CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect

Anada, Takahisa; Okada, Naoko; Minari, Jusaku; Karinaga, Ryouji; Mizu, Masami; Koumoto, Kazuya; Shinkai, Seiji; Sakurai, Kazuo

doi:10.1016/j.bmcl.2005.11.070

Cited by 12 publications

(10 citation statements)

References 12 publications

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“…2004). In addition, mannan or zymosan CpG–DNA complexes recognized by TLR9 (Anada et al. 2006; Dan et al.…”

Section: Discussionmentioning

confidence: 99%

“…Our findings suggest that the immunostimulatory activity of the yeast strains examined is mediated through the interaction of TLR4 with the cell wall glucan present on the strains 630 and 982, or through TLR2, -6 heterodimer interaction with mannan present on the strains 630, 982 and KK1 (Roeder et al 2004). In addition, mannan or zymosan CpG-DNA complexes recognized by TLR9 (Anada et al 2006;Dan et al 2008) may also be responsible for the immunostimulatory activity of the 982 strain.…”

Section: Discussionmentioning

confidence: 99%

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Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa

Kourelis

Kotzamanidis

Litopoulou-Tzanetaki

et al. 2010

Journal of Applied Microbiology

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Aims: To determine the immunostimulatory activity of 15 presumptive probiotic yeast strains in the dorsal air pouch system in comparison with their activity in the gut mucosa. Methods and Results: Presumptive probiotic yeast strains previously isolated from human gastrointestinal tract and Feta cheese were further characterized genotypically and biochemically. The Saccharomyces cerevisiae 982, Saccharomyces boulardii KK1 and Kluyveromyces lactis 630 strains exhibited in the air pouch increased polymorphonuclear cell influx and phagocytic activity as well as cytokine production with similar potency as the probiotics Ultra levure S. boulardii and Lactobacillus acidophilus NCFB 1748. Oral administration of these strains in mice results in differential activation of small intestine immune responses concerning IgA and cytokine production as well as Toll‐like receptor expression. Conclusion: Besides the Saccharomyces strains 982 and KK1, the K. lactis 630 strain could also be considered as a candidate probiotic. Significance and Impact of the Study: The air pouch model may be used as an alternative and rapid method for the discrimination and selection of potential probiotic yeast strains.

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“…2004). In addition, mannan or zymosan CpG–DNA complexes recognized by TLR9 (Anada et al. 2006; Dan et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa

Kourelis

Kotzamanidis

Litopoulou-Tzanetaki

et al. 2010

Journal of Applied Microbiology

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“…The sequences for CpG ODN and its negative control were 5′-TCC ATG ACG TTC CTG ATG-A 40 -3′ and 5′-TCC ATG AGC TTC CTG ATG-A 40 -3′ (containing GpC instead of CpG), and denoted by CpGA and non-CpGA, respectively (Mizu et al, 2004c;Anada et al, 2006;Ikeda et al, 2007;Shimada et al, 2007;Minari et al, 2008). Both sequences had poly(dA) 40 tail attached at the 3′ end in order to hybridize with poly(dT).…”

Section: Materials and Dna Hybridizationmentioning

confidence: 99%

Enhanced Cytokine Secretion Owing to Multiple CpG Side Chains of DNA Duplex

2008

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Unmethylated CpG sequences (CpG ODN) stimulate Toll-like receptor 9 (TLR9) to activate innate immunity. We made DNA duplexes from poly(dT)320 and CpG ODN with (dA)40 attached at the 3' end. Circular dichroism and gel electrophoresis indicated that the CpG parts turned outward from the duplex. When we changed the CpG ODN/poly(dT) molar ratio, the amount of IL-12 secreted from J774A.1 cells (murine macrophage-like) reached the maximum at the compositions with two to four CpG portions in one duplex, while the maximum loading was eight CpG ODNs per one poly(dT)320. When the residual free dT parts were hybridized with its control GpC ODN with (dA)40 tail or just (dA)40, the maximum disappeared and the secretion increased with increasing the CpG molar ratio. These results indicated that there is a particular DNA higher-order structure to activate TLR9 more efficiently than single CpG ODN.

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“…Computational chemistry proved that the complex has an exact stoichiometric composition in which two main chain glucoses bind with one nucleotide, presumably with the combination of the hydrogen bonds and hydrophobic interactions (11). Later, it is shown that this complexation phenomena is generally observed for the β-1,3-D-glucan family including zymosan (12). that the binding stability is further enhanced by phosphorothioation of the polynucleotide backbone of poly(dA), denoted by S-dA x (13,14).…”

Section: A Introductionmentioning

confidence: 96%

Application toward Drug Delivery System Using ^|^beta;-1,3-Glucan

Maegawa

Mochizuki

Miyamoto

et al. 2015

TIGG

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A β-1,3-D-glucan called Schizophyllan (SPG) can form a novel complex with homo oligodeoxynucleotides (ODNs) via the combination of hydrogen bonding and hydrophobic interactions. Dectin-1 is a major receptor involved in the recognition of β-1,3-D-glucans and expressed on antigen presenting cells (APCs) including macrophages, dendritic cells, monocytes, neutrophils, and a subset of T cells. Therefore, the SPG/ODN complex can be used as APCs cell-specific delivery of antisense ODNs (AS-ODNs) or unmethylated CpG sequences (CpG-ODNs). In fact, AS-ODN/SPG complex protected mice from LPS-induced hepatitis and CpG-ODN/SPG complex induced high antibody titers when it was administered to cynomolgus monkeys as adjutant of influenza vaccine. These results indicate that SPG can be an excellent drug delivery system carrier targeting APCs.

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CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect

Cited by 12 publications

References 12 publications

Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa

Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa

Enhanced Cytokine Secretion Owing to Multiple CpG Side Chains of DNA Duplex

Application toward Drug Delivery System Using ^|^beta;-1,3-Glucan

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