CpG-Induced Th1-Type Response in the Downmodulation of Early Development of Allergy and Inhibition of B7 Expression on T Cells of Newborn Mice

Brito, Cyro Alves de; Fusaro, Ana Elisa; Victor, Jefferson Russo; Rigato, Paula Ordonhez; Goldoni, Adriana Letícia; Muniz, Bruno Pacola; Duarte, Alberto J. S.; Sato, Maria Notomi

doi:10.1007/s10875-009-9358-9

Cited by 10 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This model may constitute a better approximation to a natural allergenic sensitization, in which allergic individuals tend to be exposed to low allergen doses, independently of the entry route, than the so far published experimental murine models, that use higher Bt E doses [[14-16] and [35]].…”

Section: Discussionmentioning

confidence: 99%

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

et al. 2010

View full text Add to dashboard Cite

BackgroundThe dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Although a mouse model for B. tropicalis extract (BtE)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal, the molecular composition of the allergen and the experimental protocol.ObjectivesThis work had two objectives. The first was to study the anti-B. tropicalis allergic responses in different mouse strains using a short-term model of respiratory allergy to BtE. This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. The second objective was to investigate whether the best responder mouse strain could be used in an experimental model of allergy employing relatively low BtE doses.MethodsGroups of mice of four different syngeneic strains were sensitized subcutaneously with 100 μg of BtE on days 0 and 7 and challenged four times intranasally, at days 8, 10, 12, and 14, with 10 μg of BtE. A/J mice, that were the best responders to BtE sensitization, were used to compare the B. tropicalis-specific asthma experimental model with the conventional experimental model of ovalbumin (OVA)-specific asthma. A/J mice were also sensitized with a lower dose of BtE.ResultsMice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. Finally, immunization with a relatively low BtE dose (10 μg per subcutaneous injection per mouse) was able to sensitize A/J mice, which were the best responders to high-dose BtE immunization, for the development of allergy-associated immune and lung inflammatory responses.ConclusionsThe described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE.

show abstract

Section: Discussionmentioning

confidence: 99%

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In addition, injection of non-allergic mothers with IFNγ on gestational day 6.5 protects against the development of allergic responses in offspring [51]. Fedolov et al [52] demonstrated that treatment of the offspring from allergic mothers on postnatal day 4 with CpG oligonucleotides, a TLR9 agonist and Th1-type stimulant [53], protected the offspring from the development of allergic responses to suboptimal OVA using the model in Fig. 1a, b.…”

Section: Maternal Exposure To Th2-inducing Environmental Irritants Almentioning

confidence: 99%

Maternal Influences over Offspring Allergic Responses

Cook‐Mills

2015

Curr Allergy Asthma Rep

View full text Add to dashboard Cite

Asthma occurs as a result of complex interactions of environmental and genetic factors. Clinical studies and animal models of asthma indicate offspring of allergic mothers have increased risk of development of allergies. Environmental factors including stress-induced corticosterone and vitamin E isoforms during pregnancy regulate the risk for offspring development of allergy. In this review, we discuss mechanisms for the development of allergic disease early in life, environmental factors that may impact the development of risk for allergic disease early in life, and how the variation in global prevalence of asthma may be explained, at least in part, by some environmental components.

show abstract

“…During this time, diverse mechanisms have been suggested, including passive transfer of maternal antibodies, maternal/fetal antigen exposure and tolerance induction. [1][2][3][4][5][6][7][8][9][10][11][12] However, immunoglobulin (Ig)G antibodies have recently been recognized as significant molecules mediating the regulation of childhood immunity.…”

Section: Introductionmentioning

confidence: 99%

Do different IgG repertoires play a role in B‐ and T‐cell functional modulation during ontogeny? The “hooks without bait” theory

Victor

2020

Immunol Cell Biol

View full text Add to dashboard Cite

The mechanisms by which immunoglobulin (Ig)G can modulate immunity have been investigated over the past few decades. In the past three years, some studies have demonstrated that IgG can play a pivotal role in mediating complex interactions that result in functional lymphocyte modulation during maturation in self or offspring primary lymphoid organs. This effect appears to be dependent on the IgG repertoire in the absence of the influence of antigens and the functionality of diverse cell populations, including B, abT (CD4 T and CD8 T), invariant natural killer T and cdT cells, in mice and humans. Based on the literature, especially on findings resulting from the therapeutic use of purified IgG (intravenous Ig) and recent pieces of evidence obtained by my group, the "hooks without bait" theory is described here to guide the future development of therapies for specific immune regulation.

show abstract

CpG-Induced Th1-Type Response in the Downmodulation of Early Development of Allergy and Inhibition of B7 Expression on T Cells of Newborn Mice

Abstract: Altogether, we verified qualitative and quantitative evidences regarding CpG effect on neonatal and adult allergens immunizations, which points to the importance of understanding neonatal immune system to establish immunomodulatory strategies for prevention of allergic diseases.

Cited by 10 publications

References 40 publications

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

Maternal Influences over Offspring Allergic Responses

Do different IgG repertoires play a role in B‐ and T‐cell functional modulation during ontogeny? The “hooks without bait” theory

Contact Info

Product

Resources

About