Crop reproductive meristems in the genomic era: a brief overview

Caselli, Francesca; Zanarello, Fabio; Kater, Martin M.; Battaglia, Raffaella; Gregis, Veronica

doi:10.1042/bst20190441

Cited by 3 publications

(4 citation statements)

References 117 publications

(118 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Paraplast‐embedded tissues were sliced on an RM2155 microtome (Leica, https://www.leica-microsystems.com) at a thickness of 8 μm and hybridized as described by Caselli et al. (2020), with minor modifications. Immunodetection was carried out with anti‐digoxigenin‐AP Fab fragment (Roche) and BCIP‐NBT color development substrate (Promega), as specified by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

“…Digoxigenin-labeled antisense and sense RNA probes were transcribed and labeled with pGEMâ-T Easy with T7/SP6 RNA polymerase (Promega), according to the manufacturer's instructions and using the DIG RNA labelling mix (Roche, https://www.roche.com). Paraplast-embedded tissues were sliced on an RM2155 microtome (Leica, https://www.leicamicrosystems.com) at a thickness of 8 lm and hybridized as described by Caselli et al (2020), with minor modifications. Immunodetection was carried out with anti-digoxigenin-AP Fab fragment (Roche) and BCIP-NBT color development substrate (Promega), as specified by the manufacturer.…”

Section: Tissue Fixation and In Situ Hybridizationmentioning

confidence: 99%

“…Oryza sativa , commonly known as rice, develops a complex and determinate inflorescence, named panicle (Bommert et al., 2005; Han et al., 2014). Its architecture is established during early stages of rice reproductive development, and it depends on the activity of different meristem types (Caselli et al., 2020; Tanaka et al., 2013). During the floral transition, the rice shoot apical meristem (SAM) becomes an IM, also called a rachis meristem.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The ALOG family members OsG1L1 and OsG1L2 regulate inflorescence branching in rice

et al. 2023

Self Cite

View full text Add to dashboard Cite

SUMMARY The architecture of the rice inflorescence is an important determinant of crop yield. The length of the inflorescence and the number of branches are among the key factors determining the number of spikelets, and thus grains, that a plant will develop. In particular, the timing of the identity transition from indeterminate branch meristem to determinate spikelet meristem governs the complexity of the inflorescence. In this context, the ALOG gene TAWAWA1 (TAW1) has been shown to delay the transition to determinate spikelet development in Oryza sativa (rice). Recently, by combining precise laser microdissection of inflorescence meristems with RNA‐seq, we observed that two ALOG genes, OsG1‐like 1 (OsG1L1) and OsG1L2, have expression profiles similar to that of TAW1. Here, we report that osg1l1 and osg1l2 loss‐of‐function CRISPR mutants have similar phenotypes to the phenotype of the previously published taw1 mutant, suggesting that these genes might act on related pathways during inflorescence development. Transcriptome analysis of the osg1l2 mutant suggested interactions of OsG1L2 with other known inflorescence architecture regulators and the data sets were used for the construction of a gene regulatory network (GRN), proposing interactions among genes potentially involved in controlling inflorescence development in rice. In this GRN, we selected the homeodomain‐leucine zipper transcription factor encoding the gene OsHOX14 for further characterization. The spatiotemporal expression profiling and phenotypical analysis of CRISPR loss‐of‐function mutants of OsHOX14 suggests that the proposed GRN indeed serves as a valuable resource for the identification of new proteins involved in rice inflorescence development.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Tissue Fixation and In Situ Hybridizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The ALOG family members OsG1L1 and OsG1L2 regulate inflorescence branching in rice

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Oryza sativa, commonly known as rice, develops a complex and determinate inflorescence, named panicle (Bommert et al, 2005;Han et al, 2014). Its architecture is established during early stages of rice reproductive development and it depends on the activity of different meristem types (Tanaka et al, 2013;Caselli et al, 2020). During the floral transition, the rice Shoot Apical Meristem (SAM) becomes Inflorescence Meristem (IM), also called rachis meristem.…”

Section: Introductionmentioning

confidence: 99%

The ALOG family membersOsG1L1andOsG1L2regulate inflorescence branching in rice

Franchini

Beretta

Din

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

The architecture of the rice inflorescence is an important determinant of seed yield. The length of the inflorescence and the number of branches are among the key factors determining the amount of spikelets, and thus seeds, that will develop. Especially the timing of the identity transition from indeterminate branch meristem to determinate spikelet meristem regulates the complexity of the inflorescence. In this context, the ALOG gene TAWAWA1 (TAW1) has been shown to delay the transition to determinate spikelet development in rice. Recently, by combining precise laser microdissection of inflorescence meristems with RNA-seq we observed that two ALOG genes, Oryza sativa OsG1-like 1 (OsG1L1) and OsG1L2, have an expression profile similar to TAW1. Here we report that osg1l1 and osg1l2 loss-of-function CRISPR mutants have similar phenotypes as the taw1 mutant, suggesting that these genes might act on related pathways during inflorescence development. Transcriptome analysis of the osg1l2 mutant suggested interactions of OsG1L2 with other known inflorescence architecture regulators and the datasets were also used for the construction of a gene regulatory network (GRN) proposing interactions between genes potentially involved in controlling inflorescence development in rice. The spatio-temporal expression profiling and phenotypical analysis of CRISPR loss-of-function mutants of the homeodomain-leucine zipper transcription factor gene OsHOX14 suggest that the proposed GRN indeed serves as a valuable resource for the identification of new players involved in rice inflorescence development.

show abstract