Cross-linked enzyme aggregates of recombinant Pseudomonas putida nitrilase for enantioselective nitrile hydrolysis

Characterization, amyloid formation, and immobilization of a novel SGNH hydrolase from Listeria innocua 11262

“…Thermal stability of LI22-CLEAs can be important for chemical processes conducted at high temperatures. The CLEAs of other enzymes also showed thermal stability as compared to soluble enzyme [30][31][32]. Fig.…”

Kinetics and mechanism of regioselective monoacetylation of 3-aryloxy-1,2-propandiols using immobilized Candida antarctica lipase

“…The prevalent application of biocatalysis in non-aqueous 10 media has included stereo-selective synthesis of enantiopure 11 drugs over the past two decades [1][2][3][4]. The production of 12 enantiomerically pure compounds has gained significant impor- 13 tance in chemical and pharmaceutical industry.…”

Solid state fermentation with recovery of Amyloglucosidase from extract by direct immobilization in cross linked enzyme aggregate for starch hydrolysis

“…CLEA was formed by precipitation of enzyme and other proteins (impurities) coupled with cross-linking as described in literature (Kumar et al, 2010). 1 ml enzyme extract (2.16 mg protein, 1.3 U AMG activity) was mixed with appropriate amount of cross-linker to give desired final concentration and volume was made to 8 ml by 50 mM phosphate buffer (pH 7.0).…”