results indicate that gelatin-chitosan nanofibrous mats could be a promising candidate for tissue-engineering scaffolds.

Emulsion cross-linked chitosan/nanohydroxyapatite microspheres for controlled release of alendronate

“…The peaks at 2,924 and 2,846 cm -1 were typical of C-H stretch vibration. The sharp peaks at 1,383 cm -1 were assigned to characteristic stretching vibration of C=N, which can be attributed to the Schiff base reaction between the aldehyde group of glutaraldehyde and amino group of CH, consistent with the observation in the previous study [41]. The PO 4 3-bands belonging to nHA were located at 604, 961, 1,040 and 1,097 cm -1 in CH/nHA-AL (Fig.…”

Eco-friendly Electrospun Polymeric Nanofibers-Based Nanocomposites for Wound Healing and Tissue Engineering

“…Upon connecting the high applied voltage (low electric current) between the tip of the spinneret and the collector situated precisely in front of the tip of the spinneret, an intense electrification of the first polymer droplet forced out of the syringe under the influence of the adjusted controlled flow rate occurs (Qian et al 2011).…”

“…Chitosan has proven to be efficient in stimulating the growth of diverse types of cells such as endothelial cell, fibroblasts, hepatocytes, neural cells and others (Jafari et al 2011;Jiankang et al 2009;Nisbet et al 2008;Qian et al 2011).…”

“…Gelatin has been also blended successfully with several polymers to fabricate tissue engineered nanofibrous scaffolds. An example, is its incorporation with chitosan to obtain chitosan/gelatin hybrid nanofibers which were tested successfully with variety of different cells like fibroblasts, dermal cells, hepatocytes, and porcine endothelial cells (Dhandayuthapani et al 2010;Jafari et al 2011;Jiankang et al 2009;Qian et al 2011).…”

In vitro models for the evaluation of angiogenic potential in bone engineering

“…Calcein-acetoxymethylester becomes fluorescent when taken up by viable cells and the fluorescence is spread throughout the cell. Alternatively the cell number can be evaluated with tritiated thymidine [94] , MTT [95] , or crystal violet [96] assays.…”