Cross-axis synchronous flow-through coil planet centrifuge (type XLL)

Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge

“…In the early 1980s a great advance in the CCC technology was made by the discovery of a new hydrodynamic phenomenon in a rotating coiled tube [10], which provided a basis for developing a highly efficient CCC system called high-speed CCC (HSCCC) [11]. The cross-axis coil planet centrifuge (CPC) with the column holders shifted laterally along the holder shaft enables retention of the stationary phase of aqueousaqueous two-phase systems, with the aid of the laterally acting strong centrifugal field [12,13].…”

Aqueous–aqueous two-phase systems composed of low molecular weight of polyethylene glycols and dextrans for counter-current chromatographic purification of proteins

“…Consequently, the system eliminates various complications arising from the use of solid supports. The cross-axis coil planet centrifuge (CPC) allows satisfactory levels of stationary phase retention for the aqueous-aqueous polymer phase systems so that it can be effectively used for the preparative separation of proteins [7,8]. With PEG-potassium phosphate systems, the cross-axis CPC has been successfully used for the separations of a variety of protein samples, including a mixture of cytochrome c, myoglobin, ovalbumin and hemoglobin [9], human plasma lipoproteins (HDL, LDL and VLDL) [10][11][12], cholinesterase from human serum [13], lactic acid dehydrogenase from bovine heart crude extract [14,15], alcohol dehydrogenase from bovine liver crude extract [16], various recombinant enzymes [17,18] from E. coli lysate.…”

Purification of glucosyltransferase from cell-lysate of Streptococcus mutans by counter-current chromatography using aqueous polymer two-phase system

“…Its unique feature, among other chromatographic systems, is derived from the fact that the method uses no solid support, and the liquid stationary phase is retained in a multilayer coiled tube aided by an Archimedean screw effect induced by a rotating centrifugal force field. For protein separations, several types of cross-axis coil planet centrifuges (X-axis CPCs) have been designed for performing CCC using highly viscous polymer two-phase systems such as polyethylene glycol (PEG)-potassium phosphate buffer system and PEG-dextran system [20][21][22]. These X-axis CPCs have been successfully used for the separation and purification of a variety of physiological proteins including histones and serum proteins [23], recombinant uridine phosphorylase [24], human lipoproteins [25], lactic acid dehydrogenase [26], chicken egg white proteins [27], cholinesterase [28] and single-strand DNA binding protein [29].…”