CREDO: a new general-purpose laboratory instrument for small-angle X-ray scattering

Wacha, András; Varga, Zoltán; Bóta, Attila

doi:10.1107/s1600576714019918

Cited by 39 publications

(32 citation statements)

References 29 publications

(28 reference statements)

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“…Small-angle X-ray scattering (SAXS) measurements on the cochleate samples were carrried out with a CREDO instrument integrated with a GeniX 3D Cu ULD microfocus system (Xenocs SA, Sassenage, France) for producing X-rays and a Pilatus-300k CMOS hybrid pixel position sensitive detector (Dectris Ltd, Baden, Switzerland) [22,23]. The recorded 2D…”

Section: Small-angle X-ray Scatteringmentioning

confidence: 99%

Dispersion and stabilization of cochleate nanoparticles

Bozó

Wacha

Mihály

et al. 2017

European Journal of Pharmaceutics and Biopharmaceutics

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Cochleates, calcium-stabilized membrane rolls of nanoscale diameter, promise a unique and efficient way of delivering lipid-soluble drugs, proteins or nucleic acids into biological systems because they protect the encapsulated material against enzymatic or chemical degradation. Self-aggregation, which typically arises during production and storage is a major obstacle that has so far precluded the development of an efficient cochleate-based drugdelivery system. Here we show that citric acid, added transiently in a narrow concentration range, effectively disperses cochleate aggregates, stabilizes the disperse state for long-term storage and preserves the canonical ultrastructure and topological characteristics of cochleate nanoparticles.

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Section: Small-angle X-ray Scatteringmentioning

confidence: 99%

Dispersion and stabilization of cochleate nanoparticles

Bozó

Wacha

Mihály

et al. 2017

European Journal of Pharmaceutics and Biopharmaceutics

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“…Small-angle X-ray scattering measurements [20][21][22] were performed using CREDO, an in-house transmission geometry set-up [23]. Samples were filled into thin-walled quartz capillaries of 1.2 mm average outer diameter.…”

Section: Small Angle X-ray Scattering (Saxs)mentioning

confidence: 99%

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Deák

Mihály

Szigyártó

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Colloidal stabile nanoerythrosomes with 200 nm average diameter were formed from hemoglobin-free erythrocyte ghost membrane via sonication and membrane extrusion. The incorporation of extra lipid (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC), added to the sonicated ghosts, caused significant changes in the thermotropic character of the original membranes. As a result of the increased DPPC ratio the chain melting of the hydrated DPPC system and the characteristic small angle X-ray scattering (SAXS) of the lipid bilayers appeared. Significant morphological changes were followed by transmission electron microscopy combined with freeze fracture method (FF-TEM). After the ultrasonic treatment the large entities of erythrocyte ghosts transformed into nearly spherical nanoerythrosomes with diameters between 100-300 nm and at the same time a great number of 10-30 nm large membrane proteins or protein clusters were dispersed in the aqueous medium. The infrared spectroscopy (FT-IR) pointed out, that the sonication did not cause changes in the secondary structures of the membrane proteins under our preparation conditions. About fivefold of extra lipid -compared to the lipid content of the original membrane -caused homogeneous dispersion of nanoerythrosomes however the shape of the vesicles was not uniform. After the addition of about tenfold of DPPC, monoform and monodisperse nanoerythrosomes became typical. The outer surfaces of these roughly spherical objects were frequently polygonal, consisting of a net of pentagons and hexagons..

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“…33 X-rays were supplied by a GeniX 3D Cu ULD integrated beam delivery system (Xenocs SA, Sassenage, France). The highly monochromatic Cu K a radiation (0.15418 nm wavelength) was collimated by three pinholes of sizes 300, 300 and 400 mm diameter.…”

Section: Characterizationmentioning

confidence: 99%

Inherently fluorescent and porous zirconia colloids: preparation, characterization and drug adsorption studies

et al. 2015

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Porous, fluorescent zirconia particles of nearly 380 nm diameter were prepared without template molecules or labeling dyes. The porous structure is the result of aggregation-induced particle formation. The inherent fluorescence is assigned to coordinatively unsaturated Zr 4+ ions at the sol-gel derived ZrO 2 surface. After physico-chemical characterization of the native zirconia particles carboxyl and/or amine bearing drug molecules (D,L-a-difluoromethylornithine -DFMO, ursolic acid -UA and doxorubicin -DOX) were adsorbed onto their surface, and the products were analyzed with Fourier-transform infrared spectroscopy (FTIR), thermogravimetry (TG), small-angle X-ray scattering (SAXS), fluorimetry and zeta potential vs. pH measurements. We have found that DOX complexes coordinatively unsaturated Zr 4+ ions without dislocating them, while carboxyl-bearing drugs interact with basic surface Zr-OH sites eliminating some of the carbonate species. The adsorption of UA at the zirconia surface shifts considerably the isoelectric point of the surface and thus provides kinetic stability to the particles at physiological pH. An in vivo biodistribution study in two healthy dogs performed by SPECT/CT detection after 99mTc labeling of the nanocarriers has shown the possibility of drug delivery application. IntroductionNano-and microparticles of various kinds are currently investigated with the prospect of drug delivery application. The requirements to be fulfilled are numerous: drug carrier particles should encapsulate, transport and release drug molecules in a controlled way in living organism. 1 Multifunctionality is, therefore, inevitable and -when coupled to easy synthesisrepresents a great advantage. Among inorganic particles silica 2,3 is preferred for its controlled synthesis, low toxicity, established conjugation methods and high drug loading capacity (for mesoporous silica).Zirconia, a ceramic material, is used for biomedical applications e.g. in dental and chirurgical implants. [4][5][6] It is known to be biocompatible, and we have shown in our preliminary experiments that zirconia is also biodegradable under certain circumstances. The surface of zirconia is chemically active, having both acidic and basic sites. 7 This explains that zirconia chemisorbs both NH 3 and CO 2 . 8 Under ambient conditions, the zirconia surface is always covered by CO and CO 2 adsorbed from the air. 9,10 Complex formation has also been observed with the carboxyl groups of citric acid and poly-e-caprolactone. 11,12 As was observed by Pokrovski et al. CO 2 adsorption on monoclinic (m-) ZrO 2 is more than an order of magnitude higher than that on tetragonal (t-) ZrO 2 . 13 High-temperature synthesis or annealing of ZrO 2 leads to the transformation of the monoclinic phase into tetragonal. However, the lack of a generally approved synthetic method providing stable m-ZrO 2 colloid hinders its use as a drug carrier. Nanocrystalline powders of t-ZrO 2 are produced by aqueous phase reaction followed by annealing.14-17 These materials sintered by a fi...

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CREDO: a new general-purpose laboratory instrument for small-angle X-ray scattering

Cited by 39 publications

References 29 publications

Dispersion and stabilization of cochleate nanoparticles

Dispersion and stabilization of cochleate nanoparticles

Physicochemical characterization of artificial nanoerythrosomes derived from erythrocyte ghost membranes

Inherently fluorescent and porous zirconia colloids: preparation, characterization and drug adsorption studies

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