Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum

Pinheiro, Fábio; Palma‐Silva, Clarisse; Barros, Fábio de; Cozzolino, Salvatore

doi:10.1590/s1415-47572009005000037

Cited by 14 publications

(16 citation statements)

References 7 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(2007). Amplification, sequencing, primer design and the screening of polymorphisms were carried out following Pinheiro et al. (2009).…”

Section: Methodsmentioning

confidence: 99%

“…Primer pairs were designed by sequencing noncoding regions using the universal primers described by Taberlet et al (1991), Demesure et al (1995), Sang et al (1997), Small et al (1998) and Shaw et al (2007). Amplification, sequencing, primer design and the screening of polymorphisms were carried out following Pinheiro et al (2009). The characteristics and sequences of the primers designed for each plastid SSR marker are reported in Table S1 (Supporting information).…”

Section: Identification Of Plastid Dna Markersmentioning

confidence: 99%

See 1 more Smart Citation

Sympatric bromeliad species (Pitcairnia spp.) facilitate tests of mechanisms involved in species cohesion and reproductive isolation in Neotropical inselbergs

Wendt²,

et al. 2011

View full text Add to dashboard Cite

The roles of intra-and interspecific gene flow in speciation and species evolution are topics of great current interest in molecular ecology and evolutionary biology. Recent modelling studies call for new empirical data to test hypotheses arising from the recent shift from a 'whole-genome reproductive isolation' view to a 'genic' view of species and speciation. Particularly scarce (and thus of particular interest) are molecular genetic data on recently radiated, naturally hybridizing species in strongly structured and species-rich environments. Here, we studied four sympatric plant species (Pitcairnia spp.; Bromeliaceae) adapted to Neotropical inselbergs (isolated outcrops resembling habitat 'islands' in tropical rainforests) using nuclear and plastid DNA. Patterns of plastid DNA haplotype sharing and nuclear genomic admixture suggest the presence of both, incomplete lineage sorting and interspecific gene flow over extended periods of time. Integrity and cohesion of inselberg species of Pitcairnia are maintained despite introgression and in the face of extremely low within-species migration rates (N e m < 1 migrant per generation). Cross-evaluation of our genetic data against published pollination experiments indicate that species integrity is maintained by the simultaneous action of multiple prezygotic barriers, including flowering phenology, pollinator isolation and divergent mating systems. Postzygotic Bateson-Dobzhansky-Muller incompatibilities appear to contribute to isolation, as suggested by asymmetric introgression rates of single loci. Our results suggest that incomplete lineage sorting, hybridization and introgression form integral aspects of adaptive radiation in Neotropical inselberg 'archipelagos'. Inselbergs with multiple closely related co-occurring species should be of special interest to students of speciation in mountain systems, and to ongoing conservation programmes in the Atlantic Rainforest biodiversity hotspot.

show abstract

“…(2007). Amplification, sequencing, primer design and the screening of polymorphisms were carried out following Pinheiro et al. (2009).…”

Section: Methodsmentioning

confidence: 99%

Section: Identification Of Plastid Dna Markersmentioning

confidence: 99%

Sympatric bromeliad species (Pitcairnia spp.) facilitate tests of mechanisms involved in species cohesion and reproductive isolation in Neotropical inselbergs

Wendt²,

et al. 2011

View full text Add to dashboard Cite

show abstract

“…There were no amplifications in three loci for C. loddigesii (Cw07, Cw08 and Cw09), showing 62.5% transferability, and in two loci for C. nobilior (Cw07 and Cw09), showing 75% transferability. Many studies have also demonstrated the transferability of microsatellite loci to other species of the same genus (Swarts et al, 2007;Pinheiro et al, 2009), including the genus Cattleya (Novello et al, 2013), and even to species of other genera (Cortés-Palomec et al, 2008;Rai et al, 2013). Such studies that are important for the success in the conservation of rare, threatened or endangered species are lacking for most tropical orchid species (Frankham et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Microsatellite markers for Cattleya walkeriana Gardner, an endangered tropical orchid species

Tambarussi

Menezes

Ibanes³

et al. 2016

Plant Genet. Resour.

View full text Add to dashboard Cite

Cattleya walkeriana, one of the most improved Brazilian Cattleyas, is a popular tropical orchid endemic from Brazil and currently endangered. In the present study, for the first time microsatellite markers were developed for C. walkeriana and their transferability was tested for the species C. loddigesii and C. nobilior. The markers were used for genotyping 26 C. walkeriana specimens from different growers and from different levels of improvement. The transferability was successful, with five polymorphic loci transferred to C. loddigesii and six polymorphic loci to C. nobilior. Eight loci were polymorphic, revealing a maximum of two to ten alleles per locus in C. walkeriana and two to four and two to five in C. loddigesii and C. nobilior, respectively. There was no significant linkage disequilibrium in the studied loci. For C. walkeriana, the observed and expected heterozygosities ranged from 0 to 0.963 and from 0.138 to 0.841, respectively. These markers identified polymorphisms and may be used to study the genetic diversity, gene flow or hybridization of these species.

show abstract

“…SSR cross-amplification is a potential source of variation for evolutionary and taxonomic studies, with the SSR transfer rate corresponding to taxonomic distance (Ellis and Burke, 2007;Pinheiro et al, 2009). This approach may be helpful for studying the Dalbergia clade, in which Aeschynomene genus is included.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization

et al. 2014

View full text Add to dashboard Cite

ABSTRACT. Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are desirable for improving breeding programs for A. falcata. Based on transferability and in situ localization, these microsatellite sequences can be applied as chromosome markers in the genus Aeschynomene and closely related genera. Here, we report the first microsatellite library developed for this genus; 11 microsatellites were characterized, with observed and Microsatellite loci in Aeschynomene falcata expected heterozygosities ranging from 0.0000 to 0.7143 and from 0.1287 to 0.8360, respectively. Polymorphic information content varied from 0.1167 to 0.7786. The departure from Hardy-Weinberg equilibrium may have resulted from frequent autogamy, which is characteristic of A. falcata. Of the 11 microsatellites, 9 loci were cross-amplified in A. brevipes and A. paniculata and 7 in Dalbergia nigra and Machaerium vestitum. Five of these 7 cross-amplified microsatellites were applied as probes during the in situ hybridization assay and 2 showed clear signals on A. falcata chromosomes, ensuring their viability as chromosome markers.

show abstract

Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum

Cited by 14 publications

References 7 publications

Sympatric bromeliad species (Pitcairnia spp.) facilitate tests of mechanisms involved in species cohesion and reproductive isolation in Neotropical inselbergs

Sympatric bromeliad species (Pitcairnia spp.) facilitate tests of mechanisms involved in species cohesion and reproductive isolation in Neotropical inselbergs

Microsatellite markers for Cattleya walkeriana Gardner, an endangered tropical orchid species

Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization

Contact Info

Product

Resources

About