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Background and Objectives: Physical activity, recreation and walks successfully counteract negative symptoms of stress in people, especially in large cities, and have many positive psychological and physiological effects. There are many studies showing that contact with nature plays an important role in the regeneration of the human body. The city is not without green enclaves such as forests, parks or greenery along the streets. However, it is not entirely clear how the different physical characteristics of the urban space affect mood improvement, increase of positive feelings, vitality level, etc. Materials and Methods: In the study, two urban environments (apartment and green suburbs) were used, as well as two forests (coniferous and deciduous) to measure the impact of these environments on human physiological and psychological relaxation during a walk in a randomized experiment. The participants of the experiment were 75 young adult Poles studying in the largest Polish agglomeration, Warsaw. Before each experiment, the physiological and psychological state of the participant was measured indoors (pre-test). Four psychological questionnaires were used in the project (Profile of Mood States; Positive and Negative Affect Schedule; Restorative Outcome Scale; Subjective Vitality Scale), and physiological measurements (heart rate, blood pressure) before and after the short walking program were evaluated. Results: As a result of the analyses, it was shown that both staying in an urban environment with greenery and staying in a forest environment have a positive effect on the physiological and psychological relaxation of the subjects. A short walk in the suburbs was no less attractive than a walk in the forest in fall. The above indicates that various places with urban vegetation can be successfully used for recreation, just as in a forest where forest bathing is practiced. This indicates that different places with urban greenery can be successfully used for recreation, as can the forests where forest bathing is carried out.

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Changes in the distribution of proliferating astrocytes expressing glial fibrillary acidic protein (GFAP) and/or vimentin were examined in the injured cerebral hemisphere in adult mice. The injury was followed by [3H]thymidine injections at different time intervals. The brain sections were doubly immunostained for GFAP and vimentin and subjected to autoradiography. In that way three cell types were distinguished immunocytochemically: (1) astrocytes co-expressing glial fibrillary acidic protein (GFAP) and vimentin, (2) astrocytes expressing only GFAP and (3) astrocyte-like cells expressing vimentin. Thereafter, numbers of immunopositive and autoradiographically labelled cells and their locations within the region of injury were recorded at each stage of the experiment. Two hours as well as 1 day after the injury proliferation of GFAP-positive astrocytes and of those co-expressing GFAP and vimentin could only be seen as statistically insignificant phenomena. On day 2 the reactive proliferation of each immunocytochemically defined cell type was already maximal, then gradually decreased and its last signs were recorded on day 8. On day 2, among all the proliferating GFAP-positive astrocytes, 67.2% were also vimentin-positive. Later, the proportion declined to 50.7% and 38.5% on days 4 and 8, respectively. The labelled astrocyte-like vimentin-positive cells were located closest to the lesion margins. In comparison, the astrocytes co-expressing GFAP and vimentin and those expressing exclusively GFAP, occupied regions progressively farther from the lesion site. At the initial stages of the response to injury, vimentin expression in cells starting their reactive proliferation did not precede the expression of GFAP.(ABSTRACT TRUNCATED AT 250 WORDS)