Cranial vasculature in zebrafish forms by angioblast cluster-derived angiogenesis

Proulx, Kira; Lu, Annie; Sumanas, Saulius

doi:10.1016/j.ydbio.2010.08.036

Cited by 162 publications

(170 citation statements)

References 41 publications

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“…Fish (Danio rerio) were maintained at 28.5°C, as previously described by Westerfield (75). Established lines used included WIK, casper (76), cloche (clo m39 ) (70), mindbomb (mib ta52b ) (30), Tg(kdrl:GFP) (10), Tg(kdrl:mCherry) (58), and Tg(wt1b:GFP) (56), where GFP is green fluorescent protein. Embryos were staged according to Kimmel et al (33) and anesthetized with 40 g/ml tricaine methanesulfonate.…”

Section: Methodsmentioning

confidence: 99%

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Rider

Mullins

Verdon

et al. 2015

American Journal of Physiology-Renal Physiology

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Rider SA, Mullins LJ, Verdon RF, MacRae CA, Mullins JJ. Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium. Am J Physiol Renal Physiol 309: F531-F539, 2015. First published July 22, 2015 doi:10.1152/ajprenal.00247.2015.-Although renin is a critical regulatory enzyme of the cardiovascular system, its roles in organogenesis and the establishment of cardiovascular homeostasis remain unclear. Mammalian renin-expressing cells are widespread in embryonic kidneys but are highly restricted, specialized endocrine cells in adults. With a functional pronephros, embryonic zebrafish are ideal for delineating the developmental functions of renin-expressing cells and the mechanisms governing renin transcription. Larval zebrafish renin expression originates in the mural cells of the juxtaglomerular anterior mesenteric artery and subsequently at extrarenal sites. The role of renin was determined by assessing responses to renin-angiotensin system blockade, salinity variation, and renal perfusion ablation. Renin expression did not respond to renal flow ablation but was modulated by inhibition of angiotensin-converting enzyme and altered salinity. Our data in larval fish are consistent with conservation of renin's physiological functions. Using transgenic renin reporter fish, with mindbomb and cloche mutants, we show that Notch signaling and the endothelium are essential for developmental renin expression. After inhibition of angiogenesis, renin-expressing cells precede angiogenic sprouts. Arising from separate lineages, but relying on mutual interplay with endothelial cells, renin-expressing cells are among the earliest mural cells observed in larval fish, performing both endocrine and paracrine functions. renin; zebrafish; notch; endothelium; angiogenesis RENIN is the rate-limiting enzyme of the renin-angiotensin system (RAS). Mammalian ANG II, the effector of the RAS, is principally involved in blood pressure homeostasis by regulation of vasomotor tone and Na ϩ retention. ANG II also regulates cell proliferation and angiogenesis (12,22). Pathological activation of the RAS is associated with cardiovascular diseases, including hypertension and heart failure, and is consequently a major target of therapeutic agents (11).Renin and its cognate cells are required for normal renal development (2), including angiogenesis of the renal vascular tree (59). In mice, ablation of the renin gene or renin-expressing cells leads to renal developmental abnormalities (55,71,79). The vasculature of mammalian embryonic kidneys have a transient but extensive coverage of mural renin-expressing cells (17,31,32,47,63); however, their function is unclear. Adult renin-expressing cells are restricted to the juxtaglomerular apparatus and play a vital role in ion homeostasis by secreting activated renin enzyme via cytoplasmic granules (17,31,36,63). Upon physiological challenge by RAS inhibition or low Na ϩ , prearteriolar vascular smooth muscle cells (VSMC) reestablish their embryonic re...

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Section: Methodsmentioning

confidence: 99%

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Rider

Mullins

Verdon

et al. 2015

American Journal of Physiology-Renal Physiology

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“…Blood vessel development in zebrafish As in other vertebrate clades, angioblasts in zebrafish are derived from the lateral plate mesoderm. These angioblasts differentiate into ECs, which form the bulk of the embryonic blood vessels, although it has been recently observed that some cranial zebrafish vessels form from ECs that differentiate from head angioblast clusters (Proulx et al, 2010). Shortly after, the heart starts beating, and blood flow in the dorsal artery (DA)/ posterior cardinal vein (PCV) loop is observed by 28 h post fertilization (hpf ).…”

Section: Tip Cell Selection and Functionmentioning

confidence: 99%

Cell behaviors and dynamics during angiogenesis

et al. 2016

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Vascular networks are formed and maintained through a multitude of angiogenic processes, such as sprouting, anastomosis and pruning. Only recently has it become possible to study the behavior of the endothelial cells that contribute to these networks at a single-cell level in vivo. This Review summarizes what is known about endothelial cell behavior during developmental angiogenesis, focusing on the morphogenetic changes that these cells undergo.

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“…The following transgenic lines were utilized: Tg(kdrl:mCherry) ci5 (Proulx et al, 2010), Tg(kdrl:GFP) s843 (Jin et al, 2005), Tg(fli1a:nls-EGFP) y7 (Roman et al, 2002), Tg(TP1bglob:EGFP) um14 (Parsons et al, 2009) and Tg(TP1bglob:VenusPEST) S940 (Ninov et al, 2012).…”

Section: Zebrafish Experimentsmentioning

confidence: 99%

Dynamic regulation of VEGF-inducible genes by an ERK-ERG-p300 transcriptional network

et al. 2017

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The transcriptional pathways activated downstream of vascular endothelial growth factor (VEGF) signaling during angiogenesis remain incompletely characterized. By assessing the signals responsible for induction of the Notch ligand delta-like 4 (DLL4) in endothelial cells, we find that activation of the MAPK/ERK pathway mirrors the rapid and dynamic induction of DLL4 transcription and that this pathway is required for DLL4 expression. Furthermore, VEGF/ERK signaling induces phosphorylation and activation of the ETS transcription factor ERG, a prerequisite for DLL4 induction. Transcription of DLL4 coincides with dynamic ERG-dependent recruitment of the transcriptional co-activator p300. Genome-wide gene expression profiling identified a network of VEGF-responsive and ERG-dependent genes, and ERG chromatin immunoprecipitation (ChIP)-seq revealed the presence of conserved ERG-bound putative enhancer elements near these target genes. Functional experiments performed in vitro and in vivo confirm that this network of genes requires ERK, ERG and p300 activity. Finally, genome-editing and transgenic approaches demonstrate that a highly conserved ERG-bound enhancer located upstream of HLX (which encodes a transcription factor implicated in sprouting angiogenesis) is required for its VEGFmediated induction. Collectively, these findings elucidate a novel transcriptional pathway contributing to VEGF-dependent angiogenesis.

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Cranial vasculature in zebrafish forms by angioblast cluster-derived angiogenesis

Cited by 162 publications

References 41 publications

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Cell behaviors and dynamics during angiogenesis

Dynamic regulation of VEGF-inducible genes by an ERK-ERG-p300 transcriptional network

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