Cross-linked acellular lung for application in tissue engineering: Effects on biocompatibility, mechanical properties and immunological responses

Shirani, Ali; Ganji, Fatemeh; Golmohammadi, Mahtab; Hashemi, Seyed Mahmoud; Mozafari, Masoud; Amoabediny, Ghassem; Osguei, Nushin Karkuki; Samadikuchaksaraei, Alí

doi:10.1016/j.msec.2021.111938

Cited by 10 publications

(13 citation statements)

References 32 publications

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“…Meanwhile, with utilization of carboxyl groups from Asp, hEGF were successfully crosslinked to DPP-Asp ( Figure 4(c) ). Prior reports showed that crosslinked tissues had biological properties closer to those of native tissues [ 16 ], and therefore, the cell sheet cultured on these tissues was considered ideal. Meanwhile, chemical crosslinking will significantly inhibit the degradation of biological scaffolds and alter the host tissue response to the biological material [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Prior reports showed that crosslinked tissues had biological properties closer to those of native tissues [ 16 ], and therefore, the cell sheet cultured on these tissues was considered ideal. Meanwhile, chemical crosslinking will significantly inhibit the degradation of biological scaffolds and alter the host tissue response to the biological material [ 16 ]. Asp is a nontoxic, biocompatible, and biodegradable protein widely expressed in the human body and may help to promote damage repair and the production of extracellular matrix [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although the decellularization technique has been studied for a long time, there is no standard protocol. It is suggested to make a different protocol of decellularization for each specific tissue and it is necessary to complement with a postdecellularization process, to reduce the loss and damage of ECM in the process of decellularization [ 16 ]. Previous studies showed that crosslinking can improve mechanical, structural, and biological properties and biodegradation rate [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is suggested to make a different protocol of decellularization for each specific tissue and it is necessary to complement with a postdecellularization process, to reduce the loss and damage of ECM in the process of decellularization [ 16 ]. Previous studies showed that crosslinking can improve mechanical, structural, and biological properties and biodegradation rate [ 16 , 17 ]. 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) is a low-toxicity or not cytotoxic chemical that has been widely applied for crosslinking [ 16 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies showed that crosslinking can improve mechanical, structural, and biological properties and biodegradation rate [ 16 , 17 ]. 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) is a low-toxicity or not cytotoxic chemical that has been widely applied for crosslinking [ 16 , 18 ]. EDC, a carboxylic acid group activation, could form amide bonds by coupling with reactive intermediates and the amino group [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Chen

Deng

Luo

et al. 2022

Stem Cells International

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Seeking for suitable conjunctival reconstruction substitutes to overcome the limitations of current substitutes, such as amniotic membrane, is urgent. Decellularized tissues have become a promising strategy for tissue engineering. In this study, we prepared decellularized porcine pericardium (DPP) scaffolds by the phospholipase A2 method and crosslinked them with aspartic acid (Asp) and human endothelial growth factor (hEGF) to enhance biological performance on the DPP, obtaining DPP-Asp-hEGF scaffolds. In vitro DPP showed lower apoptosis, highly desirable, well preservation of extracellular matrix components, and favorable macro-microstructure, which was confirmed by histology, immunofluorescence, electron microscopy, collagen and DNA quantification, and cytotoxicity assay, compared to the native porcine pericardium (NPP). The crosslinked efficacy of the DPP-Asp-hEGF was furtherer verified by in vitro experiments with Fourier transform infrared (FTIR) and X-ray diffraction (XRD). Through animal models of conjunctiva defect model, the DPP-Asp-hEGF revealed a closed, multilayer epithelium with an equal amount of goblet cells and no indication for conjunctival scarring after 28 days, compared to amniotic membrane (AM) groups and sham groups. These results suggested that DPP-Asp-hEGF can offer a good conjunctival reconstructive substitute both in structure and in function.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Chen

Deng

Luo

et al. 2022

Stem Cells International

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Crosslinking strategies of decellularized extracellular matrix in tissue regeneration

Qiao,

Peijie,

Nan

2023

J Biomedical Materials Res

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By removing the immunogenic cellular components through various decellularization methods, decellularized extracellular matrix (dECM) is considered a promising material in the field of tissue engineering and regenerative medicine with highly preserved physicochemical properties and superior biocompatibility. However, decellularization treatment can lead to some loss of structural integrity, mechanical strength, degradation stability, and biological performance of dECM biomaterials. Therefore, physical and chemical crosslinking methods are preferred to restore or even improve the biomechanical properties, stability, and bioactivity, and to achieve a delicate balance between degradation of the implanted biomaterial and regeneration of the host tissue. This review provides an overview of dECM biomaterials, and describes and compares the mechanisms and characteristics of commonly used crosslinking methods for dECM, with a focus on the potential applications of versatile dECM‐based biomaterials derived from skin, cardiac tissues (pericardium, heart valves, myocardial tissue), blood vessels, liver, and kidney, modified with different chemical crosslinking reagents, in tissue and organ regeneration.

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Effect of Composition of Lung Biomimetic Niche on the Mesenchymal Stem Cell Differentiation toward Alveolar Type II Pneumocytes

Sala

Gennaro

Lista

et al. 2023

Macromolecular Bioscience

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Pulmonary niche dynamically orchestrates the signals, such as proliferation or differentiation of mesenchymal stem cells (MSCs), which allows inducing tissue repair. Lung niche includes extracellular matrix (ECM), comprising hyaluronic acid (HA) and collagen (COLL), and several types of MSCs. Impaired ECM, in lung pathologies, makes the promising therapies based on MSCs ineffective, as it results in a reduced attachment and homing of MSCs, precluding their differentiation and viability. To overcome this problem, in this study a pulmonary biomimetic niche based on HA and COLL hydrogel is developed, with the specific aim to elucidate the role of COLL and HA/COLL semi-interpenetrating polymer networks (SIPNs) in directing the differentiation of MSCs into Alveolar Type II (ATII) cells. The effect of low (L), medium (M), and high (H) molecular weight (MW) HA is investigated, both like structural component of the SIPNs hydrogel and like trophic factor in cell culture media solution. HA in the culture media significantly improves surfactant protein (SP)-C expression (≈2 ng mL −1 ), without showing difference in the MW tested, compared to control only (≈1 ng mL −1 ). Furthermore, LMWHA/COLL hydrogel promotes the SPC expression (approximately two times) compared to COLL, MMWHA/COLL, and HMWHA/COLL hydrogels.

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Cross-linked acellular lung for application in tissue engineering: Effects on biocompatibility, mechanical properties and immunological responses

Cited by 10 publications

References 32 publications

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Crosslinking strategies of decellularized extracellular matrix in tissue regeneration

Effect of Composition of Lung Biomimetic Niche on the Mesenchymal Stem Cell Differentiation toward Alveolar Type II Pneumocytes

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