Crocin, a carotenoid, suppresses spindle microtubule dynamics and activates the mitotic checkpoint by binding to tubulin

Sawant, Avishkar V.; Srivastava, Shalini; Prassanawar, Shweta Shyam; Bhattacharyya, Bhabatarak; Panda, Dulal

doi:10.1016/j.bcp.2019.01.023

Cited by 14 publications

(16 citation statements)

References 51 publications

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“…V X and B X are the respective concentrations of vancomycin and BT‐benzo‐29 that produce the same effect when used alone. V X and B X are calculated using the median‐effect equation as follows:

V_{X} = V_{m} {[\frac{f_{normala}}{f_{normalu}}]}^{1 / m},

where V m is the median effective dose of vancomycin, f a and f u are the fractions of cell affected and unaffected, respectively, and m is the slope. The median effective dose, V m , was calculated by plotting a median effect graph, where X = log[ V ], Y = log ( f a / f u ), and V m = 10 –( Y −intercept)/ m .…”

Section: Methodsmentioning

confidence: 99%

“…The combinatorial effect of BT‐benzo‐29 (0.5 and 1 μM) with PMB (8 and 13 μM) was determined as described earlier. The combinatorial effect of BT‐benzo‐29 (0.5 and 1 μM) with PMB (8 and 13 μM) was determined by colony count assay, and CI was calculated as described previously . The experiment was performed three times.…”

Section: Methodsmentioning

confidence: 99%

“…V X and B X are the respective concentrations of vancomycin and BT-benzo-29 that produce the same effect when used alone. V X and B X are calculated using the median-effect equation 34,35 as follows:…”

Section: Pl2084 Cellsmentioning

confidence: 99%

“…The combinatorial effect of BT-benzo-29 (0.5 and 1 μM) with PMB (8 and 13 μM) was determined by colony count assay, and CI was calculated as described previously. [33][34][35] The experiment was performed three times.…”

Section: Pl2084 Cellsmentioning

confidence: 99%

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Mechanistic insight into the effect of BT‐benzo‐29 on the Z‐ring in Bacillus subtilis

et al. 2020

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The assembly and disassembly of FtsZ play an essential role in bacterial cell division. Using single‐cell imaging, we report that short exposure to BT‐benzo‐29 inhibits Z‐ring formation in live Bacillus subtilis cells. Fluorescence recovery after photobleaching of the Z‐ring in live bacteria demonstrated that BT‐benzo‐29 strongly suppressed the assembly dynamics of FtsZ in the Z‐ring. Furthermore, B. subtilis cells expressing V275A‐FtsZ resisted the antibacterial activity of BT‐benzo‐29 providing evidence that BT‐benzo‐29 inhibits bacterial proliferation by targeting FtsZ. In addition, a brief (8 min) exposure of BT‐benzo‐29 destroyed the Z‐ring without perturbing the localization of a late cell division protein, DivIVA, the nucleoid segregation, and membrane permeability. BT‐benzo‐29, when used in combination with vancomycin and polymyxin B (PMB), produced a much stronger inhibitory effect on Bacillus subtilis and Escherichia coli cells, respectively. The combination index of BT‐benzo‐29 with vancomycin and PMB was determined to be <1, suggesting that BT‐benzo‐29 exhibits synergistic inhibitory effects on bacterial proliferation when used along with these antibiotics.

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V_{X} = V_{m} {[\frac{f_{normala}}{f_{normalu}}]}^{1 / m},

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Pl2084 Cellsmentioning

confidence: 99%

Section: Pl2084 Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Mechanistic insight into the effect of BT‐benzo‐29 on the Z‐ring in Bacillus subtilis

et al. 2020

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“…Recently, it has been reported that vinblastine and crocin share their binding sites on tubulin (Sawant et al, 2019;Hire et al;. Therefore, we examined whether 2l could inhibit the binding of crocin to tubulin.…”

Section: Competitive Inhibition With Vinblastine and Crocinmentioning

confidence: 99%