CRISPR/Cas9-Mediated Knockout Reveals the Involvement of CYP304F1 in β-Cypermethrin and Chlorpyrifos Resistance in <i>Spodoptera litura</i>

Xu, Li; Li, Bo; Liu, Hongyu; Zhang, Hongwei; Liu, Runqiang; Yu, Hao; Li, Dong-Zhi

doi:10.1021/acs.jafc.2c04352

Cited by 9 publications

(6 citation statements)

References 45 publications

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“…The overexpression of CYP321B1 plays roles in β‐cypermethrin and cyhalothrin resistance validated by RNAi (Xu et al, 2020). The relative expression of SlGSTe8 was found to be significantly higher in the QJ population than the GX population by RNA‐Seq in our previous study (Xu et al, 2022) and validated by qRT‐PCR in this study. S. litura has relatively large amounts of GSTs genes among most insect species (Hu et al, 2022).…”

Section: Discussionsupporting

confidence: 80%

“…In Bombyx mori, BmGSTO was induced by diazinon, permethrin, imidacloprid, E. coli and UV-B and might be involved in protecting cells against oxidative stress (Yamamoto , 2011). In Nilaparvata lugens, a notorious insect with serious insecticide resistance, NlGSTd2 was reported to confer resistance to pyrethroid insecticide as an antioxidant agent (Vontas et al, 2001(Vontas et al, , 2002; NlGSTs1 and NlGSTs2 conferred resistance to chlorpyrifos, fipronil, etofenprox, imidacloprid and even the secondary metabolites from host plant through antioxidation function (Zhang et al, 2022). In this study, the recombinant protein SlGSTE8…”

Section: Discussionmentioning

confidence: 74%

“…In the RNA-Seq results of our previous study (Xu et al, 2022), SlGSTe8 showed significantly higher reads in a field-collected population resistant to pyrethroids and organophosphates than the susceptible population (Table S1, NCBI Sequence Read Archive database with accession number PRJNA843172). In this study, the contribution of SlGSTE8 in the resistance to pyrethroids and organophosphates was explored by heterologous expression and disc diffusion assay.…”

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confidence: 97%

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SlGSTE8 in Spodoptera litura participated in the resistance to phoxim and chlorpyrifos

Liu

et al. 2023

J Applied Entomology

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Glutathione S‐transferases (GSTs) were reported to participate in insecticide resistance by metabolic and antioxidant activities. In our previous study, an ε class gene of GSTs, SlGSTe8 in Spodoptera litura, was screened out to be upregulated in a population resistant to pyrethroids and organophosphates. SlGSTe8 was highly expressed in the larvae stage, and the digestive tissue, foregut, midgut and hindgut, while the relative expression level was low in the pupae stage and other tissues. To further explore its role in the resistance to pyrethroids and organophosphates, the metabolic activity to insecticides by its recombinant protein was determined by Ultra Performance Liquid Chromatography, and its antioxidant enzyme activity was evaluated by disc diffusion assay. The recombinant protein showed significant metabolic activity to phoxim and chlorpyrifos, but not to fenvalerate, cyhalothrin or β‐cypermethrin. After incubation, the depletion rate of chlorpyrifos is 85.3%, higher than that of phoxim (17.5%). Also, the inhibition zone around filter discs decreased significantly after exposure to cumene hydroperoxide in recombinant plasmid than vector only, suggesting significant antioxidant activity of SlGSTE8. Further modelling and docking analysis indicated that the 3D structure of SlGSTE8 was well shaped for phoxim and chlorpyrifos, with the binding energy −5.58 and −5.15 kcal/mol, respectively. Our work provides evidence that SlGSTe8 in S. litura plays important roles in phoxim and chlorpyrifos resistance.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 74%

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confidence: 97%

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SlGSTE8 in Spodoptera litura participated in the resistance to phoxim and chlorpyrifos

Liu

et al. 2023

J Applied Entomology

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“…This outcome is particularly encouraging as S. litura is among the species of insects which has developed resistance towards CPF. [18] There are several reports in literature regarding the development of bio-based safe pesticides but their synthesis is not convenient (multistep, drastic reaction conditions). [19,20] In contrast, PBI-SAH building block is easy to prepare and is highly fluorescent in aqueous media.…”

