Molecular Screening of Cotton Germplasm for Cotton Leaf Curl Disease Caused by Viral Strains

Abstract

To cite this paper: Slaeem, H., A.A. Khan, M.T. Azhar and M.S. Nawaz-ul-Rehman, 2017. Molecular screening of cotton germplasm for cotton leaf curl disease caused by viral strains. Int. J. Agric. AbstractCotton crop is infected by single stranded DNA viruses (Family, Geminiviridae) in Pakistan. Due to changing global climate, which favors the whitefly (insect vector) growth and reproduction, the spread of cotton leaf curl disease is increasing at an alarming rate. Various approaches are being used to diagnose the ssDNA viruses in cotton. One step PCR protocol was established to identify the three viruses, namely cotton leaf curl Multan virus (CLCuMuV), Burewala strain of Kokhran virus (CLCuKo-Bur) and cotton leaf curl Kokhran virus (CLCuKoV). Thirty eight genotypes were screened on the basis of symptoms and molecular diagnosis. Out of 38 genotypes, 13 were positive for CLCuMuV, 24 were positive for Burewala strain and one was positive for both CLCuMuV and CLCuKoV-Bur strain. However, none of the samples was positives for cotton leaf curl Kokhran virus. Our experiments have established one step guide PCR for screening of cotton germplasm for major cotton infecting viruses. This diagnostic assay will promote the breeding programs for the development of resistant cotton varieties against cotton leaf curl disease (CLCuD) in Pakistan.

Soil Manuring and Genetic Variation Conjunctively Surmount the Partial Drought Stress in Wheat (Triticum aestivum L.)

“…During the decomposition of organic matter, release of nutrients and organic compounds increases growth and yield traits (Hendrix et al, 1994). Similar results were reported by Iqbal et al (2010). Shah et al (2022) reported similar results regarding spike length.…”

“…Different investigators have found that turgor decreases due to water stress conditions, but membrane stability does not get affected under water stress conditions (Jatoi et al, 2011). Similar results were reported by (Iqbal et al, 2010) for various wheat cultivars under water stress levels. Relative water contents have been reported as one of the key signs of water stress in plant leaves and is closely associated with cell volume, thus it may more accurately reflects the balance between availability of water to leaves and transpiration rate (Merah, 2001).…”

Mapping of quantitative trait loci controlling cotton leaf curl disease resistance in upland cotton

“…We observed a wide range of responses among 100 elite cultivars/varieties during the initial screening against CLCuD (Javed et al, 2017). Based on germplasm screening and the available literature (Batool et al, 2021; Javed et al, 2017; Saleem et al, 2017), a highly tolerant genotype MNH‐886 and the highly susceptible genotype S‐12 were chosen to develop their segregating plant populations. The phenotypic performance for CLCuD severity of both parents (MNH‐886 and S‐12) and their segregating F2 and F3 populations were further observed for two seasons (Table 1).…”