Creation of a novel lipid-trehalose derivative showing positive interaction with the cell membrane and verification of its cytoprotective effect during cryopreservation

Yoshida, Kozue; Ono, Fumiyasu; Chouno, Takehiro; Nakada, Shota; Ikegami, Yasuhiro; Shirakigawa, Nana; Sakai, Yusuke; Ijima, Hiroyuki

doi:10.1016/j.jbiosc.2021.03.010

Cited by 8 publications

(8 citation statements)

References 61 publications

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“…It is predominantly used as an adjunct to membrane-penetrating CPAs such as DMSO. Recently, ionic liquids [26] and glycolipids [27] have been reported to have cryoprotective properties, and the search for CPAs from the viewpoint of molecular chemistry using computer simulation has attracted significant interest [28].…”

Section: Polymeric Cryoprotectants-polymer Chemistry and Cryobiologymentioning

confidence: 99%

Bridging polymer chemistry and cryobiology

Matsumura

Rajan

Ahmed

2022

Polym J

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Polymers, especially charged polymers, are the key to a sustainable future, as they have the capability to act as alternatives to plastics, reduce the impact of global warming, and offer solutions to global environmental pollution problems. Biomaterial polymers have proven to be incredibly effective in a multitude of applications, including clinical applications. In the fields of cryobiology and cryopreservation, polymers have emerged as credible alternatives to small molecules and other compounds, yielding excellent results. This review outlines the results of research in the areas of polymer chemistry and cryobiology, which have not been discussed together previously. Herein, we explain how recent polymer research has enabled the development of polymeric cryoprotectants with novel mechanisms and the development of novel methods for the intracellular delivery of substances, such as drugs, using a cryobiological technique called the freeze-concentration effect. Our findings indicate that interdisciplinary collaboration between cryobiologists and polymer chemists has led to exciting developments that will further cell biology and medical research.

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Section: Polymeric Cryoprotectants-polymer Chemistry and Cryobiologymentioning

confidence: 99%

Bridging polymer chemistry and cryobiology

Matsumura

Rajan

Ahmed

2022

Polym J

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“…The trehalose or trehalose lipid molecules located on the cell membranes could efficiently mitigate surfactant-induced cell injury. 37 On the other hand, Tre-C n molecules could insert into the lipid bilayer as the concentration further increased, causing changes in membrane fluidity and even forming micelles to lyse phospholipids or proteins from the cell membrane, thus leading to cell membrane disruption. 52 Thus, the stabilizing effectiveness of Tre-C n could be lost as the concentration was further increased.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Typically, hemolytic activity of biosurfactants occurs according to two concentration-dependent mechanisms: osmotic lysis and membrane solubilization, while CMC is one of the most important factors . Actually, trehalose lipids have a strong interaction with the cell membranes, ,− which was assumed to adsorb on or insert into the membrane bilayers, forming individual mixed micelles . Based on the membrane activity of trehalose lipids, it was supposed that Tre-C n could exert cryoprotection at the concentration range below their CMC values via membrane stabilization of trehalose in either lipid or dissociative structures.…”

Section: Resultsmentioning

confidence: 99%

“…Natural trehalose lipids, known as important glycolipids produced by bacteria, possess various biological and physicochemical properties. , Due to their amphiphilic and surface-active properties, trehalose lipids are able to interact with the lipid membranes of cells. ,− As a sort of biosurfactants, synthetic analogues of trehalose lipids, trehalose fatty acid esters with comparable structures, have been developed as excipients for stabilization of proteins against aggregation, , or vaccine adjuvants for enhanced immunogenicity. , Additionally, a glycolipid derivative of oleyl-trehalose exerted cryoprotective effects on cryopreservation of hepatocytes by interaction with the cell membrane . Inspired by the biosurfactant structures, in this work, trehalose was modified using fatty acids with relatively long alkyl chains to enhance the membrane-binding activity.…”

Section: Introductionmentioning

confidence: 99%

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Integration of Trehalose Lipids with Dissociative Trehalose Enables Cryopreservation of Human RBCs

Wang

Gao

Zhu

et al. 2022

ACS Biomater. Sci. Eng.

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Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures. Trehalose has been considered as a biocompatible cryoprotectant that naturally accumulates in organisms to tolerate anhydrobiosis and cryobiosis. Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization. Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose. Moreover, there were slight changes in cell morphology and cell functions. It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival. The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.

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“…To protect the survivability of LAB during freeze-drying, some cryoprotectants, such as trehalose, alginate, and skim milk (SM), have been commercially applied [12,[20][21][22]. These cryoprotectants with different molecular weights have been shown to protect the viability of microorganisms by inhibiting ice recrystallization, cell membrane protection, and antioxidant activity, as reported in many studies [13,[21][22][23][24]. Among the many cryoprotectant candidates, collagen is a component that can be obtained from fish scales or pig skin and is composed of a variety of molecular sizes [13,25,26].…”

Section: Introductionmentioning

confidence: 99%

Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions

Kim¹,

Kim²,

Kim³

et al. 2021

Fermentation

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The intracellular homeostasis of lyophilized lactic acid bacteria (LAB) is destroyed by extreme cold stress, resulting in decreased stability. This study aimed to verify the validity of collagen as a potential protective agent for improving microbial stability deteriorated by freezing. The collagen types used in this study were low molecular weight collagen (LC) of less than 1000 Da and low molecular weight collagen-peptide (LCP) of less than 300 Da. By the accelerated stability test according to the addition of each collagen type, a 3% LCP displaying a protective effect on the viability of various LAB strains (Lactoplantibacillus plantarum MG989, Lactococcus lactis MG5125, Enterococcus faecium MG5232, Bifidobacterium animalis ssp. lactis MG741, and Streptococcus thermophilus MG5140) was finally selected. It was evaluated whether LCP enhances bacterial stability, survivability in the gastrointestinal (GI) tract, and heat resistance. LCP significantly improved the viability of all strains in the GI tract compared to sucrose and skim milk, which are conventional protective agents. Based on morphological observations, LCP was uniformly coated on the cell surface, resulting in protective effects against multiple external stress stimuli. Such findings indicate the applicability of LCP as an unprecedented protective agent, which can improve the stability of various probiotics with antifreeze effects.

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Creation of a novel lipid-trehalose derivative showing positive interaction with the cell membrane and verification of its cytoprotective effect during cryopreservation

Cited by 8 publications

References 61 publications

Bridging polymer chemistry and cryobiology

Bridging polymer chemistry and cryobiology

Integration of Trehalose Lipids with Dissociative Trehalose Enables Cryopreservation of Human RBCs

Survivability of Collagen-Peptide Microencapsulated Lactic Acid Bacteria during Storage and Simulated Gastrointestinal Conditions

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