CRISPR/Cas12a system responsive DNA hydrogel for label-free detection of non-glucose targets with a portable personal glucose meter

Ma, Wenxiao; Liu, Minghui; Xie, Shupu; Liu, Bo; Jiang, Lizhi; Zhang, Xiaoru; Yuan, Xunyi

doi:10.1016/j.aca.2022.340439

Cited by 16 publications

(10 citation statements)

References 34 publications

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“…This method allows for a rapid, sensitive and specific analysis of DNA and provides a new pathway for oligonucleic acids amplification. Recently, a large amount of research has focused on using the CRISPR-Cas12a system in amplifying biomolecular assays. − For example, Lu et al used an aptamer and DNAzyme regulated CRISPR-Cas12a sensor for fluorometric diagnosis of the non-nucleic acid target . Dai et al constructed a universal electrochemical biosensor based on CRISPR-Cas12a system .…”

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confidence: 99%

Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction

et al. 2023

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Herein, a chemiluminescence (CL) biosensor based on CRISPR-Cas12a and cation exchange reaction was constructed to detect the biomarker microRNA-21 (miRNA-21). The rolling circle amplification (RCA) reaction was introduced to convert each target RNA strand into a long single-stranded DNA with repeated sequences, which acted as triggers to initiate the transcleavage activity of CRISPR-Cas12a. The activated Cas12a could cleave the biotinylated linker DNA of CuS nanoparticles (NPs) to inhibit the binding of CuS NPs to streptavidin immobilized on the surface of the microplate, which strongly reduced the generation of Cu2+ from a cation exchange between CuS NPs and AgNO3, and thus efficiently suppressed the CL of Cu2+-luminol-H2O2 system, giving a “signal off” biosensor. With the multiple amplification, the detection limit of the developed sensor for miRNA-21 reached 16 aM. In addition, this biosensor is not only suitable for a professional chemiluminescence instrument but also for a smartphone used as a detection tool for the purpose of portable and low-cost assay. This method could be used to specifically detect quite a low level of miRNA-21 in human serum samples and various cancer cells, indicating its potential in ultrasensitive molecular diagnostics.

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confidence: 99%

Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction

et al. 2023

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“…In 2022, Ma et al amplifies the detection of SARS-CoV-2 N-gene (nucleocapsid phosphoprotein gene) and PCB77 (3,3′,4,4′-tetrachlorobiphenyl) by CRISPR-responsive DNA hydrogels combined with PGM, solving the above problems. [104] Specifically, when the target gene is present, it hybridizes to the matching sequence and then undergoes cyclization and RCA process to produce a DNA product with a long repeat that binds Cas12a/sgRNA. The Cas12a is activated to cut the ssDNA cross-linkers, gel transforming sol.…”

Section: Biosensingmentioning

confidence: 99%

Stimuli‐Responsive Self‐Degradable DNA Hydrogels: Design, Synthesis, and Applications

Wang

Duan

Liu

et al. 2023

Adv Healthcare Materials

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DNA hydrogels play an increasingly important role in biomedicine and bioanalysis applications. Due to their high programmability, multifunctionality and biocompatibility, they are often used as effective carriers for packing drugs, cells, or other bioactive cargoes in vitro and in vivo. However, the stability of the DNA hydrogels prevents their in‐demand rapid release of cargoes to achieve a full therapeutic effect in time. For bioanalysis, the generation of signals sometimes needs the DNA hydrogel to be rapidly degraded when sensing target molecules. To meet these requirements, stimulus‐responsive DNA hydrogels are designed. By responding to different stimuli, self‐degradable DNA hydrogels can switch from gel to solution for quantitative bioanalysis and precision cargo delivery. This review summarizes the recently developed innovative methods for designing stimuli‐responsive self‐degradable DNA hydrogels and showed their applications in the bioanalysis and biomedicines fields. Challenges, as well as prospects, are also discussed.

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“…For example, Cas12a nuclease, when activated by crRNA, is capable of non-specifically cleaving long-stranded DNA at specific binding sites. Ma et al [ 66 ] designed a Cas12a/crRNA enzyme-responsive DNA hydrogel for the detection of specific genes. In the presence of target gene, Cas12a enzyme activity was activated and led to the disruption of the cross-links, thereby releasing the invertase, which converted sucrose to glucose for signal readout with a glucose meter ( Figure 3 F).…”

Section: Preparation and Classification Of Stimulus-responsive Dna Hy...mentioning

confidence: 99%

“…( F ) Schematic representation of the DNA hydrogel sensing platform for sensing N-gene via a PGM. Copyright (2022) Elsevier [ 66 ].…”

Section: Figurementioning

confidence: 99%

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Wang

Lai

et al. 2023

Biosensors

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Food safety has always been a major global challenge to human health and the effective detection of harmful substances in food can reduce the risk to human health. However, the food industry has been plagued by a lack of effective and sensitive safety monitoring methods due to the tension between the cost and effectiveness of monitoring. DNA-based hydrogels combine the advantages of biocompatibility, programmability, the molecular recognition of DNA molecules, and the hydrophilicity of hydrogels, making them a hotspot in the research field of new nanomaterials. The stimulus response property greatly broadens the function and application range of DNA hydrogel. In recent years, DNA hydrogels based on stimulus-responsive mechanisms have been widely applied in the field of biosensing for the detection of a variety of target substances, including various food contaminants. In this review, we describe the recent advances in the preparation of stimuli-responsive DNA hydrogels, highlighting the progress of its application in food safety detection. Finally, we also discuss the challenges and future application of stimulus-responsive DNA hydrogels.

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CRISPR/Cas12a system responsive DNA hydrogel for label-free detection of non-glucose targets with a portable personal glucose meter

Cited by 16 publications

References 34 publications

Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction

Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction

Stimuli‐Responsive Self‐Degradable DNA Hydrogels: Design, Synthesis, and Applications

Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

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