Covalently Fluorophore-Functionalized ZIF-8 Colloidal Particles as a Sensing Platform for Endocrine-Disrupting Chemicals Such as Phthalates Plasticizers

Chapartegui-Arias, Ander; Villajos, José A.; Myxa, Anett; Beyer, Sven; Falkenhagen, Jana; Schneider, R.; Emmerling, Franziska

doi:10.1021/acsomega.9b01051

Cited by 13 publications

(17 citation statements)

References 34 publications

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“…The authors have previously published, for the first time, the chemical analysis of frameworks by HPLC. [15,20] Notably, the final composition of Z8P by HPLC is consistent with 2-mim being included on the composition of the hybrid fluorescent ZIF, as required to achieve the desired nanostructure. HPLC analysis supports that most of the imidazolate moieties in Z8P are i2ca which are those that include the aldehyde functionality.…”

Section: Resultsmentioning

confidence: 79%

“…A response comparable to the one reported previously by us for this system was recorded (Figure S10 in the Supporting Information). [15] To turn the enzyme immunoassay into a fluorescence immunoassay based on the inherent fluorescent properties of Z8P, the model non-competitive indirect ELISA was performed at higher concentrations (Sections 7.4.1 and 7.4.2 in the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

“…We had recently published the synthesis of a luminescent nano ZIF covalently linked to a fluorescent pyrene, referred as Z8P, for the sensitive detection of phthalate plasticizers which can act as endocrine‐disrupting chemicals [15] . The fluorescent nano Z8P has the pyrene fluorophore covalently linked via an imine bond.…”

Section: Introductionmentioning

confidence: 99%

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Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate**

Chapartegui-Arias

Raysyan

Belenguer

et al. 2021

Chemistry A European J

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Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the conjugates and the possibility to tailor their mobility gives a huge potential for diagnostic assays. An enzyme-linked immuno-sorbent assay (ELISA) with horseradish peroxidase (HRP) as label, proved the integrity, stability, and dispersibility of the antibody conjugates, LC-MS/MS provided evidence that a covalent link was established between these metal-organic frameworks and lysine residues in IgG antibodies.

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Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

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Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate**

Chapartegui-Arias

Raysyan

Belenguer

et al. 2021

Chemistry A European J

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“…An experiment was designed using the analytical conditions developed in previous work. [10] to assess the changes in fluorescence quenching of the conjugate, Z8P-IgG, when exposed to a model contaminant, dimethyl phthalate, DMP.…”

Section: Fluorescence Characterization the Conjugated Z8p Particles Vmentioning

confidence: 99%

Zeolitic Imidazolate Framework Antibody Conjugates in Bioanalytical Applications

Arias¹,

Raysyan²,

Belenguer³

et al. 2020

Preprint

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<p>Improvements in bioanalytical technologies are driven by evolving threats and challenges to the global community. These challenges include emerging diseases, the need for substance screening and increasing environmental pollution. Here, we present a new bioanalytical concept that employs nanosized Metal-Organic Framework (MOF) particles as labels for antibody-based analytical methods, including enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA). A strategy for covalently linking antibodies to form stable, colloidal Zeolitic Imidazolate Frameworks (ZIFs) conjugates is described. The proof of principle for their use in LFIA is presented. In particular, ZIF-antibody conjugates displayed excellent behaviour in dispersion and good mobility via lateral flow on a solid substrate while retaining full selectivity of the antibody. Moreover, protein aggregation of the antibodies was prevented, suggesting protection against degradation in an exoskeleton-like manner. Our study could inspire future work to address global bioanalytical and diagnostic challenges.</p>

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“…This accurate control makes MOFs predestined for drug loading and sustained or triggered release from nano-particulate drug formulations or coatings ( Wang et al, 2020 ). This has led to the classical understanding of “biomedical MOF applications” that primarily focused on diagnostic applications ( Chapartegui-Arias et al, 2019 ) or drug delivery ( Keskin and Kizilel, 2011 ; Banerjee et al, 2020 ). The present review is clearly delimited from these established fields of investigation by focusing on MOFs for TERM applications.…”

Section: Introductionmentioning

confidence: 99%

Metal-Organic Framework (MOF)-Based Biomaterials for Tissue Engineering and Regenerative Medicine

Shyngys

Ren

Xiao-qi

et al. 2021

Front. Bioeng. Biotechnol.

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The synthesis of Metal-organic Frameworks (MOFs) and their evaluation for various applications is one of the largest research areas within materials sciences and chemistry. Here, the use of MOFs in biomaterials and implants is summarized as narrative review addressing primarely the Tissue Engineering and Regenerative Medicine (TERM) community. Focus is given on MOFs as bioactive component to aid tissue engineering and to augment clinically established or future therapies in regenerative medicine. A summary of synthesis methods suitable for TERM laboratories and key properties of MOFs relevant to biomaterials is provided. The use of MOFs is categorized according to their targeted organ (bone, cardio-vascular, skin and nervous tissue) and whether the MOFs are used as intrinsically bioactive material or as drug delivery vehicle. Further distinction between in vitro and in vivo studies provides a clear assessment of literature on the current progress of MOF based biomaterials. Although the present review is narrative in nature, systematic literature analysis has been performed, allowing a concise overview of this emerging research direction till the point of writing. While a number of excellent studies have been published, future studies will need to clearly highlight the safety and added value of MOFs compared to established materials for clinical TERM applications. The scope of the present review is clearly delimited from the general ‘biomedical application’ of MOFs that focuses mainly on drug delivery or diagnostic applications not involving aspects of tissue healing or better implant integration.

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Covalently Fluorophore-Functionalized ZIF-8 Colloidal Particles as a Sensing Platform for Endocrine-Disrupting Chemicals Such as Phthalates Plasticizers

Cited by 13 publications

References 34 publications

Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate**

Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate**

Zeolitic Imidazolate Framework Antibody Conjugates in Bioanalytical Applications

Metal-Organic Framework (MOF)-Based Biomaterials for Tissue Engineering and Regenerative Medicine

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