CRISPR/Cas9 mediated disruption of Inositol Pentakisphosphate 2-Kinase 1 (TaIPK1) reduces phytic acid and improves iron and zinc accumulation in wheat grains

Ibrahim, Saira; Saleem, Bilal; Rehman, Nazia; Zafar, Syed Adeel; Naeem, Muhammad Kashif; Khan, Muhammad Ramzan

doi:10.1016/j.jare.2021.07.006

Cited by 72 publications

(24 citation statements)

References 35 publications

(43 reference statements)

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“…Although genetic improvement in potato has limitations due to its genome complexity, it still presents a good opportunity to engineer useful traits. CRISPR/Cas9 is a widely adopted and sophisticated tool for crop genome engineering that specifically targets the desired gene(s) ( Zafar et al, 2020a ; Ibrahim et al, 2021 ). It can produce insertions, deletions, substitutions, or point mutations (SNPs) at a specified location in the target gene.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E-Enhanced Resistance Against Potato Virus Y

et al. 2022

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Potato (Solanum tuberosum L.) is an important staple food around the world, and potato virus Y (PVY) is a major constraint for potato production. The VPg protein of PVY interacts with the translation initiation factor eIF4E of the host that works as a susceptibility factor during infection. The interaction between eIF4E and VPg was disrupted by CRISPR/Cas9. The homozygous conserved region of eIF4E of the potato variety “Kruda” was mutated by CRISPR/Cas9. Tracking of insertion, deletion, and conversion events was performed by Sanger sequencing with ∼15% editing efficiency. Truncated and mutated eIF4E proteins were unable to interact with VPg, and the virus was not able to exploit the host machinery for replication and systemic spreading. Mutated eIF4E lines showed enhanced resistance to PVYO strain. DAS-ELISA and RT-PCR were used for validation of the observed resistance. PVY resistance in tetraploid lines via CRISPR/Cas9 provides a route to develop novel resistant potato cultivars.

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Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E-Enhanced Resistance Against Potato Virus Y

et al. 2022

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“…A 35% decrease in phytic acid and a simultaneous increase in P i were found in the mutants [90]. Using CRISPR/Cas9 in common wheat, the disruption of TaIPK1.A led to an approxi-mately 1.5-to 2.1-fold increase in the Fe concentration and to a 1.6-to-1.9-fold rise in Zn concentration due to significant reduction in phytic acid content [78].…”

Section: Quality Improvementmentioning

confidence: 98%

CRISPR towards a Sustainable Agriculture

2022

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Climate change and the need to feed an increasing population undermines food production and safety, representing the reasons behind the development of a new agriculture that is much more sustainable, productive and accessible worldwide. Genome editing and, in particular, clustered regularly interspaced palindromic repeats/CRISPR-associated protein (CRISPR/Cas) tools will play a major role in plant breeding to address these concerns. CRISPR/Cas includes a series of genome editing tools relying on the recognition and cleavage of target DNA/RNA sequences to introduce specific mutations.

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“…CRISPR-Cas9 is responsible for the latter type of biofortification. This system has been reported to disrupt the Inositol pentakisphosphate 2- kinase 1 ( IPK1 ) gene causing iron biofortification in wheat ( Ibrahim et al, 2021 ).…”

Section: Development Of Biofortified Crops Through Crispr-cas Genome ...mentioning

confidence: 99%

“…There is only one report on Zn enhancement in wheat, to the best of our knowledge. In this crop, the CRISPR-Cas system disrupts Triticum aestivum Inositol Pentakisphosphate 2-kinase 1 (TaIPK1) that reduces phytic acid to cause improvement in zinc accumulation in wheat grains ( Ibrahim et al, 2021 ). More crops are needed to be biofortified with Zn through the CRISPR-Cas system to make available the products worldwide.…”

Section: Development Of Biofortified Crops Through Crispr-cas Genome ...mentioning

confidence: 99%

CRISPR-Based Genome Editing for Nutrient Enrichment in Crops: A Promising Approach Toward Global Food Security

et al. 2022

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The global malnutrition burden imparts long-term developmental, economic, social, and medical consequences to individuals, communities, and countries. The current developments in biotechnology have infused biofortification in several food crops to fight malnutrition. However, these methods are not sustainable and suffer from several limitations, which are being solved by the CRISPR-Cas-based system of genome editing. The pin-pointed approach of CRISPR-based genome editing has made it a top-notch method due to targeted gene editing, thus making it free from ethical issues faced by transgenic crops. The CRISPR-Cas genome-editing tool has been extensively used in crop improvement programs due to its more straightforward design, low methodology cost, high efficiency, good reproducibility, and quick cycle. The system is now being utilized in the biofortification of cereal crops such as rice, wheat, barley, and maize, including vegetable crops such as potato and tomato. The CRISPR-Cas-based crop genome editing has been utilized in imparting/producing qualitative enhancement in aroma, shelf life, sweetness, and quantitative improvement in starch, protein, gamma-aminobutyric acid (GABA), oleic acid, anthocyanin, phytic acid, gluten, and steroidal glycoalkaloid contents. Some varieties have even been modified to become disease and stress-resistant. Thus, the present review critically discusses CRISPR-Cas genome editing-based biofortification of crops for imparting nutraceutical properties.

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CRISPR/Cas9 mediated disruption of Inositol Pentakisphosphate 2-Kinase 1 (TaIPK1) reduces phytic acid and improves iron and zinc accumulation in wheat grains

Cited by 72 publications

References 35 publications

CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E-Enhanced Resistance Against Potato Virus Y

CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E-Enhanced Resistance Against Potato Virus Y

CRISPR towards a Sustainable Agriculture

CRISPR-Based Genome Editing for Nutrient Enrichment in Crops: A Promising Approach Toward Global Food Security

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