Cross-linking method using pentaepoxide for improving bovine and porcine bioprosthetic pericardia: A multiparametric assessment study

Zhuravleva, I. Yu.; Карпова, Е. В.; Опарина, Л. А.; Poveschenko, Olga V.; Суровцева, М. А.; Титов, А. Т.; Ksenofontov, Alexander L.; Vasilieva, Maria B.; Кузнецова, Е. В.; Bogachev-Prokophiev, Alexander V; Трофимов, Б. А.

doi:10.1016/j.msec.2020.111473

Cited by 18 publications

(18 citation statements)

References 52 publications

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“…The changes in thermal stability that we have obtained fit into the regularity that is well‐known for biological materials: T d (GA) > T d (epoxides) > T d (fresh). This pattern has been confirmed in several previous studies, 5,26,33,42 but without any explanation.The revealed effects cannot be explained by differences in the cross‐link density, contrary to the well‐known postulate that the higher the cross‐link density, the higher is the strength and thermal stability 31,43 . The highest cross‐linking density was provided by DE (Figure 4).…”

Section: Discussionsupporting

confidence: 86%

“…For hydrated collagen type I, as well as for fresh xenogeneic pericardium, mainly consisting of this collagen, the denaturation temperature is in the range of 70 C-79 C, and the denaturation enthalpy is 50-70 J/g. 26,44,49 The denaturation temperature of fresh BJVW is several degrees lower ( 42 and our results), probably due to the highly hydrated elastin. 48 It is believed that the denaturation of noncrosslinked hydrated collagen is mainly due to the breaking of hydrogen bonds that form a hydration network around collagen molecules.…”

Section: Discussionmentioning

confidence: 53%

“…However, it was demonstrated that epoxy-treated bovine and porcine pericardia have inferior mechanical characteristics with their GA-counterparts: they are stiffer, but less durable. 25,26 It is not known whether this is true for a BJVW, because the BJV conduit is an "orphan" bioprosthetic material and its biomechanics is very poorly understood. At least three studies [27][28][29] have been devoted to BJV valves, and there is only one piece of information on fresh BJVW behavior in the available literature, 30 whereas the pericardium biomechanics, for example, has been covered in numerous studies and reviews.…”

Section: Introductionmentioning

confidence: 99%

“…However, it was demonstrated that epoxy‐treated bovine and porcine pericardia have inferior mechanical characteristics with their GA‐counterparts: they are stiffer, but less durable 25,26 . It is not known whether this is true for a BJVW, because the BJV conduit is an “orphan” bioprosthetic material and its biomechanics is very poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Bovine jugular vein conduit: What affects its elastomechanical properties and thermostability?

Zhuravleva

Карпова

Dokuchaeva

et al. 2021

J Biomedical Materials Res

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The aim of this study was to compare the mechanical properties and thermal stability of the venous wall depending on the treatment method used, and, accordingly, on those structural changes in the tissue that this treatment causes. Bovine jugular vein walls (BJVWs) cross-linked with glutaraldehyde (GA), ethylene glycol diglycidyl ether (DE), and Contegra commercial conduit were evaluated using uniaxial stretching [with and without pre-conditioning (PreC)], differential scanning calorimetry, amino acid analysis, and attenuated total reflection infrared spectroscopy. Fresh BJVW was used as a control. It was shown that failure stress in non-PreC GA-treated and DE-treated materials was lower than that in fresh and Contegra counterparts. Contegra samples were

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bovine jugular vein conduit: What affects its elastomechanical properties and thermostability?

Zhuravleva

Карпова

Dokuchaeva

et al. 2021

J Biomedical Materials Res

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“…Favorsky Irkutsk Institute of Chemistry, Siberian Branch of the Russian Academy of Sciences (Russia) in 1985. The assessment of cytotoxicity of PEpreserved bioprosthetic materials was given in one work only and in regard to only one kind of cells [8].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Xenogeneic Pericardium Preserved by Epoxy Cross-Linking Agents

Bondarenko¹,

Суровцева²,

Лыков³

et al. 2021

Sovrem Tehnol Med

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The aim of the study was to assess the cytotoxic effect of xenopericardial biomaterial treated with di-and pentaepoxides on the cell cultures in vitro.Materials and Methods. Samples of bovine and porcine pericardium were used in the work. Three different modes were employed for preservation: 1) 0.625% solution of glutaraldehyde (GA) and a two-fold change on days 2 and 7; 2) 5% solution of ethylene glycol diglycidyl ether (EGDE) changed on day 2; 3) 5% EGDE solution for 10 days, then 2% pentaepoxide solution also for 10 days. The cytotoxicity of the biomaterial was assessed by the extraction method. To determine the cytotoxicity of the biomaterial, EA.hy926 cells, multipotent mesenchymal stem cells (MMSCs), and fibroblasts were used. Cell viability was determined by the MTT test. The level of apoptosis and necrosis in the cell cultures was assessed by staining with acridine orange and ethidium bromide after cultivation with xenopericardial extracts employing different modes of preservation.Results. Extracts of bovine and porcine pericardium preserved with GA have been found to have the greatest toxic effect on the cell cultures showing 20-33% reduction of cell viability. Extracts from bovine and porcine pericardium preserved with di-and pentaepoxy compounds do not have a toxic effect on endothelial cells, MMSCs, and fibroblasts since cell viability reduction is by no more than 15%. The lowest level of apoptosis and necrosis is observed in the cell cultures under the influence of extracts from the pericardium, preserved with diepoxide and pentaepoxide compounds. Conclusion.According to the MTT test for cytotoxicity and determination of the level of apoptosis and necrosis in cell cultures, bovine and porcine pericardia treated with di-and pentaepoxides have been established to have no cytotoxic effect on the culture of endothelial EA.hy926 cells, MMSCs, fibroblasts in vitro, whereas GA, in comparison with di-and pentaepoxides, has a toxic impact on the cells.

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Well-designed two-fold crosslinked biological valve leaflets with heparin-loaded hydrogel coating for enhancing anticoagulation, endothelialization, and anticalcification

Yue³

et al. 2022

Biomater. Sci.

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Cross-linking method using pentaepoxide for improving bovine and porcine bioprosthetic pericardia: A multiparametric assessment study

Cited by 18 publications

References 52 publications

Bovine jugular vein conduit: What affects its elastomechanical properties and thermostability?

Bovine jugular vein conduit: What affects its elastomechanical properties and thermostability?

Cytotoxicity of Xenogeneic Pericardium Preserved by Epoxy Cross-Linking Agents

Well-designed two-fold crosslinked biological valve leaflets with heparin-loaded hydrogel coating for enhancing anticoagulation, endothelialization, and anticalcification

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