Critical overview on the application of sensors and biosensors for clinical analysis

Justino, Celine I.L.; Duarte, Armando C.; Rocha-Santos, Teresa

doi:10.1016/j.trac.2016.04.004

Cited by 117 publications

(69 citation statements)

References 156 publications

(369 reference statements)

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“…Improving the analytical performance of sensors is essential for producing prototypes and in turn subsequent commercialization of these systems, for example, in environmental monitoring, food safety or clinical analysis. One of the ways of such enhancement is by improving the associated figures of merit such as sensitivity . Nanotechnology has been applied to enhance the performance of electrochemical biosensors.…”

Section: Physically Small Sensing Devicesmentioning

confidence: 99%

Recent Advances in Biosensing for Neurotransmitters and Disease Biomarkers using Microelectrodes

2017

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This Minireview focuses on recent advances in the applications of microelectrodes to detect and monitor targeted analytes in bioelectrochemical processes. Notably, these processes are electrochemically driven reactions that involve the detection of targets from the biological realm. Wide‐ranging applications of electrochemical sensors have been reported in the last few decades in various research fields, owing to favorable attributes such as high selectivity and sensitivity, rapid analysis, simplicity, easy fabrication, and cost effectiveness. Accordingly, in this Minireview, we explore recent advances in bioelectrochemistry based on small detection probes or structures modified for a variety of analytes, exploiting multi‐approach advantages of enhanced electrochemical detection surface or targeted analyte pursuit. The target analytes included in this Minireview are neurotransmitters and disease biomarkers detected using enzymatic and non‐enzymatic electrode modifications.

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Section: Physically Small Sensing Devicesmentioning

confidence: 99%

Recent Advances in Biosensing for Neurotransmitters and Disease Biomarkers using Microelectrodes

2017

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“…Bio-sensors play an essential role in medical diagnostics, environmental monitoring and food safety analysis [1,2]. Nucleic acid assays are key targets in biosensing that enables viral load monitoring and disease diagnosis [3,4].…”

Section: Introductionmentioning

confidence: 99%

Pixelated colorimetric nucleic acid assay

Aydın

Cheema

Ammanath

et al. 2020

Talanta

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A B S T R A C TConjugated polyelectrolytes (CPEs) have been widely used as reporters in colorimetric assays targeting nucleic acids. CPEs provide naked eye detection possibility by their superior optical properties however, as concentration of target analytes decrease, trace amounts of nucleic acid typically yield colorimetric responses that are not readily perceivable by naked eye. Herein, we report a pixelated analysis approach for correlating colorimetric responses of CPE with nucleic acid concentrations down to 1 nM, in plasma samples, utilizing a smart phone with an algorithm that can perform analytical testing and data processing. The detection strategy employed relies on conformational transitions between single stranded nucleic acid-cationic CPE duplexes and double stranded nucleic acid-CPE triplexes that yield distinct colorimetric responses for enabling naked eye detection of nucleic acids. Cationic poly[N,N,N-triethyl-3-((4-methylthiophen-3-yl)oxy)propan-1-aminium bromide] is utilized as the CPE reporter deposited on a polyvinylidene fluoride (PVDF) membrane for nucleic acid assay. A smart phone application is developed to capture and digitize the colorimetric response of the individual pixels of the digital images of CPE on the PVDF membrane, followed by an analysis using the algorithm. The proposed pixelated approach enables precise quantification of nucleic acid assay concentrations, thereby eliminating the margin of error involved in conventional methodologies adopted for interpretation of colorimetric responses, for instance, RGB analysis. The obtained results illustrate that a ubiquitous smart phone could be utilized for point of care colorimetric nucleic acids assays in complex matrices without requiring sophisticated software or instrumentation.

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“…Previous recent reviews published on this topic covered fundamental sensing principles, case studies and challenges faced when aiming to develop portable point‐of‐care devices for on‐site monitoring . In 2015, Monzó et al.…”

Section: Introductionmentioning

confidence: 99%

“…An overview of clinical applications of sensors for the detection of physiologically important analytes, including bacteria, was published by Justino et al. in 2016 . By comparing the analytical performance of the sensors, the authors concluded that microfluidic and lab‐on‐a‐chip devices reduce the consumption of costly reagents and shorten the experimental analysis time for the detection of bacteria significantly.…”

Section: Introductionmentioning

confidence: 99%

“…In this context, the presented review aims to provide an updated summary of biosensing strategies for the detection of pathogenic bacteria. Specific focus is laid on techniques already highlighted as most promising in previous reviews . In addition to an update on these technologies, general advances and challenges in the development of biosensors are covered.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

Kuss

Amin

Compton

2018

Chemistry An Asian Journal

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Bacterial infections represent one of the leading causes of mortality worldwide, nevertheless the design and development of rapid, cost-efficient and reliable detection methods for pathogens remains challenging. In recent years, electrochemical sensing methods have gained increasing attention for the detection of pathogenic bacteria, due to their increasingly competitive sensitivity. However, combining sensitivity with cost efficiency, high selectivity and a facile working procedure in a portable device is difficult. The presented review provides a summary of biosensing strategies for bacteria, published since 2015, by covering significant achievements towards custom-designed portable point-of-care devices. Herein, the direct chemical recognition of bacteria via enzyme activity or secretion products, as well as their detection at various electrode surfaces and materials, such as nanomaterials, indium tin oxide or paper-based immunosensors, is discussed. Furthermore, newly established hyphenated sensing principles, incorporated into lab-on-a-chip and microfluidic devices, are presented and remaining technical challenges and limitations are considered.

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Critical overview on the application of sensors and biosensors for clinical analysis

Cited by 117 publications

References 156 publications

Recent Advances in Biosensing for Neurotransmitters and Disease Biomarkers using Microelectrodes

Recent Advances in Biosensing for Neurotransmitters and Disease Biomarkers using Microelectrodes

Pixelated colorimetric nucleic acid assay

Electrochemical Detection of Pathogenic Bacteria—Recent Strategies, Advances and Challenges

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