Evaluation of animal models of Crohn's disease with anal fistula (Review)

Abstract

Anal fistula is a common and serious complication of Crohn's disease (CD). A sufficiently suitable animal model that may be used to simulate this disease is yet to be established. The aim of the present review was to summarize the different characteristics and experimental methods of commonly used animal models of CD with anal fistula. Electronic databases were searched for studies reporting on the use of this type of animal model. A total of 234 related articles were retrieved, of which six articles met the inclusion criteria; these were used as references for the present review article. The characteristics of the animal models, the advantages and disadvantages of the modeling methods and the similarities with patients with CD and anal fistula were summarized and analyzed. The evidence suggests that a sufficiently suitable animal preclinical model requires to be established.

An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease

“…At present, the only existing animal models include SAMP1/Yit mice, canine anal furunculosis (CAF), or TNBS rats with surgical creation of transsphincteric fistula ( 9 ). However, less than 6% of SAMP1/Yit mice develop fistulas, and the latter rat model is not relevant for the study of fistula formation ( 9 , 10 ).…”

Bridging the Research Gap: Establishing a Rabbit Model for Perianal Fistula (Nassor-E Maqad) Study

“…The surgical induction of the Fistula-in-ano model involves a meticulously orchestrated series of procedures [7][8][9][10][11], including anesthesia administration, aseptic preparation, creation of a perianal skin incision, mucosal dissection, and placement of a foreign body to induce a persistent stula tract.…”

Catching Them Early: Framework Parameters and Progress for Prenatal and Childhood Application of Advanced Therapies

“…These differences concern cell expansion behavior, properties after immortalization or cryopreservation, and choices of paracrine signaling molecules [140,141]; moreover, conclusions across multiple studies are exacerbated by differences between alternative models for the same disease [142]. Therefore, with the swift elimination of human MSCs in immunocompetent mice, xenograft studies based on immunodeficient mice or studies in species for which MSCs or the relevant anatomy more closely reflect human biology [143,144] appear to be the most informative. Based on adolescent to young adult (6-8-week-old) mice, the latest findings for immune and inflammatory disorders include the identification of therapeutic antioxidant and pro-angiogenic action of placenta-derived human MSCs in a surgical model for Crohn's-like enterocutaneous fistula [145].…”