Cross-antigenicity Between Ruminal and Hepatic Paramphistomes and Liver Flukes of Buffalo Origin

Maji, B. P.; Dwivedi, P.; Rao, J. R.; Yadav, Shobha

doi:10.1080/09712119.1999.9706262

Cited by 5 publications

(7 citation statements)

References 3 publications

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“…The probability of diagnosis of animals infected with paramphistomes is more by detecting the circulating antibodies in the sera samples of infected animals as compared to the functional/structural antigen of the parasite by ELISA (Maji et al 1999). However, the success of the diagnostic test depending upon the antibody detection depends heavily on identification of suitable antigen specific for paramphistomosis, because the efficacy of immunodiagnostic test is hampered by the possession of common antigenic epitopes by various helminths, thus leading to lack of specificity.…”

Section: Resultsmentioning

confidence: 99%

Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum

et al. 2010

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Polypeptide profile of somatic antigen of Paramphistomum epiclitum (PSAg) and Gastrothylax crumenifer (GSAg) was studied by SDS-PAGE. PSAg and GSAg showed 14 and 19 polypeptides in the range of 14.9-95.5 and 13.7-129.6 kDa with six common polypeptides of mol wt 16.8, 21.8, 23.7, 35.5, 43.4 and 70.8 kDa. P. epiclitum experimentally infected sheep sera were used for identification of specific immuno-dominant peptide in the range of 37-40 kDa against P. epiclitum by western blotting. Hyperimmune sera (HIS) was raised in rabbit against the identified polypeptide, IgG was separated from HIS and an immunoaffinity column was constructed with a binding percentage of 83.74 of IgG with CNBr activated Sepharose 4B. Purification of somatic antigen (PSAg) was done with immunoaffinity chromatography and 37-40 kDa protein antigen was isolated in pure form with recovery percentage of 2.97%. This purified fraction of somatic antigen can be used as a candidate antigen for development of serological assay for early diagnosis of paramphistomosis among livestock.

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Section: Resultsmentioning

confidence: 99%

Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum

et al. 2010

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“…The probability of diagnosis of animals infected with amphistomes is more by detecting the circulating antibodies in the sera samples of infected animals as compared to the functional/structural antigen of the parasite by ELISA [ 13 ]. It is interesting to note that studies on immunodiagnosis of amphistomosis have been mostly based on either adult worm somatic antigen [ 13 – 17 ], excretory/secretory antigen [ 11 , 18 ], or coproantigen [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding immunodiagnosis of amphistomosis limited work has been done utilizing either adult worm somatic antigen [ 13 – 17 ], excretory/secretory antigen [ 11 , 18 ] or coproantigen [ 12 ] as antigen. It is also well known that helminth parasites during the course of development undergo antigenic polymorphism which induces drastic alterations in immune response, so use of these different developmental stage antigens is very important in immunodiagnosis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antibody Response to Various Developmental Stage Specific Somatic Antigens ofParamphistomum epiclitumin Goats

Kumari

Prasad

Singh

2014

BioMed Research International

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Electrophoretic analysis of various developmental stage specific somatic antigens of Paramphistomum epiclitum (Digenea: Paramphistomidae), namely, metacercariae (McAg), immature intestinal flukes (ImIAg), immature ruminal flukes (ImRAg), and adult flukes (AAg), was done by native polyacrylamide gel electrophoresis. Result revealed presence of 3 (range 15.2–40.3 kDa), 13 (9.3–121.2 kDa), 14 (9.3–169.3 kDa), and 15 (8.0–169.3 kDa) polypeptides in McAg, ImIAg, ImRAg, and AAg, respectively. With an aim to identify a suitable immunodiagnostic antigen for early diagnosis of amphistomosis, the IgG antibody response to various developmental stage antigens in goats experimentally infected with metacercariae of P. epiclitum was evaluated by ELISA. The highest OD values were recorded with ImIAg which ranged between 0.23 and 0.55 with a significant increase from the 2nd week till 8th week of infection with a peak at 6th week. The analysis of statistical significance using a one-way analysis of variance with multiple pair wise comparisons revealed that IgG response was significantly higher with all antigens (P < 0.01) except McAg (P > 0.05) with a maximum mean difference of 0.1838 in comparison to control with ImIAg, thus, indicating that ImIAg which could be further exploited for its potential is a candidate for immunodiagnostic antigen for early diagnosis of amphistomosis.

