Critical evaluation of assays for vitamin D status

Lorencio, Fernando Granado; Blanco–Navarro, I.; Pérez-Sacrsitán, Belén

doi:10.1097/mco.0b013e328364ca96

Cited by 20 publications

(10 citation statements)

References 27 publications

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“…Vitamin D External Quality Assurance Scheme (DEQAS) conducts regular surveys in order to keep a track of diff erent methods used worldwide (Fraser & Milan, 2013). Automated and validated immunoassays, at present, appear to be the preferred assays for routine assessment of vitamin D nutritional status in the general population because of being cost eff ective and the ease with which these can be performed (Granado Lorencio et al 2013). However, their concordance with HPLC and LC -MS/MS could be variable (Granado Lorencio et al 2013).…”

Section: Discussionmentioning

confidence: 99%

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Urban and rural comparison of vitamin D status in Pakistani pregnant women and neonates

Anwar

Iqbal

Azam

et al. 2015

Journal of Obstetrics and Gynaecology

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We undertook a cross-sectional study in rural Jehlum and urban Karachi to evaluate the prevalence of vitamin D deficiency in Pakistani pregnant women and neonates and to assess any association of serum 25(OH) vitamin D [25(OH)D] concentration with vitamin D binding protein (Gc) genotypes. Altogether, 390 women and 266 neonates were recruited from urban and rural sites, respectively. Serum 25(OH)D was measured by an immunoassay, while Gc genotypes were identified using polymerase chain reaction followed by restriction fragment length polymorphism or PCR-RFLP. One-way analysis of variance or ANOVA and linear regression were used for statistical analysis. In urban Karachi, 99.5% of women and 97.3% of neonates were vitamin D deficient (< 50 nmol/L), while 89% of women and 82% of neonates were deficient in rural Jehlum. Gc genotypes were not associated with serum 25(OH)D concentrations in both women and their neonates. We conclude that vitamin D deficiency is highly prevalent in Pakistani women and their neonates, and Gc genotypes are not associated with serum 25(OH)D concentrations.

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Section: Discussionmentioning

confidence: 99%

“…Currently, manual immunoassays, chemiluminiscent immunoassays, HPLC, LC -MS, LC -MS/MS are deployed for vitamin D detection (Granado Lorencio et al 2013). Accuracy and specifi city have been the issues regarding 25(OH)D measurements by various methods.…”

Section: Discussionmentioning

confidence: 99%

Urban and rural comparison of vitamin D status in Pakistani pregnant women and neonates

Anwar

Iqbal

Azam

et al. 2015

Journal of Obstetrics and Gynaecology

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“…There has been lots of development work in the field, with numerous automated assays being withdrawn from the market because of poor performance, and being replaced by newly improved ones. The field has now developed to such a state that recent authoritative reviews conclude that automated vitamin D assays, if validated (traceable) can be used for the diagnosis of vitamin D nutritional status in the general population at the clinically significant concentrations of 20-375 nmol/l (67,68). Some of them even offer a sensitivity of 3-4 nmol/l, which should be sufficient to detect vitamin D deficiency.…”

Section: Oestradiolmentioning

confidence: 99%

“…Moreover, recent epidemiologic studies in men and women have demonstrated associations between low sex hormone concentrations (including E 2 ) and the risk of cardiovascular disease in both sexes (62,63,64,65), suggesting another indication where more reliable methods for E 2 measurement would be needed. (67). There has been lots of development work in the field, with numerous automated assays being withdrawn from the market because of poor performance, and being replaced by newly improved ones.…”

Section: Oestradiolmentioning

confidence: 99%

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Taylor¹,

Keevil²,

Huhtaniemi³

2015

European Journal of Endocrinology

254

157

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SummaryThe recent onslaught of mass spectrometry (MS) to measurements of steroid hormones, including demands that they should be the only acceptable method, has confused clinicians and scientists who have relied for more than 40 years on a variety of immunoassay (IA) methods in steroid hormone measurements. There is little doubt that MS methods with their superior specificity will be the future method of choice in many clinical and research applications of steroid hormone measurement. However, the majority of steroid measurements are currently, and will continue to be, carried out using various types of IAs for several reasons, including their technical ease, cost and availability of commercial reagents. Speedy replacement of all IAs with MS is an unrealistic and unnecessary goal, because the availability of MS measurements is limited by cost, need of expensive equipment, technical demands and lack of commercial applications. Furthermore, IAs have multiple well-known advantages that vindicate their continuing use. The purpose of this article is to elucidate the advantages and limitations of the MS and IA techniques from two angles, i.e. promotion of MS and defence of IA. The purpose of the text is to give the reader an unbiased view about the current state and future trends of steroid analysis and to help him/her choose the correct assay method to serve his/her diagnostic and research needs.

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“…Common assays, particularly ELISA and radio-immunoassays (RIA), are often restricted to 25(OH)D 3 because of sensitivity and specificity limitations [10][11][12]. Fortunately, recent mass spectrometry advances have permitted reaching deeper into the metabolic cascade of vitamin D, including low abundant species [13][14][15][16][17]. Consequently, a wide variety of naturally occurring metabolites of potential biological activity can be analyzed simultaneously by liquid chromatographytandem mass spectrometry (LC-MS/MS), which is currently considered the "gold standard" technique.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Low Abundant Vitamin D Metabolites and Assaying Their Distribution in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) as a New Tool for Diagnosis and Risk Prediction of Vitamin DRelated Diseases

Kattner¹,

Volmer²

2017

A Critical Evaluation of Vitamin D - Basic Overview

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This chapter provides an overview of versatile and efficient chemical syntheses of vitamin D derivatives by application of either linear or convergent synthesis approaches. Synthesis of the most relevant naturally occurring vitamin D metabolites and their deuterated counterparts to use as calibration and reference standards in LC-MS/MS assays is also shown. The chapter then summarizes the most important mass spectrometric approaches to quantify important vitamin D metabolites in human biofluids. In addition, new developments are described that are aimed at the pathobiological interpretation of the measured vitamin D metabolite distributions in various human diseases.

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Critical evaluation of assays for vitamin D status

Cited by 20 publications

References 27 publications

Urban and rural comparison of vitamin D status in Pakistani pregnant women and neonates

Urban and rural comparison of vitamin D status in Pakistani pregnant women and neonates

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Synthesis of Low Abundant Vitamin D Metabolites and Assaying Their Distribution in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) as a New Tool for Diagnosis and Risk Prediction of Vitamin DRelated Diseases

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