Critical analysis and systematization of rat pancreatectomy terminology

Eulálio, José Marcus Raso; Bon-Habib, Assad Charbel Chequer; Soares, Daiane de Oliveira; Correa, Paulo; Pineschi, Giovana Penna Firme; Diniz, Victor; Manso, José Eduardo Ferreira; Schanaider, Alberto

doi:10.1590/s0102-865020160100000010

Cited by 8 publications

(6 citation statements)

References 20 publications

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“…The percentage of animals induced into diabetic status after an intravenous streptozotocin injection is higher than with the use of alloxan. In this study, there was a 100% success rate in inducing diabetes 24 hours after the intravenous STZ injection [9][10][11] .…”

Section: ■ Discussionmentioning

confidence: 63%

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A non-inferiority study to compare daily fast-acting insulin versus twice a week slow-acting insulin–moderate diabetes mode

Camargo

Weschenfelder²,

Fonseca³

et al. 2020

Acta Cir. Bras.

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Section: ■ Discussionmentioning

confidence: 63%

“…One possibility are genetically modified animals (WDF / TA-FA, Zucker) that can be adopted as models of diabetes-associated with metabolic syndrome. 9 Although the model is valid, there are high costs and reproducibility is difficult.…”

Section: ■ Discussionmentioning

confidence: 99%

A non-inferiority study to compare daily fast-acting insulin versus twice a week slow-acting insulin–moderate diabetes mode

Camargo

Weschenfelder²,

Fonseca³

et al. 2020

Acta Cir. Bras.

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“…The laparoscopy causes less trauma 13 , postoperative pain 14 , dehydration due to exposure of loops 15 , and often with fewer postoperative complications. The distal pancreatectomy with splenectomy technique in rats was not found in the literature 16 .…”

Section: ■ Discussionmentioning

confidence: 99%

A technical note on low cost rat laparoscopy an initial experience

et al. 2018

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Purpose: To evaluate a novel and adapted low-cost set model for laparoscopic surgery in rats. Methods: Nine Wistar rats underwent two different laparoscopic procedures, splenectomy (n=3) and distal pancreatectomy with splenectomy (n = 6), after assembling a low-cost set replacing the conventional one (monitor, micro camera, image processor, light source, laparoscope and insufflator). The new set included an Android Tablet 10.5 '', a 5mm USB Endoscope and semiautomatic sphygmomanometer monitor. Results: The same surgeon performed the laparoscopic procedures. Total surgical time ranged from 36 to 60 minutes with a mean of 45.8 minutes. Three rats died during the distal pancreatic and splenectomy procedure (33.3%), due to respiratory failure (n = 1), uncontrolled abdominal hemorrhage (n=1) and iatrogenic gastric perforation (n = 1). We followed the other six rats (66.6%) for seven days with no further evidence of complications. Conclusions: The laparoscopic partial pancreatectomy and splenectomy can be performed with the novel low-cost set assembled in the present experimental study. Both specific training and skills development are required to validate more advanced laparoscopic procedures and achieve a desirable outcome.

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“…The use of STZ in this context is however suboptimal, since the toxin also has the potential to induce direct nephrotoxic and cardiotoxic effects, independent of hyperglycemia 24 – 26 . Pancreatectomy (Px), on the other hand, is a well-characterized method of surgically inducing diabetes in rats 27 . An important advantage of this model is that it reflects the isolated effects of a reduced beta cell mass; immediately after surgery Px rats become insulin deficient and hyperglycemic, allowing full control of diabetic onset 28 .…”

Section: Introductionmentioning

confidence: 99%

Rat pancreatectomy combined with isoprenaline or uninephrectomy as models of diabetic cardiomyopathy or nephropathy

Thisted

Østergaard

Pedersen

et al. 2020

Sci Rep

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Cardiovascular and renal complications are the predominant causes of morbidity and mortality amongst patients with diabetes. Development of novel treatments have been hampered by the lack of available animal models recapitulating the human disease. We hypothesized that experimental diabetes in rats combined with a cardiac or renal stressor, would mimic diabetic cardiomyopathy and nephropathy, respectively. Diabetes was surgically induced in male Sprague Dawley rats by 90% pancreatectomy (Px). Isoprenaline (Iso, 1 mg/kg, sc., 10 days) was administered 5 weeks after Px with the aim of inducing cardiomyopathy, and cardiac function and remodeling was assessed by echocardiography 10 weeks after surgery. Left ventricular (LV) fibrosis was quantified by Picro Sirius Red and gene expression analysis. Nephropathy was induced by Px combined with uninephrectomy (Px-UNx). Kidney function was assessed by measurement of glomerular filtration rate (GFR) and urine albumin excretion, and kidney injury was evaluated by histopathology and gene expression analysis. Px resulted in stable hyperglycemia, hypoinsulinemia, decreased C-peptide, and increased glycated hemoglobin (HbA1c) compared with sham-operated controls. Moreover, Px increased heart and LV weights and dimensions and caused a shift from α-myosin heavy chain (MHC) to β-MHC gene expression. Isoprenaline treatment, but not Px, decreased ejection fraction and induced LV fibrosis. There was no apparent interaction between Px and Iso treatment. The superimposition of Px and UNx increased GFR, indicating hyperfiltration. Compared with sham-operated controls, Px-UNx induced albuminuria and increased urine markers of kidney injury, including neutrophil gelatinase-associated lipocalin (NGAL) and podocalyxin, concomitant with upregulated renal gene expression of NGAL and kidney injury molecule 1 (KIM-1). Whereas Px and isoprenaline separately produced clinical endpoints related to diabetic cardiomyopathy, the combination of the two did not accentuate disease development. Conversely, Px in combination with UNx resulted in several clinical hallmarks of diabetic nephropathy indicative of early disease development.

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Critical analysis and systematization of rat pancreatectomy terminology

Cited by 8 publications

References 20 publications

A non-inferiority study to compare daily fast-acting insulin versus twice a week slow-acting insulin–moderate diabetes mode

A non-inferiority study to compare daily fast-acting insulin versus twice a week slow-acting insulin–moderate diabetes mode

A technical note on low cost rat laparoscopy an initial experience

Rat pancreatectomy combined with isoprenaline or uninephrectomy as models of diabetic cardiomyopathy or nephropathy

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