Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus

Srinivas, Shankar; Watanabe, Tomoko; Lin, Chyuan‐Sheng; William, Chris M; Tanabe, Yukito; Jessell, Thomas M.; Costantini, Frank

doi:10.1186/1471-213x-1-4

Cited by 2,769 publications

(1,553 citation statements)

References 28 publications

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“…Further, no Cre activity could be detected after selection of cells expressing B-cell markers (CD19, B220, immunoglobulin (Ig)M, IgD or IgG). These results with Lck-Cre were confirmed by flow cytometry using the ROSA-YFP lox-STOP deletion (Srinivas et al, 2001) as a fluorescent gene reporter assay (L Drynan and THR unpublished). CD19-Cre expression is limited to B cells, (Figure 3e).…”

Section: Mll-af4 Invertor Mouse Model M Metzler Et Almentioning

confidence: 74%

A conditional model of MLL-AF4 B-cell tumourigenesis using invertor technology

et al. 2006

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MLL-AF4 fusion is the most common consequence of chromosomal translocations in infant leukaemia and is associated with a poor prognosis. MLL-AF4 is thought to be required in haematopoietic stem cells to elicit leukaemia and may be involved in tumour phenotype specification as it is only found in B-cell tumours in humans. We have employed the invertor conditional technology to create a model of MLL-AF4, in which a floxed AF4 cDNA was knocked into Mll in the opposite orientation for transcription. Cell-specific Cre expression was used to generate Mll-AF4 expression. The mice develop exclusively B-cell lineage neoplasias, whether the Cre gene was controlled by B-or T-cell promoters, but of a more mature phenotype than normally observed in childhood leukaemia. These findings show that the MLL-AF4 fusion protein does not have a mandatory role in multi-potent haematopoietic stem cells to cause cancer and indicates that MLL-AF4 has an instructive function in the phenotype of the tumour.

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Section: Mll-af4 Invertor Mouse Model M Metzler Et Almentioning

confidence: 74%

A conditional model of MLL-AF4 B-cell tumourigenesis using invertor technology

et al. 2006

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“…Mice with Lyve1‐cre –/– ;Wls flox/flox or Lyve1‐cre +/– ;Wls –/– genotypes were used as littermate controls (EC‐Wls‐CON). Lyve1‐cre mice were also bred to ROSA26‐Stop flox/flox ‐enhanced yellow fluorescent protein (EYFP) mice to label cells effectively targeted by Lyve1‐cre 16. β‐catenin‐LKO mice were used for microdissection and are described elsewhere 6…”

Section: Methodsmentioning

confidence: 99%

Endothelial Wnts regulate β‐catenin signaling in murine liver zonation and regeneration: A sequel to the Wnt–Wnt situation

Preziosi

Okabe

Poddar

et al. 2018

Hepatology Communications

105

156

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β‐Catenin in hepatocytes, under the control of Wnts, regulates pericentral gene expression. It also contributes to liver regeneration (LR) after partial hepatectomy (PH) by regulating cyclin‐D1 gene expression as shown in the β‐catenin and Wnt coreceptors low‐density lipoprotein receptor‐related protein 5/6 conditional knockouts (KO). However, conditional deletion of Wntless (Wls), required for Wnt secretion, in hepatocytes, cholangiocytes, or macrophages lacked any impact on zonation, while Wls deletion in macrophages only marginally affected LR. Here, we address the contribution of hepatic endothelial cells (ECs) in zonation and LR by characterizing EC‐Wls‐KO generated by interbreeding Wls‐floxed and lymphatic vessel endothelial hyaluronan receptor (Lyve1)‐cre mice. These mice were also used to study LR after PH. While Lyve1 expression in adult liver is limited to sinusoidal ECs only, Lyve1‐cre mice bred to ROSA26‐Stopflox/flox‐enhanced yellow fluorescent protein (EYFP) mice showed EYFP labeling in sinusoidal and central vein ECs. EC‐Wls‐KO mice showed decreased liver weights; lacked glutamine synthetase, cytochrome P450 2e1, and cytochrome P450 1a2; and were resistant to acetaminophen‐induced liver injury. After PH, EC‐Wls‐KO showed quantitative and qualitative differences in cyclin‐D1 expression at 24‐72 hours, which led to a lower hepatocyte proliferation at 40 hours but a rebound increase by 72 hours. ECs and macrophages isolated from regenerating livers at 12 hours showed significant up‐regulation of Wnt2 and Wnt9b messenger RNA; these are the same two Wnts involved in baseline β‐catenin activity in pericentral hepatocytes. Conclusion: At baseline, ECs secrete Wnt proteins essential for β‐catenin activation in pericentral hepatocytes. During LR, sinusoidal and central vein ECs and secondarily macrophages secrete Wnt2, while predominantly central vein ECs and secondarily macrophages are the likely source of Wnt9b. This process spatiotemporally regulates β‐catenin activation in hepatocytes to induce cell proliferation. (Hepatology Communications 2018;2:845‐860)

