Critical evaluation of aptamer binding for biosensor designs

Comparing two cortisol aptamers for label-free fluorescent and colorimetric biosensors

“…Recently, a number of papers have been published to scrutinize the binding of some commonly used aptamers 52 for important analytes such as arsenic, 53 ethanolamine, 54 ampicillin, 55 and some pesticides. 56 None of them showed binding.…”

Homogeneous assays for aptamer-based ethanolamine sensing: no indication of target binding

“…In our study using three different homogeneous binding assays, all the tested sequences behaved similarly regardless of whether they were previously reported to bind ethanolamine or not. This study indicates the importance of using control sequences and multiple different assays to confirm aptamer binding, 36,37 and especially the importance of using homogeneous assays for studying free target molecules.…”

“…Recently, quite a few papers have been published to scrutinize the aptamers for a few small molecules such as arsenic, ampicillin, chloramphenicol and a few pesticides, [30][31][32][33][34][35] where careful homogeneous binding assays revealed no binding. 36 Given that heterogeneous aptamer binding assays are more difficult to interpret, 37,38 and intrigued by the reported binding assays for ethanolamine, in this work, we examined the binding of the ethanolamine aptamers using homogeneous binding assays.…”

Novel electrochemiluminescent assay for the aptamer-based detection of testosterone

“…Besides, aptamers also show few disadvantages such as: i) susceptibility to nuclease degradation, ii) limited building block diversity, iii) PCR bias in SELEX method and iv) long aptameric sequences may interfere with the reproducibility and accuracy of the biosensors [1]. Another aspect that is important to mention is that the number of non-binding aptamers has been increasing showing that a multi-technique characterization is needed [8][9][10].…”