Creatine synthesis and transport during rat embryogenesis: Spatiotemporal expression of AGAT, GAMT and CT1

Braissant, Olivier; Henry, Hugues; Villard, Anne-Marie; Speer, Oliver; Wallimann, Theo; Bachmann, C.

doi:10.1186/1471-213x-5-9

Cited by 110 publications

(47 citation statements)

References 52 publications

(65 reference statements)

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“…Thus, the alanine determination was less suitable than the determination of glutamine in cell media for cellular differentiation monitoring. An increased level of amino acid (both essential and nonessential) in the media could be regarded as a sign of the completion of cellular differentiation and the activation of programmed cell death processes which are typical for highly differentiated C2C12 myoblasts (Burattini et al, 2004). In the present study, we showed that the release of arginine derivatives may be determined with greater reliability than the consumption of starting amino acid from the medium, including arginine.…”

Section: Discussionsupporting

confidence: 51%

Homoarginine and ornithine production during C2C12 myogenic differentiation

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Prikhodko

et al. 2018

BioComm

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Assessment of cellular rates of amino acid consumption and release in vitro allows the study of cell culture in a time-course experiment without any cell damage. Determination of the release of amino acid metabolites that initially were not present in the media provides more reliable information about the processes of growth and differentiation in comparison with determination of amino acid consumption rates. Homoarginine (hArg), a derivative of arginine, is generated as the minor product in the reaction catalyzed by L-Arginine: glycine amidinotransferase, where L-lysine serves as an acceptor for amidine group instead of glycine. Ornithine is another product generated in this reaction from arginine. Thus, the goal of the present study was to evaluate the rate of hArg and ornithine accumulation in comparison to the rate of consumption of other amino acids in the course of C2C12 myoblast differentiation. The release time profiles were similar for hArg and ornithine, with the maximum corresponding to the second day of differentiation. The shift for hArg at this time point was detected with greater reliability (p < 0.002) than for ornithine and other amino acids. We suggest that hArg and ornithine could serve as markers to monitor the processes of myoblasts growth and differentiation.

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Section: Discussionsupporting

confidence: 51%

Homoarginine and ornithine production during C2C12 myogenic differentiation

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Subbotina

Prikhodko

et al. 2018

BioComm

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“…We also observed a decrease in NAA in the parietal WM of infants with pWMLs. A product of mitochondrial metabolism in neurons, and possibly, pre-myelinating oligodendrocytes, NAA is often used as a biomarker to estimate the extent of cell damage or death [49], [50], [51], [52], [53], [54]. Accordingly, the elevation in glutamine coupled with a decrease in NAA in our cases with pWMLs may reflect damage or loss of axons and pre-myelinating oligodendrocytes in the subacute phase of injury, as shown in neuropathology studies of PVL [5], [55], [56].…”

Section: Discussionmentioning

confidence: 99%

Altered Glutamatergic Metabolism Associated with Punctate White Matter Lesions in Preterm Infants

et al. 2013

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Preterm infants (∼10% of all births) are at high-risk for long-term neurodevelopmental disabilities, most often resulting from white matter injury sustained during the neonatal period. Glutamate excitotoxicity is hypothesized to be a key mechanism in the pathogenesis of white matter injury; however, there has been no in vivo demonstration of glutamate excitotoxicity in preterm infants. Using magnetic resonance spectroscopy (MRS), we tested the hypothesis that glutamate and glutamine, i.e., markers of glutamatergic metabolism, are altered in association with punctate white matter lesions and “diffuse excessive high signal intensity” (DEHSI), the predominant patterns of preterm white matter injury. We reviewed all clinically-indicated MRS studies conducted on preterm infants at a single institution during a six-year period and determined the absolute concentration of glutamate, glutamine, and four other key metabolites in the parietal white matter in 108 of those infants after two investigators independently evaluated the studies for punctate white matter lesions and DEHSI. Punctate white matter lesions were associated with a 29% increase in glutamine concentration (p = 0.002). In contrast, there were no differences in glutamatergic metabolism in association with DEHSI. Severe DEHSI, however, was associated with increased lactate concentration (p = 0.001), a marker of tissue acidosis. Findings from this study support glutamate excitotoxicity in the pathogenesis of punctate white matter lesions, but not necessarily in DEHSI, and suggest that MRS provides a useful biomarker for determining the pathogenesis of white matter injury in preterm infants during a period when neuroprotective agents may be especially effective.

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“…For many years, cerebral creatine was believed to be principally of peripheral origin [39], but the works of Braissant et al showed that glycineamidinotransferase and guanidinoacetate methyltransferase, two enzymes necessary for creatine synthesis and function, were also expressed in the brain and, therefore, the brain was shown to be capable of its own creatine synthesis [40, 41]. However, in spite of brain synthesis of creatine, according to Tachikawa et al [42], the brain barriers might be key determinants of the levels of guanidine compounds, including creatine, in the brain and cerebrospinal fluid.…”

Section: Discussionmentioning

confidence: 99%

Synchrotron radiation Fourier-transform infrared and Raman microspectroscopy study showing an increased frequency of creatine inclusions in the rat hippocampal formation following pilocarpine-induced seizures

et al. 2011

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In the present work, synchrotron radiation Fourier-transform infrared (SRFTIR) and Raman microspectroscopies were used to evaluate a possible role of creatine in the pathogenesis and progress of pilocarpine-evoked seizures and seizure-induced neurodegenerative changes in the rat hippocampal tissue. The main goal of this study was to identify creatine deposits within the examined brain area, to analyze their frequency in epileptic animals and naive controls and to examine correlations between the number of inclusions in the hippocampal formation of epileptic rats and the quantitative parameters describing animal behavior during 6-h observation period after pilocarpine injection. The presence of creatine in the brain tissue was confirmed based on the vibrational bands specific for this compound in the infrared and Raman spectra. These were the bands occurring at the wavenumbers around 2800, 1621, 1398, and 1304 cm−1 in IR spectra and around 1056, 908 and 834 cm−1 in the Raman spectra. Creatine was detected in eight of ten analyzed epileptic samples and in only one of six controls under the study. The number of deposits in epileptic animals varied from 1 to 100 and a relative majority of inclusions were detected in the area of the Dentate Gyrus and in the multiform hippocampal layer. Moreover, the number of creatine inclusions was positively correlated with the total time of seizure activity.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-011-5488-z) contains supplementary material, which is available to authorized users.

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Creatine synthesis and transport during rat embryogenesis: Spatiotemporal expression of AGAT, GAMT and CT1

Cited by 110 publications

References 52 publications

Homoarginine and ornithine production during C2C12 myogenic differentiation

Homoarginine and ornithine production during C2C12 myogenic differentiation

Altered Glutamatergic Metabolism Associated with Punctate White Matter Lesions in Preterm Infants

Synchrotron radiation Fourier-transform infrared and Raman microspectroscopy study showing an increased frequency of creatine inclusions in the rat hippocampal formation following pilocarpine-induced seizures

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