Cross-amplification of microsatellites from the Atherinopsidae Odontesthes perugiae and Odontesthes argentinensis to Chilean silversides of the genus Odontesthes and Basilichthys

Muñóz, P. Gomis; Quezada‐Romegialli, Claudio; Vila, Irma; Véliz, David

doi:10.4067/s0717-65382011000200007

Cited by 7 publications

(8 citation statements)

References 12 publications

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“…These results are consistent with those of Muñoz et al (2011), who used the same loci with a larger sample size. MICROCHECKER did not reveal null alleles, and homozygote excess was detected in only two populations for one locus (Table S2).…”

Section: Descriptive Statistics and Population Structuresupporting

confidence: 90%

Genetic diversity and origin of a fish population recently colonizing a reservoir: The case ofBasilichthys microlepidotus, central Chile

et al. 2022

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Freshwater ecosystems are among those most affected by human activity. Constructing artificial reservoirs can change freshwater biodiversity, thereby impacting genetic diversity (GD), which is crucial to population-level adaptation. The Maipo River basin, inhabited by the endemic silverside Basilichthys microlepidotus, is one of Chile's most impacted freshwater systems. In this basin, the species is divided into five populations. Recently, a new population was discovered in the reservoir Laguna Esmeralda (LE). We hypothesized that LE silversides possess less GD than other populations and originated from a single basin population. Our evidence shows that the LE is a genetically isolated population with evidence of low genetic variation, but not showing signals of bottleneck or significant reduction in GD. Results consistently identified two sites as unlikely to be the origin of the LE silverside population, suggesting that it was established by the immigration of many individuals from the other three populations.

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Section: Descriptive Statistics and Population Structuresupporting

confidence: 90%

Genetic diversity and origin of a fish population recently colonizing a reservoir: The case ofBasilichthys microlepidotus, central Chile

et al. 2022

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“…A total of 10 microsatellite loci were screened for the silverside B. microlepidotus : five loci (OD02, OD07, OD09, OD38 and OD39) described by Beheregaray and Sunnucks (2000) and five loci (OB01, OB19, OB44, 0B59 and OB71) described by Koshimizu et al (2009). These microsatellites were amplified following the protocol described by Muñoz, Quezada‐Romegialli, and Véliz (2011). The 10 microsatellite loci (TR01, TR04, TR06, TR10, TR11, TR14, TR17, TR21, TR22 and TR24) described by Muñoz‐Rojas, Quezada‐Romegialli, and Véliz (2012) were screened for the catfish T. areolatus .…”

Section: Methodsmentioning

confidence: 99%

“…DNA was obtained using the protocol described by Aljanabi and Martinez (1997) Beheregaray and Sunnucks (2000) and five loci (OB01, OB19, OB44, 0B59 and OB71) described by Koshimizu et al (2009). These microsatellites were amplified following the protocol described by Muñoz, Quezada-Romegialli, and Véliz (2011).…”

Section: Genetic Analysismentioning

confidence: 99%

Dammed river: Short‐ and long‐term consequences for fish species inhabiting a river in a Mediterranean climate in central Chile

Vega‐Retter

Muñoz‐Rojas

Rojas‐Hernández

et al. 2020

Aquatic Conservation

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1. The presence of a dam disturbs river flow, which in turn directly affects the communities and evolutionary potential of riverine species. To detect the ecological effects of a dam on genetic diversity, genetic structure, and their progress in time, two riverine fishes living upstream and downstream of an irrigation reservoir were studied at two periods after its construction in 2004 in central Chile. 2. Samples of the Neotropical silverside Basilichthys microlepidotus and the pencil catfish Trichomycterus areolatus were obtained 2 and 7 years after the Corrales Dam was built. The microsatellite DNA variability of both species upstream and downstream of the dam was analysed. 3. Fish analysed 2 years after dam construction did not show genetic differences between upstream and downstream populations; however, fish obtained 7 years after dam construction showed differences between populations above and below the dam and differences from individuals collected 5 years earlier. 4. The current effective population sizes of both species were smaller in samples obtained upstream than in samples obtained downstream. Simulations showed a migration equal to zero as most probable after reservoir construction, suggesting that the dam is an impermeable barrier to the movement of individuals of these species. These results showed that population fragmentation in time could be related to the barrier imposed by the dam. 5. In a scenario of no new contact between populations located upstream and downstream of the dam, the simulation predicts a reduction of genetic diversity ranging from 3.98 to 8.09% over the next 60 years. 6. Analyses suggest that the Corrales Reservoir may be affecting the evolutionary potential of the populations upstream and downstream from the dam.

