Creatine supplementation does not decrease oxidative stress and inflammation in skeletal muscle after eccentric exercise

Abstract

Thirty-six male rats were used; divided into 6 groups (n = 6): saline; creatine (Cr); eccentric exercise (EE) plus saline 24 h (saline + 24 h); eccentric exercise plus Cr 24 h (Cr + 24 h); eccentric exercise plus saline 48 h (saline + 48 h); and eccentric exercise plus Cr 48 h (Cr + 48 h). Cr supplementation was administered as a solution of 300 mg · kg body weight(-1) · day(-1) in 1 mL water, for two weeks, before the eccentric exercise. The animals were submitted to one downhill run session at 1.0 km · h(-1) until exhaustion. Twenty-four and forty-eight hours after the exercise, the animals were killed, and the quadriceps were removed. Creatine kinase levels, superoxide production, thiobarbituric acid reactive substances (TBARS) level, carbonyl content, total thiol content, superoxide dismutase, catalase, glutathione peroxidase, interleukin-1b (IL-1β), nuclear factor kappa B (NF-kb), and tumour necrosis factor (TNF) were analysed. Cr supplementation neither decreases Cr kinase, superoxide production, lipoperoxidation, carbonylation, total thiol, IL-1β, NF-kb, or TNF nor alters the enzyme activity of superoxide dismutase, catalase, and glutathione peroxides in relation to the saline group, respectively (P < 0.05). There are positive correlations between Cr kinase and TBARS and TNF-α 48 hours after eccentric exercise. The present study suggests that Cr supplementation does not decrease oxidative stress and inflammation after eccentric contraction.

Acute effects of taurine on sarcoplasmic reticulum Ca2+ accumulation and contractility in human type I and type II skeletal muscle fibers

“…In summary, taurine potentiates the rate of SR Ca 2ϩ uptake in both type I and type II human fibers, possibly via an action from within the SR lumen, with the degree of potentiation being significantly reduced at low physiological taurine levels. contractile apparatus; taurine; sarcoplasmic reticulum; single fiber TAURINE IS A SMALL ORGANIC acid found in particularly high concentrations in cardiac and skeletal muscle and is thought to be involved in numerous important processes such as modulating osmotic pressure, cation homeostasis, ion channel activity, proinflammatory mediation, protein stabilization, and acting as an antioxidant, to name a few (6,19,21,40,42). Given its many roles, it is not surprising that taurine deficiency can lead to pathogenesis in both cardiac and skeletal muscle (6,21,40).…”

The effects of strength, aerobic, and concurrent exercise on skeletal muscle damage in rats

“…As expected, the exercise acted as a potent oxidative stress inducer, and they observed a decrease in the reduced SH, which may indicate a state of redox imbalance or protein damage. 24 Surprisingly, we found an increase in reduced SH content at 24 h post-exercise in the AE and CE groups compared with the SE group. In addition, the AE group had an increase in SH content 48 h post-exercise, significantly different from all the other groups.…”

“…15 SH group oxidation may modify the functional state of a protein, impairing its functioning. 15 Silva et al 24 evaluated the effect of an acute session of 90 min of downhill treadmill running on oxidative stress in the quadriceps muscles of male rats. As expected, the exercise acted as a potent oxidative stress inducer, and they observed a decrease in the reduced SH, which may indicate a state of redox imbalance or protein damage.…”

Phase IV Prospective Clinical Study to Evaluate the Effect of Taurine on Liver Function in Postsurgical Adult Patients Requiring Parenteral Nutrition

“…Taurine-containing nutritional supplements have been shown to reduce markers of inflammation in healthy individuals. [41][42][43] It is possible that the dose of taurine in the studied PN solution is not sufficient to exert adequate anti-inflammatory effects in patients with inflammation.…”