Exploration and Validation of the Potential Downstream Genes Underlying ipa1-2D Locus for Rice Panicle Branching

Zhang, Lin; Xie, Dong; Bian, Zhong; Zou, Yiting; Zhou, Han; Cai, Wenlu; Yeilaghi, Hadi; Fan, Xiaolei; Zhang, Changquan; Liu, Qiaoquan

doi:10.32604/phyton.2021.015229

Cited by 1 publication

(1 citation statement)

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“…IPA1 was regarded as a new “green revolution” gene for its significant potential in enhancing grain yield 34 , and its natural elite gain-of-function alleles, especially the ipa1-2D , have made great contributions in breeding new super hybrid rice varieties 22 , 35 , 36 . The ipa1-2D contains a 3,137-bp tandem repeat in the upstream of IPA1 , which can elevate the expression of IPA1 and increase the stem thickness, grain number, and yield.…”

Section: Resultsmentioning

confidence: 99%

Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice

et al. 2022

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Chilling is a major abiotic stress harming rice development and productivity. The C-REPEAT BINDING FACTOR (CBF)-dependent transcriptional regulatory pathway plays a central role in cold stress and acclimation in Arabidopsis. In rice, several genes have been reported in conferring chilling tolerance, however, the chilling signaling in rice remains largely unknown. Here, we report the chilling-induced OSMOTIC STRESS/ABA-ACTIVATED PROTEIN KINASE 6 (OsSAPK6)-IDEAL PLANT ARCHITECTURE 1 (IPA1)-OsCBF3 signal pathway in rice. Under chilling stress, OsSAPK6 could phosphorylate IPA1 and increase its stability. In turn, IPA1 could directly bind to the GTAC motif on the OsCBF3 promoter to elevate its expression. Genetic evidence showed that OsSAPK6, IPA1 and OsCBF3 were all positive regulators of rice chilling tolerance. The function of OsSAPK6 in chilling tolerance depended on IPA1, and overexpression of OsCBF3 could rescue the chilling-sensitive phenotype of ipa1 loss-of-function mutant. Moreover, the natural gain-of-function allele ipa1-2D could simultaneously enhance seedling chilling tolerance and increase grain yield. Taken together, our results revealed a chilling-induced OsSAPK6-IPA1-OsCBF signal cascade in rice, which shed new lights on chilling stress-tolerant rice breeding.

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