CRISPR: A new paradigm of theranostics

Yadav, Neelam; Narang, Jagriti; Chhillar, Anil Kumar; Rana, J. S.

doi:10.1016/j.nano.2020.102350

Cited by 10 publications

(8 citation statements)

References 166 publications

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“…The accompanying nucleic acid chain demonstrates the complementarity with the crRNAs. Therefore, the binding of crRNA to the unique invasive pathogen genome sequence results in the shredding of the target DNA/RNA sequence by Cas enzyme [15]. CRISPR loci are composed of 20-40 bp length repeat sequences, which are interspaced by unique 20-58 bp sequences called spacers resulting from small segments of external genetic material (protospacers) [25,26].…”

Section: Structure Of Crispr/casmentioning

confidence: 99%

“…SpCas9 consists of 1368 amino acids and is classified into various regions, each with a different function [28]. In the CRISPR/Cas system, the Cas9 protein serves as a facilitator for site-specific DNA binding and infectious genetic material cleavage [15].…”

Section: Structure Of Crispr/casmentioning

confidence: 99%

“…Biochemical, physiological, or genetic factors such as bacteria, viruses, or fungus induce mutations in the DNA, resulting in such conditions. Treatments for these genetic diseases have traditionally relied on viral-mediated transgenic expression and RNA interference techniques [15].…”

Section: Introductionmentioning

confidence: 99%

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Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

et al. 2022

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Biomedical researchers have subsequently been inspired the development of new approaches for precisely changing an organism's genomic DNA in order to investigate customized diagnostics and therapeutics utilizing genetic engineering techniques. Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one such technique that has emerged as a safe, targeted, and effective pharmaceutical treatment against a wide range of disease-causing organisms, including bacteria, fungi, parasites, and viruses, as well as genetic abnormalities. The recent discovery of very flexible engineered nucleic acid binding proteins has changed the scientific area of genome editing in a revolutionary way. Since current genetic engineering technique relies on viral vectors, issues about immunogenicity, insertional oncogenesis, retention, and targeted delivery remain unanswered. The use of nanotechnology has the potential to improve the safety and efficacy of CRISPR/ Cas9 component distribution by employing tailored polymeric nanoparticles. The combination of two (CRISPR/Cas9 and nanotechnology) offers the potential to open new therapeutic paths. Considering the benefits, demand, and constraints, the goal of this research is to acquire more about the biology of CRISPR technology, as well as aspects of selective and effective diagnostics and therapies for infectious illnesses and other metabolic disorders. This review advocated combining nanomedicine (nanomedicine) with a CRISPR/Cas enabled sensing system to perform early-stage diagnostics and selective therapy of specific infectious disorders. Such a Nano-CRISPR-powered nanomedicine and sensing system would allow for successful infectious illness control, even on a personal level. This comprehensive study also discusses the current obstacles and potential of the predicted technology.

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Section: Structure Of Crispr/casmentioning

confidence: 99%

Section: Structure Of Crispr/casmentioning

confidence: 99%

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Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

et al. 2022

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“…These methods will strengthen the awareness of researchers toward the gut microbiome and provide novel routes for probiotic bacteria to be studied ( Yadav et al, 2018 ). CRISPR, as a revolutionary genome editing technology has provided a key bioengineering tool for investigating different diseases with great sensitivity, speed, precision, and a comprehension of biological processes ( Yadav et al, 2021 ). However, an extensive strategy to diversifying the genome engineering toolbox is to discover new CRISPR systems.…”

Section: Exploiting Powerful Tools In Pediococcus ...mentioning

confidence: 99%

Biotechnological Innovations and Therapeutic Application of Pediococcus and Lactic Acid Bacteria: The Next-Generation Microorganism

Peter

Qiao

Godspower

et al. 2022

Front. Bioeng. Biotechnol.

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Lactic acid bacteria represent a worthwhile organism within the microbial consortium for the food sector, health, and biotechnological applications. They tend to offer high stability to environmental conditions, with an indicated increase in product yield, alongside their moderate antimicrobial activity. Lack of endotoxins and inclusion bodies, extracellular secretion, and surface display with other unique properties, are all winning attributes of these Gram-positive lactic acid bacteria, of which, Pediococcus is progressively becoming an attractive and promising host, as the next-generation probiotic comparable with other well-known model systems. Here, we presented the biotechnological developments in Pediococcal bacteriocin expression system, contemporary variegated models of Pediococcus and lactic acid bacteria strains as microbial cell factory, most recent applications as possible live delivery vector for use as therapeutics, as well as upsurging challenges and future perspective. With the radical introduction of artificial intelligence and neural network in Synthetic Biology, the microbial usage of lactic acid bacteria as an alternative eco-friendly strain, with safe use properties compared with the already known conventional strains is expected to see an increase in various food and biotechnological applications in years to come as it offers better hope of safety, accuracy, and higher efficiency.

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“…In addition to the above, there is the possibility of interrupting the advance of bacterial resistance to antibiotics, in addition to decreasing the virulence of bacterial isolates that cause infections. The purpose is to always try to help human health, but care must be taken not to cause unwanted alterations in the patient (Escalona-Noguero et al, 2021;Tsou et al, 2021;Yadav et al, 2021).…”

Section: Future Perspectives Of Crispr Casmentioning

confidence: 99%

The Challenge of CRISPR-Cas Toward Bioethics

et al. 2021

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Since determining the structure of the DNA double helix, the study of genes and genomes has revolutionized contemporary science; with the decoding of the human genome, new findings have been achieved, including the ability that humans have developed to modify genetic sequences in vitro. The discovery of gene modification mechanisms, such as the CRISPR-Cas system (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas (CRISPR associated). Derived from the latest discoveries in genetics, the idea that science has no limits has exploded. However, improvements in genetic engineering allowed access to new possibilities to save lives or generate new treatment options for diseases that are not treatable by using genes and their modification in the genome. With this greater knowledge, the immediate question is who governs the limits of genetic science? The first answer would be the intervention of a legislative branch, with adequate scientific advice, from which the logical answer, bioethics, should result. This term was introduced for the first time by Van Rensselaer Potter, who in 1970 combined the Greek words bios and ethos, Bio-Ethik, which determined the study of the morality of human behavior in science. The approach to this term was introduced to avoid the natural tension that results from the scientific technical development and the ethics of limits. Therefore, associating the use of biotechnology through the CRISPR-Cas system and the regulation through bioethics, aims to monitor the use of techniques and technology, with benefits for humanity, without altering fundamental rights, acting with moral and ethical principles.

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CRISPR: A new paradigm of theranostics

Cited by 10 publications

References 166 publications

Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

Biotechnological Innovations and Therapeutic Application of Pediococcus and Lactic Acid Bacteria: The Next-Generation Microorganism

The Challenge of CRISPR-Cas Toward Bioethics

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