Critical issues in application of molecular methods to environmental virology

Hamza, Ibrahim Ahmed; Bibby, Kyle

doi:10.1016/j.jviromet.2019.01.008

Cited by 41 publications

(35 citation statements)

References 190 publications

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“…Table 2 shows primers and probes for detecting and quantifying crAssphage via qPCR assay. Although molecular methods have high sensitivity and specificity, they cannot distinguish between infectious and nonviable viruses during the detection process (Hamza and Bibby, 2019). Thus, there is a need to improve the molecular technology or to find a standardized culture method to better evaluate crAssphage as a virus indicator.…”

Section: Monitoring Viruses In Water and Wastewatermentioning

confidence: 99%

Bacteriophages in water pollution control: Advantages and limitations

Liu

Sun

et al. 2020

Front. Environ. Sci. Eng.

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Section: Monitoring Viruses In Water and Wastewatermentioning

confidence: 99%

Bacteriophages in water pollution control: Advantages and limitations

Liu

Sun

et al. 2020

Front. Environ. Sci. Eng.

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“…Next-generation sequencing (NGS) facilitates to study of virus diversity using amplicon sequencing [139] . Data achieved from NGS has the potential to increase knowledge of the viral community and its diversity in the environment [140] . Apart from whole virus detection, genome, and capsid integrity of the viruses can also be detected using molecular techniques and markers [141] .…”

Section: Detection Of Virus In Environmental Matricesmentioning

confidence: 99%

SARS-CoV-2 in environmental perspective: Occurrence, persistence, surveillance, inactivation and challenges

Mohan

Hemalatha

Kopperi

et al. 2021

Chemical Engineering Journal

122

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“…Research and development are the principle tenets by this declaration, which includes understanding the prevalence of these viruses in people and the environment. These activities are crucial because risk of viral disease transmission can be interrupted through active surveillance [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Independently, laboratories have developed assays which integrate cell culture and PCR (ICC/PCR) to detect enteroviruses [22,23]. Cell culture propagates infectious virus distinguishing inactivated nucleic acid from replicating viruses, while PCR gives greater sensitivity than culture [15,[24][25][26]. This method remains inaccessible to most low resource areas due to the financial cost and access to specialized materials.…”

Section: Introductionmentioning

confidence: 99%

Optimizing a Suspension Culture Method with a Decreased Cost to Detect Enteroviruses in Water to Increase Surveillance Access

Tornberg-Belanger

Sreter

Margolin

2020

Microbiology Research

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Enteroviruses are a public health threat due to the high incidence of infections and potential for serious illness or death. Some laboratories in high-income countries detect enteroviruses in water by integrating cell culture and PCR (ICC/PCR). This combined method carries a high financial burden, due in part to specialized cell culture equipment. Therefore, we expanded upon a pilot study to reduce the cost by using common laboratory polypropylene tubes to create a cell culture in suspension. We optimized the protocol by determining minimal incubation periods post-infection as a function of the initial virus concentration. Cells in suspension and traditional monolayers were inoculated with poliovirus and incubated in 8-hour intervals up to 48 hours prior to extraction. Quantitative PCR (qPCR) was used to detect viral nucleic acid targets. Treated and raw water samples were seeded with virus and the suspension ICC/qPCR protocol used to ascertain whether the protocol performed similar to directly seeding cells. No variation in virus detection occurred using the suspension ICC/qPCR or monolayer ICC/qPCR (p = 0.95). In surface water samples, viral nucleic acid was successfully detected, with no significant increase after 32 h (p > 0.05). Suspension ICC/qPCR is as effective as monolayer ICC/qPCR in detecting enteroviruses in surface waters. Materials used in the suspension ICC/qPCR have a lower monetary cost than traditional cell culture materials without loss of sensitivity. More accessible testing of waters for enterovirus contamination through cost reduction has the potential to reduce human exposure and disease.

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Critical issues in application of molecular methods to environmental virology

Cited by 41 publications

References 190 publications

Bacteriophages in water pollution control: Advantages and limitations

Bacteriophages in water pollution control: Advantages and limitations

SARS-CoV-2 in environmental perspective: Occurrence, persistence, surveillance, inactivation and challenges

Optimizing a Suspension Culture Method with a Decreased Cost to Detect Enteroviruses in Water to Increase Surveillance Access

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