Creatine-Phosphokinase Activity in Semen

Rosalki, S B

doi:10.1016/s0140-6736(66)93060-1

Cited by 7 publications

(11 citation statements)

References 7 publications

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“…According to our results the mean seminal plasma CK activity in the studied population averaged 292.8 U/l: This value is 4.2 times higher compared to the upper normal serum activity of 70 U/l, and suggests local production of the enzyme in the male genital system. Previous results reported in the literature concerning the total seminal plasma CK activity were contradictory (Lehmann & Griffiths 1963;Rosalki 1966;Suyama et al 1968; Bosirom & Anderson 1971; Soufir 1979), probably as a result of the different methods used. It must be emphasized in this connection that human seminal plasma contains a phmpharase which splits the phosphate bond of phosphocreatine, and thus renders en-k assay^ based on the determination of creatine formed from phosphrxreatine unsuitable (Rosalti 1966; Suyama et al 1968).…”

Section: Discussionmentioning

confidence: 93%

Creatine kinase in seminal plasma of infertile men: Activity and isoenzymes

et al. 1981

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Creatine kinase (CK) activity was determined in the seminal plasma of 169 men divided in a) 11 groups according to etiological diagnosis of infertility and b) 2 groups on the basis of the normal or abnormal spermiogram. Electrophoretic separation of CK isoenzymes on agarose gel was also performed. We found that: 1) in seminal plasma enzyme activity is 4.2 times higher than the upper limit in normal serum, 2) CK activity in seminal plasma is exclusively due to the isoenzyme BB (CK1). 3) there is a statistically significant difference of the enzyme levels between men with normal spermiogram and oligo-asthenozoo-spermics, yet with wide overlapping of the individual values, 4) there is no correlation between CK activity and the variables of the spermiogram, as well as between enzyme activity and fructose or acid phosphatase in seminal plasma.

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Section: Discussionmentioning

confidence: 93%

Creatine kinase in seminal plasma of infertile men: Activity and isoenzymes

et al. 1981

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“…These differences in results may be due to differences in the sperm purification method. We used a combination of continuous-step density gradient centrifugation with an inner column [12,13] and modified swim-up for purification of sperm. Using ACP, which is present only in seminal plasma, as an indicator, the seminal plasma residual rate in sperm suspension was calculated.…”

Section: Discussionmentioning

confidence: 99%

“…Continuous-step density gradient centrifugation with an inner column. To a whole volume of sperm, 4.0 mg/mL of albumin with 20 mM Hepes-buffered Hanks' solution (Hanks' solution) was added and filtered through a 40-µm nylon mesh filter (filtered sperm) [12,13]. On 5 mL of isotonized 80% Percoll solution (pH 7.4; Percoll solution) supplemented with 20 mg/mL of albumin, 2 mL of filtered sperm was layered.…”

Section: Methodsmentioning

confidence: 99%

“…Some investigators report that both CK-BB and CK-MiMi are present in sperm [7], while other investigators report that CK-BB and CK-MM are present [8][9][10]. Since CK-BB is present in seminal plasma in a much higher amount than in sperm [11][12][13][14], in the conventional reports sperm and seminal plasma were not well separated and therefore it was highly propable that isoform (CK-BB) of seminal plasma was determined together with that of sperm. We developed a method to purifiy sperm specimen free from seminal plasma using the activity of acid phosphatase (ACP), which is contained only in seminal plasma, as an indicator.…”

Section: Introductionmentioning

confidence: 99%

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Creatine Kinase Isoforms in the Seminal Plasma and the Purified Human Sperm

Miyaji

Kaneko

Ishikawa

et al. 2001

Archives of Andrology

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“…2.7.3.2) in human semen [6, 71 were contradictory: Lehmann and Griffiths [6] indicated the presence of elevated CK activity in seminal fluid, while Rosalki [7] indicated that seminal plasma shows little or no CK activity. The discrepancy between these results appears to be due to the differing methods of determination used.…”

Section: Introductionmentioning

confidence: 99%

Seminal Plasma Creatine Kinase Activity in Fertility Studies

Buitrago¹,

Miralles²,

Muńoz

et al. 1980

Archives of Andrology

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Creatine kinase activity was measured in the seminal fluid of 70 males undergoing fertility studies. The average value obtained at 30°C was 333 f 197 IUil (average ? SD). There was no evidence of a significant correlation between creatine kinase enzymatic activity and density, active lineal motility, or number of normal motile spermatozoa; nor was there any correlation seen between creatine kinase activity and concentration of citric acid, (indicator of prostatic function) or fructose, (indicator of seminal vesicle function). Nonetheless, in two cases of excretory azoospermia, very high values of creatine kinase (2510 and 1280 IU/I) and citric acid levels (4000 and 3000 mg/dl) were observed. These would indicate that the origin of creatine kinase is fundamentally prostatic, in contradiction to previous studies by other authors. In this sense, creatine kinase would be a parameter of prostatic function in studies of male fertility. In two cases of secretory azoospermia due to cryptorchidism and in several very severe oligoasthenozoospermias, low creatine kinase levels were seen, while fructose and citric acid levels were normal. This would give relative value to creatine kinase as an indicator of spermatogenesis.

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Creatine-Phosphokinase Activity in Semen

Cited by 7 publications

References 7 publications

Creatine kinase in seminal plasma of infertile men: Activity and isoenzymes

Creatine kinase in seminal plasma of infertile men: Activity and isoenzymes

Creatine Kinase Isoforms in the Seminal Plasma and the Purified Human Sperm

Seminal Plasma Creatine Kinase Activity in Fertility Studies

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