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“…Microsatellites were amplified by PCR in a volume of 10 μL, including 1.3 μL 10x PCR buffer (Invitrogen), 0.5 μL MgCl2 (50 mM) (Invitrogen), 0.5 μL of direct and inverse primers (50 ng μL -1 ) (Applied Biosystems), 2.4 μL dNTPs (2.5 mM) (Invtrogen), 4.68 μL H2O and 0.12 μL Taq polymerase (Invitrogen), to which 1.5 μL DNA (50 ng μL -1 ) were added. The PCR touchdown method of Beheregaray & Sunnucks (2000) was used to amplify microsatellites Odont02, Odont07, Odont09 and Odont38, with the modifications of Muñoz et al (2011). Loci Obo19TUF, Obo59TUF and Obo71TUF were amplified using the protocol of Genetic analyses, genetic diversity and population structure The allelic matrix was generated with GeneMarker software (SoftGenetics Inc).…”

Section: Methodsmentioning

confidence: 99%

Genetic population structure and evidence of genetic homogeneity in populations of the Argentinian silverside Odontesthes bonariensis (Teleostei: Atherinopsidae) inhabiting central and northwestern Argentina

Valencia

Véliz

Tombari

et al. 2017

lajar

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ABSTRACT. The study of species in their native geographic ranges is key to understanding how human activity has influenced spatial fragmentation or species homogenization. The Argentinian silverside Odontesthes bonariensis, of interest for aquiculture and sport fishing, is a relevant subject of study. The species has been introduced in a number of countries and re-introduced in some areas of Argentina with unknown effects. The objectives of this study were to determine the population structure, genetic diversity (GD) and effective population sizes (Ne) of O. bonariensis in Argentina. Six microsatellite loci were amplified in individuals collected from four water bodies affected by commercial and sport fishing: Cabra Corral Reservoir (CC), Chascomús Lake (CH), Chasicó Lake (LCH) and the Río de la Plata (RLP). Three genetic groups were detected: one in CC, one in RLP and the last inhabiting CH and LCH. Interestingly, CH and LCH are located 768 km apart, but showed no difference in allele frequencies; suggesting the introduction of individuals from CH into LCH. The largest allele richness, GD and Ne were found in RLP indicating that the largest population of O. bonariensis may be found in this area. Current Ne were lower than historical Ne in all areas, suggesting a change in the GD over time. This study provides information on the genetic structure and genetic diversity of O. bonariensis across its native distribution and over time, demonstrating the first evidence of a possible genetic homogenization in this species probably linked to human activities.

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Cross-amplification of microsatellites from the Atherinopsidae Odontesthes perugiae and Odontesthes argentinensis to Chilean silversides of the genus Odontesthes and Basilichthys

Cited by 7 publications

References 12 publications

Genetic diversity and origin of a fish population recently colonizing a reservoir: The case ofBasilichthys microlepidotus, central Chile

Genetic diversity and origin of a fish population recently colonizing a reservoir: The case ofBasilichthys microlepidotus, central Chile

Dammed river: Short‐ and long‐term consequences for fish species inhabiting a river in a Mediterranean climate in central Chile

Genetic population structure and evidence of genetic homogeneity in populations of the Argentinian silverside Odontesthes bonariensis (Teleostei: Atherinopsidae) inhabiting central and northwestern Argentina

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