Creation of a caecostoma in mice as a model of an enterocutaneous fistula

Abstract

In the past, physiological, oncological and pharmaceutical investigations led to the development of different models of enterocutaneous fistulas. For a few years, fistula models were also created to investigate the efficacy of minimal invasive therapies in closing these fistulas. As most experimental surgical procedures are difficult to perform and are often combined with a high mortality or spontaneous closure rate, a new model in mice was developed with the aim of avoiding these disadvantages. Twenty mice, divided into two groups of 10 animals each, had a caecostoma created surgically. The two groups differed regarding the technique of creating the stoma. All animals survived and gained weight during a postoperative period of 4 months. The operation was easy to perform and lasted no longer than about 30 min. Neither a spontaneous closure of the fistula nor any prior death of animals occurred. In conclusion, because of its simplicity and low complication rate, the presented model of an enterocutaneous fistula may offer an interesting alternative to other models for a variety of indications.

Designing 3D Mesenchymal Stem Cell Sheets Merging Magnetic and Fluorescent Features: When Cell Sheet Technology Meets Image-Guided Cell Therapy

“…There was no complication related to fistula induction surgery. To date, apart from the present study and that of Bultmann and colleagues 31 , no other fistula model, either in mice or in any other animal, has been reported in the literature.…”

“…Animals underwent a surgical cecostoma communicating the cecum and the skin under isoflurane anesthesia (Baxter, Maurepas, France) using a SZX10 stereomicroscope (Olympus France SAS, Rungis, France). The cecostoma was performed according the model described by Bultmann and colleagues and this choice was based on the report of no spontaneous stoma closure even four months after surgery 31 . After a midline laparotomy of 1 cm, the distal part of the cecum was passed through the abdominal muscle and the subcutaneous space, forming a 1-cm-long tract, and then attached to the skin.…”

Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula

“…A surgical cecostomy was performed according the model described by Bültmann and colleagues. 53 Rats were placed in supine position, feet spread apart and their abdomen was shaved. After a midline laparotomy of 3 cm, the distal part of the cecum was passed with a staggered opening through the abdominal muscle and the subcutaneous space, forming a 10 mm-long tract.…”

Evaluation of animal models of Crohn's disease with anal fistula (Review)

“…Ryska et al (12) studied rats using a modified cecal anastomosis described by Bültmann et al (13). Ryska et al (12) performed the following operations on rats: The animals underwent a short (2-2.5 cm) median laparotomy following back fixation.…”