Section: Introductionmentioning

confidence: 99%

“…The present investigation also describes the high efficiency of PBI‐SAH assemblies to disturb the life cycle of Spodoptera litura ( S. litura ) and to exhibit 100 % insecticidal activity after 19 days. This outcome is particularly encouraging as S. litura is among the species of insects which has developed resistance towards CPF [18] . There are several reports in literature regarding the development of bio‐based safe pesticides but their synthesis is not convenient (multistep, drastic reaction conditions) [19,20] .…”

Section: Introductionmentioning

confidence: 99%

Beyond Molecular Recognition: A Perylene Bisimide Derivative as a Functional Mimic of Chlorpyrifos

Devi,

Sharma,

Sharma

et al. 2023

Chemistry An Asian Journal

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Supramolecular assemblies of perylene bisimide derivative (PBI‐SAH) have been developed which show ‘turn‐on’ detection of chlorpyrifos in aqueous media, apple residue and blood serum. Differently from the already reported fluorescent probes for the detection of CPF, PBI‐SAH assemblies also show affinity for acetylcholinesterase (AChE) which endow the PBI‐SAH molecules with mixed inhibitory potential to restrict the AChE catalysed hydrolysis of acetylthiocholine (ATCh) in MG‐63 cell lines (in vitro) and in mice (in vivo). The molecular docking studies support the inhibitory activity of PBI‐SAH assemblies and their potential to act as safe insecticide with high benefit to harm ratio. The insecticidal potential of PBI‐SAH derivative has been examined against Spodoptera litura (S. litura) and these studies demonstrate its excellent insecticidal activity (100 % mortality in nineteen days). To the best of our knowledge, this is the first report regarding development of PBI‐SAH assemblies which not only detect chlorpyrifos but also mimic AChE inhibitory activity of CPF to show promising aptitude as safe insecticide.

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Expression and functional analysis of ace1 and ace2 reveal their differential roles in larval growth and insecticide sensitivity in Spodoptera frugiperda (J. E. Smith, 1797)

et al. 2023

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Acetylcholinesterase (AChE, EC3.1.1.7) is a key enzyme in neuronal signal transduction that hydrolyzes the neurotransmitter acetylcholine (ACh). The toxicological and physiological functions of AChEs in the destructive pest Spodoptera frugiperda, however, are still exclusive. In the present study, two acetylcholinesterase genes, ace1 and ace2, were characterized from S. frugiperda transcriptome and genome. Spatial−temporal expression analysis indicated that both Sfruace1 and Sfruace2 had an enriched expression in the heads among all larval tissues, and in 3 rd instar larvae among all developmental stages. Notably, the expression level of Sfruace1 is much higher than Sfruace2 in all tested tissues and developmental stages. The RNA interference (RNAi) with speci c designed small interfering RNA (siRNA) signi cantly reduced the expression of Sfruace1 to 30%, and Sfruace2 to 39%. The knockdown of Sfruace1 expression resulted in mortality of 37.6%, which is signi cantly higher than 17.06% in the siSfruace2-treated group. Furthermore, the RNAi of Sfruace1 and Sfruace2 expressions reduced the AChE enzymatic activity to 32.81% and 65.77%, respectively, compared with those of the untreated group, 24 h after injection of 70 ng siRNA per insect. The survivors after the siRNA treatments showed an apparent motor retardation to the arti cial diet, and an apparent growth inhibition in F 0 generation larvae. There was no signi cant inhibition effect on the growth of F 0 and F 1 generation pupae and the fecundity of female adult. Insecticide bioassay showed that the siSfruace1-treated larvae were more susceptible to acephate than the siSfruace2-treated and untreated larvae. Our study suggests that Sfruace1 plays a more important role in larval survivorship and susceptibility to acephate than Sfruace2 did in S. frugiperda, and both ace genes may have differential roles in regulating larvae growth, motor ability and insecticidal sensitivity in S. frugiperda.

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CRISPR/Cas9-Mediated Knockout Reveals the Involvement of CYP304F1 in β-Cypermethrin and Chlorpyrifos Resistance in Spodoptera litura

Cited by 9 publications

References 45 publications

SlGSTE8 in Spodoptera litura participated in the resistance to phoxim and chlorpyrifos

SlGSTE8 in Spodoptera litura participated in the resistance to phoxim and chlorpyrifos

Beyond Molecular Recognition: A Perylene Bisimide Derivative as a Functional Mimic of Chlorpyrifos

Expression and functional analysis of ace1 and ace2 reveal their differential roles in larval growth and insecticide sensitivity in Spodoptera frugiperda (J. E. Smith, 1797)

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