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“…All experiments were performed according to institutional guidelines approved by the ethics committee of the Hospital Universitario Virgen del Rocio and the European Community (Council Directive 86/609/EEC). Pv Cre (Hippenmeyer et al, 2005), Chat Cre (Rossi et al, 2011), Smo Flox (Long, Zhang, Karp, Yang, & McMahon, 2001), Shh Flox (Lewis et al, 2001), Rosa26R‐Tomato (Madisen et al, 2010), and Rosa26R‐YFP mice (Srinivas et al, 2001) were obtained from Jackson Laboratory (Stocks numbers 8,069, 6,410, 4,526, 4,293, 7,909, and 6,148) and were maintained on their genetic background (B6;129P2, C57BL/6, 129X1/SvJ, and C57BL/6J). Breeding previous lines, we generated Pv Cre/+ ; Smo loxP/loxP (PV‐Smo), Chat Cre/+ ; Smo loxP/loxP (ChAT‐Smo), Pv Cre/+ ; Shh loxP/loxP (PV‐Shh), and Chat Cre/+ ; Shh loxP/loxP (ChAT‐Shh).…”

Section: Methodsmentioning

confidence: 99%

Substantia nigra dopaminergic neurons and striatal interneurons are engaged in three parallel but interdependent postnatal neurotrophic circuits

Sanchez-Garcia

Nieto-González

García-Junco-Clemente

et al. 2018

Aging Cell

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The striatum integrates motor behavior using a well‐defined microcircuit whose individual components are independently affected in several neurological diseases. The glial cell line‐derived neurotrophic factor (GDNF), synthesized by striatal interneurons, and Sonic hedgehog (Shh), produced by the dopaminergic neurons of the substantia nigra (DA SNpc), are both involved in the nigrostriatal maintenance but the reciprocal neurotrophic relationships among these neurons are only partially understood. To define the postnatal neurotrophic connections among fast‐spiking GABAergic interneurons (FS), cholinergic interneurons (ACh), and DA SNpc, we used a genetically induced mouse model of postnatal DA SNpc neurodegeneration and separately eliminated Smoothened (Smo), the obligatory transducer of Shh signaling, in striatal interneurons. We show that FS postnatal survival relies on DA SNpc and is independent of Shh signaling. On the contrary, Shh signaling but not dopaminergic striatal innervation is required to maintain ACh in the postnatal striatum. ACh are required for DA SNpc survival in a GDNF‐independent manner. These data demonstrate the existence of three parallel but interdependent neurotrophic relationships between SN and striatal interneurons, partially defined by Shh and GDNF. The definition of these new neurotrophic interactions opens the search for new molecules involved in the striatal modulatory circuit maintenance with potential therapeutic value.

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Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus

Cited by 2,769 publications

References 28 publications

A conditional model of MLL-AF4 B-cell tumourigenesis using invertor technology

A conditional model of MLL-AF4 B-cell tumourigenesis using invertor technology

Endothelial Wnts regulate β‐catenin signaling in murine liver zonation and regeneration: A sequel to the Wnt–Wnt situation

Substantia nigra dopaminergic neurons and striatal interneurons are engaged in three parallel but interdependent postnatal neurotrophic circuits

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