2020
DOI: 10.1101/2020.06.17.157180
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

CRISPR-Cas-amplified urine biomarkers for multiplexed and portable cancer diagnostics

Abstract: Synthetic biomarkers, exogenous probes that generate molecular reporters, represent an emerging paradigm in precision diagnostics with applications across infectious and noncommunicable diseases. In order to achieve their promise, these methods reply on multiplexing strategies to provide tools that are both sensitive and specific. However, the field of synthetic biomarkers has not benefited from molecular strategies such as DNA-barcoding due to the susceptibility of nucleic acids in vivo. Herein, we exploit ch… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
14
0

Year Published

2021
2021
2022
2022

Publication Types

Select...
5

Relationship

0
5

Authors

Journals

citations
Cited by 9 publications
(14 citation statements)
references
References 45 publications
0
14
0
Order By: Relevance
“…The genome editing capacity of the CRISPR/Cas system has been investigated as a novel multiplexing platform [195][196][197][198][199]. Owing to the inherent programmability of Cas proteins [200], collateral activity upon multiple 20-mer barcodes can be extended to report potentially hundreds of orthogonal codes.…”
Section: Crispr/cas Systemmentioning
confidence: 99%
See 1 more Smart Citation
“…The genome editing capacity of the CRISPR/Cas system has been investigated as a novel multiplexing platform [195][196][197][198][199]. Owing to the inherent programmability of Cas proteins [200], collateral activity upon multiple 20-mer barcodes can be extended to report potentially hundreds of orthogonal codes.…”
Section: Crispr/cas Systemmentioning
confidence: 99%
“…Owing to the inherent programmability of Cas proteins [200], collateral activity upon multiple 20-mer barcodes can be extended to report potentially hundreds of orthogonal codes. A proof-of-concept study using CRISPR-Cas12a-mediated barcodes has been applied in the detection of urine biomarkers [197]. Massively multiplexed nucleic acid detection with Cas13-based SHERLOCK detection technology along with microfluidic chips has also been applied to both COVID-19 and other viral infections [201].…”
Section: Crispr/cas Systemmentioning
confidence: 99%
“…This opportunity to challenge current limits of detection has enabled the use of synthetic biomarkers for early disease detection in cancer and other pathological conditions. [ 54–63 ]…”
Section: Identifying Predictive Biomarkers Of T Cell Responsementioning
confidence: 99%
“…Multiplexed detection can be achieved by employing a library of mass‐ or DNA‐barcoded biomarkers to monitor multiple proteases simultaneously. [ 54,58,153 ] Additionally, recent advances in machine learning enable the training of diagnostic classifiers that accurately differentiate experimental groups based on multiplexed urinary outputs. Mac et al.…”
Section: Identifying Predictive Biomarkers Of T Cell Responsementioning
confidence: 99%
See 1 more Smart Citation