CRISPR/Cas9: A Tool to Circumscribe Cotton Leaf Curl Disease

Iqbal, Zafar; Sattar, Muhammad Naeem; Shafiq, Muhammad

doi:10.3389/fpls.2016.00475

Cited by 96 publications

(45 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cotton leaf curl disease is a major biotic constraint for cotton production in Pakistan since the last two decades. After the occurrence of first epidemic in Pakistan, plant breeders are working for permanent and long lasting solution to develop resistance in high yielding varieties (Abbas et al, 2015;Iqbal et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Molecular Screening of Cotton Germplasm for Cotton Leaf Curl Disease Caused by Viral Strains

Saleem¹,

Khan²,

Azhar³

et al. 2017

IJAB

View full text Add to dashboard Cite

To cite this paper: Slaeem, H., A.A. Khan, M.T. Azhar and M.S. Nawaz-ul-Rehman, 2017. Molecular screening of cotton germplasm for cotton leaf curl disease caused by viral strains. Int. J. Agric. AbstractCotton crop is infected by single stranded DNA viruses (Family, Geminiviridae) in Pakistan. Due to changing global climate, which favors the whitefly (insect vector) growth and reproduction, the spread of cotton leaf curl disease is increasing at an alarming rate. Various approaches are being used to diagnose the ssDNA viruses in cotton. One step PCR protocol was established to identify the three viruses, namely cotton leaf curl Multan virus (CLCuMuV), Burewala strain of Kokhran virus (CLCuKo-Bur) and cotton leaf curl Kokhran virus (CLCuKoV). Thirty eight genotypes were screened on the basis of symptoms and molecular diagnosis. Out of 38 genotypes, 13 were positive for CLCuMuV, 24 were positive for Burewala strain and one was positive for both CLCuMuV and CLCuKoV-Bur strain. However, none of the samples was positives for cotton leaf curl Kokhran virus. Our experiments have established one step guide PCR for screening of cotton germplasm for major cotton infecting viruses. This diagnostic assay will promote the breeding programs for the development of resistant cotton varieties against cotton leaf curl disease (CLCuD) in Pakistan.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Screening of Cotton Germplasm for Cotton Leaf Curl Disease Caused by Viral Strains

Saleem¹,

Khan²,

Azhar³

et al. 2017

IJAB

View full text Add to dashboard Cite

show abstract

“…42% of the mutant plants harbored desired mutations and 23.5% of the mutants showed resistance to citrus canker. The stacking up of multiple nucleases as one transgene by CRISPR/Cas9 system also leads to the targeted cleavage of multiple infections by viruses ( Iqbal et al, 2016 ).…”

Section: Targeted Genome Modification In Crop Plantsmentioning

confidence: 99%

Gene Editing and Crop Improvement Using CRISPR-Cas9 System

2017

View full text Add to dashboard Cite

Advancements in Genome editing technologies have revolutionized the fields of functional genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-Cas9 is a multipurpose technology for genetic engineering that relies on the complementarity of the guideRNA (gRNA) to a specific sequence and the Cas9 endonuclease activity. It has broadened the agricultural research area, bringing in new opportunities to develop novel plant varieties with deletion of detrimental traits or addition of significant characters. This RNA guided genome editing technology is turning out to be a groundbreaking innovation in distinct branches of plant biology. CRISPR technology is constantly advancing including options for various genetic manipulations like generating knockouts; making precise modifications, multiplex genome engineering, and activation and repression of target genes. The review highlights the progression throughout the CRISPR legacy. We have studied the rapid evolution of CRISPR/Cas9 tools with myriad functionalities, capabilities, and specialized applications. Among varied diligences, plant nutritional improvement, enhancement of plant disease resistance and production of drought tolerant plants are reviewed. The review also includes some information on traditional delivery methods of Cas9-gRNA complexes into plant cells and incorporates the advent of CRISPR ribonucleoproteins (RNPs) that came up as a solution to various limitations that prevailed with plasmid-based CRISPR system.

show abstract

“…Furthermore its potential in controlling begomoviruses can be explored due to robustness, wide adaptability and ease in engineering of this system (Iqbal et al, 2016). …”

Section: Genome Editing Approach: Crispr/cas Resistance Strategymentioning

confidence: 99%

Genetics and Genomics of Cotton Leaf Curl Disease, Its Viral Causal Agents and Whitefly Vector: A Way Forward to Sustain Cotton Fiber Security

et al. 2017

View full text Add to dashboard Cite

Cotton leaf curl disease (CLCuD) after its first epidemic in 1912 in Nigeria, has spread to different cotton growing countries including United States, Pakistan, India, and China. The disease is of viral origin—transmitted by the whitefly Bemisia tabaci, which is difficult to control because of the prevalence of multiple virulent viral strains or related species. The problem is further complicated as the CLCuD causing virus complex has a higher recombination rate. The availability of alternate host crops like tomato, okra, etc., and practicing mixed type farming system have further exaggerated the situation by adding synergy to the evolution of new viral strains and vectors. Efforts to control this disease using host plant resistance remained successful using two gene based-resistance that was broken by the evolution of new resistance breaking strain called Burewala virus. Development of transgenic cotton using both pathogen and non-pathogenic derived approaches are in progress. In future, screening for new forms of host resistance, use of DNA markers for the rapid incorporation of resistance into adapted cultivars overlaid with transgenics and using genome editing by CRISPR/Cas system would be instrumental in adding multiple layers of defense to control the disease—thus cotton fiber production will be sustained.

show abstract

CRISPR/Cas9: A Tool to Circumscribe Cotton Leaf Curl Disease

Cited by 96 publications

References 86 publications

Molecular Screening of Cotton Germplasm for Cotton Leaf Curl Disease Caused by Viral Strains

Molecular Screening of Cotton Germplasm for Cotton Leaf Curl Disease Caused by Viral Strains

Gene Editing and Crop Improvement Using CRISPR-Cas9 System

Genetics and Genomics of Cotton Leaf Curl Disease, Its Viral Causal Agents and Whitefly Vector: A Way Forward to Sustain Cotton Fiber Security

Contact Info

Product

Resources

About