CRISPR-Cas gene editing technology and its application prospect in medicinal plants

Guo, Miaoxian; Chen, Hongyu; Dong, Shuting; Zhang, Zheng; Luo, Hongmei

doi:10.1186/s13020-022-00584-w

Cited by 21 publications

(4 citation statements)

References 178 publications

(168 reference statements)

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“… 13 The CRISPR-Cas system consists of a CRISPR array and the Cas gene family, in which the CRISPR array is composed of highly conserved repeat sequences and spacer sequences, with upstream leader sequences responsible for their transcription. 14 The Cas gene family, the CRISPR-related protein genes, is highly conserved, which encodes the Cas protein with a domain capable of cleaving DNA double-strands. 15 In the CRISPR array, the palindromic structure contained in the repeat sequence can form a conserved RNA secondary structure, which can bind to the Cas protein to form a complex and work together.…”

Section: Introductionmentioning

confidence: 99%

Association of CRISPR-Cas System with the Antibiotic Resistance and Virulence Genes in Nosocomial Isolates of Enterococcus

Tao¹,

Chen²,

Li³

et al. 2022

IDR

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Purpose This study aimed to investigate the prevalence of the CRISPR-Cas system in nosocomial isolates of Enterococcus and their possible association with antibiotic resistance and virulence genes. Materials and Methods Identification and antimicrobial susceptibility of the microorganism were performed by the automatized VITEK 2 Compact system (bioMerieux, France). A total of 100 Enterococcus isolates were collected and identified by VITEK 2 Compact automatic microbial identification drug susceptibility analyzer. The prevalence of various CRISPR-Cas systems, antibiotic resistance genes and virulence genes were investigated by polymerase chain reaction (PCR). The prevalence of CRISPR-Cas systems associated with antibiotic resistance and virulence genes was performed by appropriate statistical tests. Results A total of 100 isolates of Enterococcus were identified and there were 62/100(62.0%) Enterococcus faecalis isolates and 38/100(38.0%) Enterococcus faecalis isolates. In total, 46 (46.0%) of 100 isolates had at least one CRISPR-Cas locus. CRISPR elements were more prevalent in Enterococcus faecalis isolates. The results of PCR demonstrated that CRISPR1-Cas, orphan CRISPR2, and CRISPR3-Cas were present in 23 (23.0%), 42 (42.0%) and 5 (5.0%) Enterococcus isolates, respectively. Compared with CRISPR-Casnegative isolates, the CRISPR-Cas positive isolates showed significant lower resistance rates against ampicillin, erythromycin, levofloxacin, tetracycline, vancomycin, gentamicin, streptomycin, and rifampicin. Presumably consistent with drug susceptibility, fewer CRISPR loci were identified in vanA, tetM, ermB, aac6’-aph(2”), aadE , and ant(6) positive isolates. There was a significant negative correlation between the CRISPR-Cas locus and the enterococcal virulence factors enterococcal surface protein ( esp ) gene. Conclusion In conclusion, the results indicated that the absence of the CRISPR-Cas system was negatively associated with some antibiotic resistance in clinical isolates of Enterococcus faecalis and Enterococcus faecium . Also, there was a negative correlation with the carriage of antibiotic resistance genes. Furthermore, CRISPR-Cas may prevent some isolates from acquiring certain virulence factors.

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Section: Introductionmentioning

confidence: 99%

Association of CRISPR-Cas System with the Antibiotic Resistance and Virulence Genes in Nosocomial Isolates of Enterococcus

Tao¹,

Chen²,

Li³

et al. 2022

IDR

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“…The rapid advances in plant genomics, transcriptomics and proteomics, plus the recent emergence of metabolomics and experimental techniques for molecular biology and analytical chemistry, will greatly facilitate and enhance the biosynthesis engineering of medicinal plants. Moreover, synthetic biology, along with gene editing technology ( Guo, Chen, Dong, Zhang, & Luo, 2022 ), is valuable, versatile, and efficient tools for research, development, and commercialization of natural products of medicinal plants.…”

Section: Discussionmentioning

confidence: 99%

Strategies on biosynthesis and production of bioactive compounds in medicinal plants

Guo,

Lv,

Chen

et al. 2024

Chinese Herbal Medicines

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“…In addition, potential CRISPR applications for medicinal plants, such as Salvia miltiorrhiza , Dendrobium officinale , Cannabis sativa , and Opium poppy , have been proposed. However, they are still in the early stages of development [ 125 ].…”

Section: The Rapid Emergence Of the Crispr/cas System For High Specif...mentioning

confidence: 99%

Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World

Hamdan

Noor

Abd-Aziz

et al. 2022

Plants

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Technological applications in agriculture have evolved substantially to increase crop yields and quality to meet global food demand. Conventional techniques, such as seed saving, selective breeding, and mutation breeding (variation breeding), have dramatically increased crop production, especially during the ‘Green Revolution’ in the 1990s. However, newer issues, such as limited arable lands, climate change, and ever-increasing food demand, pose challenges to agricultural production and threaten food security. In the following ‘Gene Revolution’ era, rapid innovations in the biotechnology field provide alternative strategies to further improve crop yield, quality, and resilience towards biotic and abiotic stresses. These innovations include the introduction of DNA recombinant technology and applications of genome editing techniques, such as transcription activator-like effector (TALEN), zinc-finger nucleases (ZFN), and clustered regularly interspaced short palindromic repeats/CRISPR associated (CRISPR/Cas) systems. However, the acceptance and future of these modern tools rely on the regulatory frameworks governing their development and production in various countries. Herein, we examine the evolution of technological applications in agriculture, focusing on the motivations for their introduction, technical challenges, possible benefits and concerns, and regulatory frameworks governing genetically engineered product development and production.

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CRISPR-Cas gene editing technology and its application prospect in medicinal plants

Cited by 21 publications

References 178 publications

Association of CRISPR-Cas System with the Antibiotic Resistance and Virulence Genes in Nosocomial Isolates of Enterococcus

Association of CRISPR-Cas System with the Antibiotic Resistance and Virulence Genes in Nosocomial Isolates of Enterococcus

Strategies on biosynthesis and production of bioactive compounds in medicinal plants

Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World

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