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“…The extra positivity observed in this group might be due to prepatent infection or cross reactivity with other closely related helminths parasites (Maji et al, 1999;Yadav et al, 2003;Salib et al, 2015). The observed specificity of the group PN in the plate-ELISA in the goats was 89.83 (PeSAg) and 91.53% (PeESAg) and in sheep was 89.23 (PeSAg) and 93.85% (PeESAg).…”

Section: Polypeptide Profile Of Antigensmentioning

confidence: 92%

“…The variation observed in polypeptide profile, by each worker, could be attributed to the method of antigens preparation. Purification of P. epiclitum functional antigens by specific methods is expected to remove cross reacting components, which if present are liable to cross react and elicit false results in ELISA (Maji et al, 1999;Yadav et al, 2003;Pal and Dasgupta, 2007;Kaur et al, 2013). The phosphoryl-choline epitope is the main element of crossreaction, an important conserved epitope of trematodes (Sloan et al, 1991).…”

Section: Polypeptide Profile Of Antigensmentioning

confidence: 99%

Immunodiagnostic potency of homologous antigens for natural Paramphistomum epiclitum infection in small ruminants in plate and paper enzyme linked immunosorbent assay

Kumar

Jadav

Das

et al. 2017

IJAR

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The objective of the present work was to standardize and evaluate indirect plate and dot-enzyme linked immunosorbent assay (ELISA) using purified Paramphistomum epiclitum homologous antigens in the small ruminants. Electrophoretic separation of somatic antigen (PeSAg) in reducing condition on 15% polyacrylamide gel resolved into 16 proteins of the molecular weight ranging from 14 -100 kDa. Two step ethanolic precipitation of supernatant of in-vitro culture of the fluke yielded P. epiclitum excretory-secretory antigen (PeESAg) of molecular weight 28 kDa. The animals (Goats=123; Sheep=91) were broadly kept into post-mortem and faecal examined groups. At many occasion the PeSAg found to cross reacts with other helminths parasites thus minimizing the specificity of the tests and antigens. There was no any direct correlation between the parasites load and ELISA reactivity pattern. The noted prevalence rate after combining the results of postmortem examination and PeESAg based ELISA (plate and paper/ dot) was 30.08% (37/123) in goats and 28.57% (26/91) in sheep. While using PeESAg, the calculated overall sensitivity% was 92.86 (goats)/ 100 (sheep) in both plate and dot-ELISA, specificity% was 91.58 (goats)/ 91.55 (sheep) in plate ELISA while 88.42 (goats)/ 92.96 (sheep) in dot-ELISA, positive predictive value% was 76.47 (goats)/ 76.92 (sheep) in plate ELISA while 70.27 (goats)/ 80 (sheep) in dot-ELISA and negative predictive value% was 97.75 (goats)/ 100 (sheep) in plate ELISA while 97.67 (goats)/ 100 (sheep) in dot-ELISA, these values were optimum for the field sera sample so the tests and PeESAg can be recommended for the detection P. epiclitum infection in the small ruminants.

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Cross-antigenicity Between Ruminal and Hepatic Paramphistomes and Liver Flukes of Buffalo Origin

Cited by 5 publications

References 3 publications

Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum

Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum

Evaluation of Antibody Response to Various Developmental Stage Specific Somatic Antigens ofParamphistomum epiclitumin Goats

Immunodiagnostic potency of homologous antigens for natural Paramphistomum epiclitum infection in small ruminants in plate and paper enzyme linked immunosorbent assay

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