Crambe

Rudloff, E.; Wang, Youping

doi:10.1007/978-3-642-14871-2_5

Cited by 13 publications

(6 citation statements)

References 80 publications

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“…Crambe abyssinica (crambe) is an allohexaploid (2n = 6× = 90) with an estimated genome size of approximately 3.5 Gb based on its 2C-value (=7.04 pg) [ 1 – 5 ]. A member of the genus Crambe abyssinica distribute unevenly among four major geographical regions: Macronesian, Mediterranean, East Africa, and Euro-Siberian-southwest Asia [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

High-throughput development of simple sequence repeat markers for genetic diversity research in Crambe abyssinica

Lin

Liu

et al. 2016

BMC Plant Biol

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BackgroundThe allohexaploid Crambe abyssinica (crambe) is an oilseed crop that has been recognized for its potential value in the chemical industry, particularly in terms of producing high-erucic acid content vegetable oil. However, as an understudied crop, improvement of crambe has been hampered by the lack of genetic and genomic information to enhance its yield, oil quality and resistance against biotic and abiotic stress. Development of molecular markers is therefore of great significance to facilitate genetic improvement of crambe.ResultsIn this study, high-throughput sequencing was performed to generate sequences for the transcriptome and genome of a widely planted crambe cultivar, Galactica. A total of 186,778 expressed sequence tag (EST) contigs as 8,130,350 genomic contigs were assembled as well. Altogether, 82,523 pairs of primers were designed in the flanking sequences of the simple sequence repeat (SSR) within these contigs. Virtual PCR analysis showed that a fraction of these primers could be mapped onto the genomes of related species of Brassica, including Brassica rapa, B. oleraceae and B. napus. Genetic diversity analysis using a subset of 166 markers on 30 independent C. abyssinica accessions exhibited that 1) 95 % of the designed SSRs were polymorphic among these accessions; 2) the polymorphism information content (PIC) value of the markers ranged from 0.13 to 0.89; 3) the genetic distances (coefficient NEI72) between accessions varied from 0.06 to 0.36. Cluster analysis subsequent on the accessions demonstrated consistency with crambe breeding history. F-statistics analysis revealed a moderate level of genetic differentiation in C. abyssinica (Gst = 0.3934) and a accordingly low estimated gene flow (Nm = 0.7709).ConclusionApplication of high-throughput sequencing technology has facilitated SSR marker development, which was successfully employed in evaluating genetic diversity of C. abyssinica as demonstrated in our study. Results showed these molecular markers were robust and provided powerful tools for assessing genetic diversity and estimating crambe breeding history. Moreover, the SSR primers and sequence information developed in the study are freely available to the research community.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0828-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

High-throughput development of simple sequence repeat markers for genetic diversity research in Crambe abyssinica

Lin

Liu

et al. 2016

BMC Plant Biol

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“…There are numerous Crambe species beside C. abyssinica that have been used for food, feed or other purposes. 48–51 Out of a wide range of the species the most common ones used for food are C. maritima (Sea Kale), C. cordifolia (Giant Colewort), C. orientalis , C. tataria (Tartar Bread Plant), C. kotschyana , 52 C. aspera , C. koktebelica , C. pinnatifida and C. steveniana . 51 Crambe leaves and roots are known to have been consumed since ancient times.…”

Section: The Use Of Underestimated Wild Crambe Speciesmentioning

confidence: 99%

Biotechnological approach for improvement of Crambe species as valuable oilseed plants for industrial purposes

Pushkarova

Yemets

2022

RSC Adv.

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“…Erucic acid (omega‐9 C22:1 fatty acid) is a very important lipid compound produced by plants. Erucic acid and its derivatives, such as erucamide, behenic acid, or alcohol, have wide industrial uses as, for example, surfactants, lubricants, and pour point depressants (Rudloff & Wang, ; Vargas‐Lopez, Wiesenborn, Tostenson, & Cihacek, ). Erucic acid only occurs in the seed oil of the plant families Brassicaceae and Tropaeolaceae .…”

Section: Introductionmentioning

confidence: 99%

“…For infant formulas, EC Directive 2006/141/EC (2006) states a limit of 1% erucic acid of total fatty acids. Because of the health risk, another member of the Brassicaceae family, C. abyssinica (crambe) is considered as a candidate to substitute HEAR for high erucic acid vegetable oil production (Bruun & Matchett, ; Rudloff & Wang, ). The main advantages of crambe, with regards to the risk of erucic acid vegetable oil entering the food chain are (a) identity preservation is easy as it is morphologically very distinct from rapeseed, both as a crop and as seed; (b) it does not outcross with rapeseed as HEAR can with Canola.…”

Section: Introductionmentioning

confidence: 99%

Genetically engineering Crambe abyssinica—A potentially high‐value oil crop for salt land improvement

Tinnenbroek-Capel

Salentijn

et al. 2018

Land Degrad Dev

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Crambe abyssinica (crambe) is a new industrial oil crop that can grow on saline soil and tolerates salty water irrigation. Genetically engineered crambe in which the seed‐oil composition is manipulated for more erucic acid and less polyunsaturated fatty acid (PUFA) would be highly beneficial to industry. In this research, lysophosphatidic acid acyltransferase 2 RNA interference (CaLPAT2‐RNAi) was introduced into the crambe genome to manipulate its oil composition. The result showed in comparison with wild type, CaLPAT2‐RNAi could significantly reduce linoleic and linolenic acid content, simultaneously increasing erucic acid content. Systematic metabolism engineering was then carried out to further study CaLPAT2‐RNAi, combined with the overexpression of Brassica napus fatty acid elongase (BnFAE), Limnanthes douglasii LPAT (LdLPAT), and RNAi of endogenous fatty acid desaturase 2 (CaFAD2‐RNAi). Oil composition analysis on the tranformants' seeds showed that (a) with CaFAD2‐RNAi, PUFA content could be dramatically decreased, in comparison with BnFAE + LdLPAT + CaFAD2‐RNAi, and BnFAE + LdLPAT + CaFAD2‐RNAi + CaLPAT2‐RNAi seeds showed lower linolenic acid content; (b) BnFAE + LdLPAT + CaFAD2‐RNAi + CaLPAT2‐RNAi could increase the erucic acid content in crambe seed oil from less than 66.6% to 71.6%, whereas the highest erucic acid content of BnFAE + LdLPAT + CaFAD2‐RNAi was 79.2%; (c) although the four‐gene combination could not increase the erucic acid content of seed oil to a higher level than the others, it led to increased carbon resource deposited into C22:1 and C18:1 moieties and lower PUFA. Summarily, the present research indicates that suppression of LPAT2 is a new, promising strategy for seed‐oil biosynthesis pathway engineering, which would increase the value of crambe oil.

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Crambe

Cited by 13 publications

References 80 publications

High-throughput development of simple sequence repeat markers for genetic diversity research in Crambe abyssinica

High-throughput development of simple sequence repeat markers for genetic diversity research in Crambe abyssinica

Biotechnological approach for improvement of Crambe species as valuable oilseed plants for industrial purposes

Genetically engineering Crambe abyssinica—A potentially high‐value oil crop for salt land improvement

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