COVID-19: molecular diagnostics overview

Touma, Marlin

doi:10.1007/s00109-020-01931-w

Cited by 52 publications

(39 citation statements)

References 54 publications

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“…This can be attained by sensitive and specific diagnostic tools such as gene sequencing, electron microscopy, and cell culture methods [7] . Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) has been the most common rapid diagnostic test in diagnosing SARS-CoV-2, as it couples the principle of transcription and amplification, thereby providing a high specificity rate [8] . Specimens for virus detection vary from nasopharyngeal and oropharyngeal swabs and broncho-alveolar lavage, sputum to stool, urine, and blood, depending on the clinical manifestation of the patients [9] .…”

Section: Resultsmentioning

confidence: 99%

A rapid review of recent advances in diagnosis, treatment and vaccination for COVID-19

Umakanthan

Chattu

Ranade³

et al. 2021

AIMS Public Health

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COVID-19 is caused by SARS-CoV-2, which originated in Wuhan, Hubei province, Central China, in December 2019 and since then has spread rapidly, resulting in a severe pandemic. The infected patient presents with varying non-specific symptoms requiring an accurate and rapid diagnostic tool to detect SARS-CoV-2. This is followed by effective patient isolation and early treatment initiation ranging from supportive therapy to specific drugs such as corticosteroids, antiviral agents, antibiotics, and the recently introduced convalescent plasma. The development of an efficient vaccine has been an on-going challenge by various nations and research companies. A literature search was conducted in early December 2020 in all the major databases such as Medline/PubMed, Web of Science, Scopus and Google Scholar search engines. The findings are discussed in three main thematic areas namely diagnostic approaches, therapeutic options, and potential vaccines in various phases of development. Therefore, an effective and economical vaccine remains the only retort to combat COVID-19 successfully to save millions of lives during this pandemic. However, there is a great scope for further research in discovering cost-effective and safer therapeutics, vaccines and strategies to ensure equitable access to COVID-19 prevention and treatment services.

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Section: Resultsmentioning

confidence: 99%

A rapid review of recent advances in diagnosis, treatment and vaccination for COVID-19

Umakanthan

Chattu

Ranade³

et al. 2021

AIMS Public Health

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“…These assays are expected to be simple, fast, accurate, safe, and amenable to be used in local hospital and clinic settings bearing the burden of testing and treating patients [47]. Recent studies evaluated different tests available and explored the possibility of improving SARS-CoV-2 diagnosis [47][48][49]. Tests based on biomarkers (e.g., serum porphobilinogen and aminolaevulinic acid) can be sensitive, specific, and low cost, and could even be used to monitor the response to treatments [50].…”

Section: Variations Of Pcr Techniques and Diagnosis Methodsmentioning

confidence: 99%

Diagnosing the novel SARS-CoV-2 by quantitative RT-PCR: variations and opportunities

et al. 2020

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The world is currently facing a novel viral pandemic (SARS-CoV-2), and large-scale testing is central to decision-making for the design of effective policies and control strategies to minimize its impact on the global population. However, testing for the presence of the virus is a major bottleneck in tracking the spreading of the disease. Given its adaptability regarding the nucleotide sequence of target regions, RT-qPCR is a strong ally to reveal the rapid geographical spreading of novel viruses. We assessed PCR variations in the SARS-CoV-2 diagnosis taking into account public genome sequences and diagnosis kits used by different countries. We analyzed 226 SARS-CoV-2 genome sequences from samples collected by March 22, 2020. Our work utilizes a phylogenetic approach that reveals the early evolution of the virus sequence as it spreads around the globe and informs the design of RT-qPCR primers and probes. The quick expansion of testing capabilities of a country during a pandemic is largely impaired by the availability of adequately trained personnel on RNA isolation and PCR analysis, as well as the availability of hardware (thermocyclers). We propose that rapid capacity development can circumvent these bottlenecks by training medical and nonmedical personnel with some laboratory experience, such as biology-related graduate students. Furthermore, the use of thermocyclers available in academic and commercial labs can be promptly calibrated and certified to properly conduct testing during a pandemic. A decentralized, fast-acting training and testing certification pipeline will better prepare us to manage future pandemics.

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“…IL-6, çok sayıda hücrenin ve dokunun üzerinde birçok farklı etkiye sahip olan bir sitokindir. Bu sitokin aynı zamanda termoregülasyonda rol oynayan akut faz proteinlerinin üretimini uyararak ciddi doku hasarı ve birçok organda işlev bozukluğuna yol açabilmektedir (17). Aynı zamanda IL-6 dışında Covid-19 tanısı alan hastaların plazma örneklerinde yüksek miktarda lökosit sayıları, anormal solunum ve yüksek seviyede pro-inflamatuvar sitokin üretimi gözlemlenmiştir.…”

Section: Koronavirüs Ailesi Ve Covid-19unclassified

Covid-19 ve Kanser Covid-19 and Cance

Karataş¹,

Tunçdemi̇r²

2018

TFK

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Öz2019 Aralık ayında Çin'in Wuhan şehrinde ortaya çıkan ve koronavirüs ailesinin yeni üyesi olan SARS-CoV-2 kısa sürede tüm dünyaya yayılmış ve pandemi ilan edilmesine neden olmuş bir virüstür. Covid-19 olarak adlandırılan bu hastalık kronik rahatsızlığı bulunan ve yaşı 65 üzeri olan bireylerde ölüm riskini arttırmaktadır. Covid-19'un patogenezinde ölümü tetikleyen en ciddi mekanizmaların başında oluşturduğu sitokin fırtınası gelmektedir. Günümüzde hastalığa karşı aşı veya bu mekanizmaları düzenleyecek bir ilaç araştırmaları devam etse de ne yazık ki henüz net bir tedavi yöntemi bulunmamaktadır. Hızlı yayılması ve vaka sayılarındaki artış sağlık sektöründe büyük bir yoğunluğa neden olduğu için Covid-19 tanısı konmayan fakat kanser gibi ciddi rahatsızlıkları olan bireylerin enfekte olma riskini azaltmaya yönelik kılavuzlar yayınlanmıştır. Bu derlemede Covid-19'un kökenini, patolojisini, neden mortalitesinin belirli durumlarda yüksek olduğunun yanı sıra bu hastalığın kanser hastalarına olan etkisi, Covid-19 tanısı konulan kanser hastalarının tedavisindeki komplikasyonlar ve kanser tedavisinde kullanılan inhibitörlerin Covid-19 üzerine etkisini kapsayan tedavi biçimlerini içeren kılavuz ve çalışmalar tartışılacaktır.

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COVID-19: molecular diagnostics overview

Cited by 52 publications

References 54 publications

A rapid review of recent advances in diagnosis, treatment and vaccination for COVID-19

A rapid review of recent advances in diagnosis, treatment and vaccination for COVID-19

Diagnosing the novel SARS-CoV-2 by quantitative RT-PCR: variations and opportunities

Covid-19 ve Kanser Covid-19 and Cance

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