Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Wang, Huiyuan; Wang, Xinyu; Lai, Keqiang; Yan, Juan

doi:10.3390/bios13030320

Cited by 16 publications

(8 citation statements)

References 183 publications

(218 reference statements)

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“…Our system improves on present qPCR methods for many important applications, especially considering the simplified workflow, analysis time, probe costs, and simplified detector. These may include viral pathogen detection, − environmental surveillance, − or food quality applications. − We expect that future iterations will provide quantitative data.…”

Section: Resultsmentioning

confidence: 99%

Biosensing with Polymerase Chain Reaction-Stable DNA-Functionalized Magnetically Susceptible Carbon–Iron Microparticles

Koirala,

Dalbec,

May

et al. 2023

Anal. Chem.

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We demonstrate a rapid and sensitive method for DNA detection without the need for fluorescence. This is based on carbon-coated magnetic iron (Fe) microparticles with a covalent surface attachment of DNA. We show that these magnetic microparticles can capture complementary DNA. Significantly, the DNA covalent surface bonds are robust to high temperatures and can be included in a sample during polymerase chain reaction (PCR). This method is employed for the detection of targeted DNA sequences (40–50 bp). Hybridization probes on the surface of the magnetically susceptible Fe microparticle recognize the target DNA sequence-specifically. The double-stranded DNA (dsDNA) microparticles are then quickly captured with a magnet from the sample matrix. This foregoes postpurification processes, such as electrophoresis, which make our technique time- and cost-effective. Captured dsDNA can be detected with intercalating dyes such as ethidium bromide through a loss in the UV absorption signal with a limit of detection (LOD) of 24 nM within 15 min. Likewise, surface-bound DNA can act as a primer in PCR to decrease the LOD to 5 pM within 2 h. This is the first instance of a nucleotide-modified magnetically susceptible carbon substrate that is PCR-compatible. Besides DNA capture, this strategy can eventually be applied to sequence-specific nucleic acid purification and enrichment, PCR cleanup, and single-strand generation. The DNA-coated particles are stable under PCR conditions (unlike commonly used polystyrene or gold particles).

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Section: Resultsmentioning

confidence: 99%

Biosensing with Polymerase Chain Reaction-Stable DNA-Functionalized Magnetically Susceptible Carbon–Iron Microparticles

Koirala,

Dalbec,

May

et al. 2023

Anal. Chem.

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“…The gelation (or sol-gel transition) of polymer solutions is a hot topic of scientific and technological interest [1]. In particular, the preparation of hydrogels is important in the food, cosmetic, pharmaceutical, medical and bioplastics industries; furthermore, it is fundamental in the fabrication of 3D scaffolds for tissue engineering [2][3][4][5]. From the rheological point of view there are many open problems, such as the optimization of the formulation, the control of the aggregation kinetics and the crosslinking of the polymer material [6].…”

Section: Introductionmentioning

confidence: 99%

Viscosity Flow Curves of Agar and the Bounded Ripening Growth Model of the Gelation Onset

Villani

2024

Molecules

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The gelation kinetics of agar aqueous solutions were studied by means of the viscosity flow curves using a coaxial Couette cylinder viscometer. The viscosity curves show an unusual sigmoidal trend or an exponential decay to a viscous steady state. An original theory of gelation kinetics was developed considering the coarsening of increasingly larger and more stable clusters due to Ostwald ripening and the breakup of clusters that were too large due to the instability of rotating large particles induced by the shear rate. The developed Bounded Ripening Growth model takes into account the trend of the viscosity curves by means of an autocatalytic process with negative feedback on aggregation according to the logistic kinetic equation, in which the constants k1(γ) and k−(ν) are governed by the surface tension and shear rate, respectively. A dimensionless equation based on the difference between the Weber number and the ratio of the inverse kinetic constant to forward constant, accounts for the behavior of the dispersed phase in equilibrium conditions or far from the hydrostatic equilibrium.

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“…With strong specificity, high selectivity and sensitivity, convenience, and fast operation, the biosensor detection technology that can realize online addition has been widely used in food safety, such as for detecting food freshness, pathogenic bacteria, pesticide residues, antibiotic residues, heavy metals, and so forth [14]. Recently, due to their highly porous structure, good shape-adaptability, mechanical stability, and high biocompatibility, hydrogels have become a promising biomaterial for designing food safety sensors [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…and transducing elements (electrochemical, optical, thermometric, ion-sensitive, or piezoelectric forms) [19,20]. Combining hydrogel with biometric elements, rapid, sensitive, and portable target-responsive hydrogel-based biosensors have been fabricated to realize target recognition and signal amplification, which can meet the needs of real-time quantitative detection [15,21,22]. Although the above advantages exist, the disadvantages of high price and stability also restrict the application of hydrogels in biosensors to a certain extent.…”

Section: Introductionmentioning

confidence: 99%

Recent Studies and Applications of Hydrogel-Based Biosensors in Food Safety

Li,

Zhang,

et al. 2023

Foods

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Food safety has increasingly become a human health issue that concerns all countries in the world. Some substances in food that can pose a significant threat to human health include, but are not limited to, pesticides, biotoxins, antibiotics, pathogenic bacteria, food quality indicators, heavy metals, and illegal additives. The traditional methods of food contaminant detection have practical limitations or analytical defects, restricting their on-site application. Hydrogels with the merits of a large surface area, highly porous structure, good shape-adaptability, excellent biocompatibility, and mechanical stability have been widely studied in the field of food safety sensing. The classification, response mechanism, and recent application of hydrogel-based biosensors in food safety are reviewed in this paper. Furthermore, the challenges and future trends of hydrogel biosensors are also discussed.

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Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection

Cited by 16 publications

References 183 publications

Biosensing with Polymerase Chain Reaction-Stable DNA-Functionalized Magnetically Susceptible Carbon–Iron Microparticles

Biosensing with Polymerase Chain Reaction-Stable DNA-Functionalized Magnetically Susceptible Carbon–Iron Microparticles

Viscosity Flow Curves of Agar and the Bounded Ripening Growth Model of the Gelation Onset

Recent Studies and Applications of Hydrogel-Based Biosensors in Food Safety

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