Crevicular interleukin‐1β in moderate and severe periodontitis patients and the effect of phase I periodontal treatment

Abstract

Hou L-T, Liu C-M, Rossomando FF: Crevicular interleukin-1 fi in moderate and severe periodontitis patients and the effect of phase I periodontal treatment, J Clin Periodontol 1995: 22: 162-167, © Munksgaard, 1995 Abstract, Interleukin-l/? (IL-l/^), a potent stimulator of bone resorption, has been implicated in the pathogenesis of periodontal destruction. However, the relationship between cytokines and periodontal disease has not been studied sufficiently to allow definitive conclusions. The aims of this study are to investigate crevicular IL-l// and the clinical status of patients with periodontitis and the effect of phase I periodontal therapy on levels of IL-l//, For this study, 130 gingival crevicular fluid (GCF) samples were harvested from non-inflamed (15) and diseased sites (115) in 11 patients with periodontitis. The gingival index (GI) and probing depth (PD) of each site was recorded initially and one month after treatment. The amount of IL-ly9 in the GCF was measured by enzyme-linked immunosorbent assay (ELISA) using an antibody specific for this cytokine. Before treatment, IL-\ji was found in 12 of 15 non-inflamed gingival crevices and in 112 of 115 diseased pockets. The amount of IL-l/i varied from 4.03 to 511.12 pg/site. The average amount of IL-l/i from diseased sites was 3-fold greater than that from non-inflamed sites. Both total amount of IL-l/i and the GCF volume, but not IL-l/? concentration, were found to be correlated, positively, with GI score and PD. After therapy, 63 sites from 7 patients were re-examined, and the amount of IL-1/y in 49 of 63 sites was found to have declined. These data suggest that the amount of crevicular IL-1// is closely associated with periodontal status. This relationship may be valuable in monitoring periodontal disease activity.

An Interleukin-1β (IL-1β) Single-Nucleotide Polymorphism at Position 3954 and Red Complex Periodontopathogens Independently and Additively Modulate the Levels of IL-1β in Diseased Periodontal Tissues

“…The levels of the proinflammatory cytokine IL-1␤ are characteristically increased in diseased periodontal tissues and are thought to be a critical determinant of periodontitis outcome (13,14,16,54). In accordance, our data demonstrate a higher IL-1␤ expression in the periodontal tissues of CP patients compared to that of the C group, but the factors involved in the determination of the IL-1␤ levels in diseased tissues remain unknown.…”

“…The presence of periodontopathogens, such as Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola (called the red complex) and Aggregatibacter actinomycetemcomitans, considered the major etiologic agents in periodontitis (8), triggers the expression of proinflammatory cytokines, such as interleukin-1 (IL-1), which have been associated with the immunopathology of periodontitis (14). IL-1␤ has been particularly studied as a critical determinant of tissue destruction due to its proinflammatory and bone resorptive properties, and indeed, increased levels of IL-1␤ in gingival crevicular fluid were correlated with the severity of periodontal disease (13,16,54).…”

Markers of inflammation in periodontal diseases

“…The concentration of the cytokine decreased after scaling and root surface planing, even if it did not correlate with pocket depth change. Similar results were obtained by Hou et al [11]. They observed that the amount of IL-1β is closely associated with progression of lesions in periodontium.…”

“…The amount of IL-1β was also correlated with clinical parameters [15]. Another study by Hou et al [11] suggested that the amount of IL-1β in GCF is closely associated with the patient's periodontal status. Engebredson et al [16] observed that increased levels of IL-1β in gingival crevicular fluid in patients with severe periodontal disease, is also found in shallow pockets, which confirms the role of the genetic factor associated with an increased expression of IL-1β in the pathogenesis of chronic periodontitis.…”

La IL-1 y su eventual asociación con la enfermedad periodontal crónica: Una revisión de la literatura (I)

“…Wilton y col. (24) tampoco encontraron ningún tipo de correlación entre la concentración de IL-1β en FGC y los parámetros clínicos que estudiaron: profundidad de sondaje, pérdida de inserción e índices de sangrado y placa. Por el contrario Hou y col. (40), observaron que la cantidad total, pero no la concentración de IL-1β en FGC correlaciona tanto con el índice gingival como con la profundidad de sondaje. Así mismo Engebreston y col. (38) también observaron una asociación estadísticamente significativa entre la [IL-1β] en FGC y la profundidad al sondaje, así como también con la pérdida de inserción.…”