Critical Periods in the Development of Mechanoreceptors

Zelená, Jiřina; Jirmanová, Isa

doi:10.1007/978-1-4899-0812-4_2

Cited by 25 publications

(48 citation statements)

References 17 publications

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“…In these patients p75 IR was at residual levels in Meissner corpuscles and was absent in the inner-core cells of Pacinian corpuscles. This is consistent with ultrastructural data reporting atrophy of corpuscular Schwann-related cells after nerve transection (Dellon et al, 1975;Kosima et al, 1992;Zelena, 1994). Furthermore, our data suggest that these corpuscles are not reinnervated (Wada, 1989).…”

Section: Nerves and Sensory Corpusclessupporting

confidence: 93%

“…In the Pacinian corpuscles, the central axon is surrounded by the inner core, formed of two hemi-cylinders of packed flattened processes of lamellar cells. The inner core in turn is surrounded by concentric lamellar layers separated by fluid-filled spaces which can be divided into the outer core (the innermost lamellae) and the capsule (for details see Munger and Ide, 1988;Zelena, 1994). The three main constituents of sensory corpuscles (i.e., the central axon, the lamellar cells and inner core, and the outer core and capsule) were easily identified because they expressed specific IR for different antigens.…”

Section: Resultsmentioning

confidence: 99%

“…The main structural (Halata, 1975;Iggo and Andres, 1982;Malinovsky, 1986;Munger and Ide, 1988;Zelena, 1994), biochemical (see Dubový, 1996), and immunohistochemical (see Vega et al, 1996b) characteristics of the cells forming sensory corpuscles have been extensively analyzed. Studies on corpuscular changes following injuries of the nerves supplying them are also abundant and have been so far addressed mainly to structural (for references see Zelena, 1994) and histochemical (see Dubový and Aldskogius, 1996) aspects.…”

Section: Discussionmentioning

confidence: 99%

“…Studies on corpuscular changes following injuries of the nerves supplying them are also abundant and have been so far addressed mainly to structural (for references see Zelena, 1994) and histochemical (see Dubový and Aldskogius, 1996) aspects. More recently (for references see Má rquez et al, 1997) this topic has been addressed by means of immunohistochemical techniques.…”

Section: Discussionmentioning

confidence: 99%

“…Peripheral nerve lesions cause a series of morphological (see Zelena, 1994) and biochemical Ramieri et al, 1995;Dubový and Aldskogius, 1996;Már-quez et al, 1997) modifications in cutaneous sensory corpuscles, including upregulation of p75 (Yamamoto et al, 1992). Nevertheless, whether injury of the central (spinal cord transection) or peripheral (entrapment or section graft) processes of primary neurons modify the expression of neurotrophin receptors in human cutaneous sensory corpuscles has not been studied.…”

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p75 and TrkA neurotrophin receptors in human skin after spinal cord and peripheral nerve injury, with special reference to sensory corpuscles

et al. 1998

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Human skin, including nerves and sensory corpuscles, displays immunoreactivity (IR) for low-(p75) and high-affinity (TrkA-like) receptors for nerve growth factor (NGF), the best characterized member of the family of neurotrophins. This study was designed to analyze the changes induced by spinal cord and peripheral nerve injuries in the expression of neurotrophin receptors in digital skin, with special reference to nerves and sensory corpuscles.Skin biopsy samples were obtained from 1) the hand and toes of normal subjects, 2) below the level of the lesion of patients with spinal cord injury affecting dorsal and lateral funiculi, 3) the cutaneous territory of entrapped peripheral nerves (median and ulnar nerves), and 4) the cutaneous territory of sectioned and grafted nerves (median nerve). The pieces were formalinfixed and paraffin-embedded, cut in serial sections, and processed for immunohistochemistry using antibodies against human p75 and TrkA proteins. The percentage of sensory corpuscles displaying IR for p75 and TrkA-like, as well as the intensity of IR developed within them, was assessed using quantitative image analysis.Spinal cord severance causes a decrease in p75 IR in Meissner and Pacinian corpuscles, whereas TrkA-like IR did not vary. In other nonnervous tissues (i.e., epidermis, sweat glands), both p75 and TrkA-like IR was diminished or even absent. Similar but more severe changes were encountered in the skin from the territory of entrapped nerves. Finally, in subjects with sectioned-grafted nerves, p75 IR was found close to controls in nerves, reduced in Meissner corpuscles, and absent in the inner core of the Pacinian ones; TrkA-like IR was in the perineurium, a small percentage of Meissner corpuscles (about 7%), and the outer core and capsule of the Pacinan corpuscles. In the nonnervous tissues, p75 IR was practically absent, whereas TrkA-like IR did not change. No changes in the expression of neurotrophin receptors were observed in Merkel cells of the different groups.Present results show the following: 1) expression of nerve p75 IR in human cutaneous sensory corpuscles is sensitive to central deafferentation, to blockade or difficulty in axonal transport, and to disruption of axonal

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Section: Nerves and Sensory Corpusclessupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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p75 and TrkA neurotrophin receptors in human skin after spinal cord and peripheral nerve injury, with special reference to sensory corpuscles

et al. 1998

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Degeneration and regeneration of cutaneous sensory nerve formations

Dubový¹,

Aldskogius

1996

Microsc. Res. Tech.

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S100α and S100β proteins in human cutaneous sensory corpuscles: Effects of nerve and spinal cord injury

et al. 1998

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S100 protein in the vertebrate peripheral nervous system consists of homo- or heterodimers of S100alpha and S100beta proteins, the first predominating in neurons and the second in glial cells. Recently, however, occurrence of S100beta protein in neurons has been reported. The expression of S100 protein by Schwann cells, as well as their derivatives in sensory corpuscles, depends on the sensory axon (i.e., the Schwann cell-axon contact). The present study analyzed the distribution of S100alpha and S100beta proteins in human cutaneous sensory corpuscles and the effects of peripheral or central sensory axon severance in the expression of these proteins. Simple or double immunohistochemistry was carried out using a panel of antibodies against S100alpha, S100beta or S100alpha+beta proteins, and the sections were examined by light or laser confocal scanning microscopy. Skin samples were obtained from normal subjects and patients with spinal cord injury, nerve entrapment, and nerve sections plus graft. The lamellar cells of Meissner corpuscles as well as the inner-core lamellae of the Pacinian corpuscles displayed strong immunoreactivity (IR) for all antigens examined, the most intense labeling being obtained for S100beta protein. The pattern of immunostaining was unchanged after spinal cord injury, whereas the number of stained corpuscles as well as the intensity of IR for each antigen decreased in cutaneous sensory corpuscles after nerve injury, both entrapment and section plus graft. No evidence was found of axonal labeling. The present results provide evidence that Schwann-related cells in human cutaneous sensory corpuscles contain both S100alpha and S100beta and that the expression of these proteins is dependent on the functional and structural integrity of sensory fibers.

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Critical Periods in the Development of Mechanoreceptors

Cited by 25 publications

References 17 publications

p75 and TrkA neurotrophin receptors in human skin after spinal cord and peripheral nerve injury, with special reference to sensory corpuscles

p75 and TrkA neurotrophin receptors in human skin after spinal cord and peripheral nerve injury, with special reference to sensory corpuscles

Degeneration and regeneration of cutaneous sensory nerve formations

S100α and S100β proteins in human cutaneous sensory corpuscles: Effects of nerve and spinal cord injury

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