Covalent structure of three phase-1 flagellar filament proteins of Salmonella

Li, Wei; Joys, Terence M.

doi:10.1016/0022-2836(85)90397-3

Cited by 149 publications

(149 citation statements)

References 17 publications

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“…All sequences were highly conserved at the extreme 5Ј and 3Ј ends and diverged, with respect to other alleles, as they approached the middle, variable portion of the gene. Conserved and variable regions were consistent with previous designations (15,29).…”

Section: Resultssupporting

confidence: 86%

“…1). Three of the four groups contained multiple antigenic types and were designated the G complex, the Z4 complex, and the ␣ cluster; the fourth group contained a single antigenic type, z 29 . The G complex contained all alleles encoding flagellar antigen g plus alleles encoding the immunologically related antigen m,t.…”

Section: Vol 42 2004 Analysis Of Flagellin Genes Of Salmonella 1927mentioning

confidence: 99%

“…Flagellar antigens z 36 ; z 38 ; and z 36 ,z 38 had not previously been noted to be related to the Z4 complex. The ␣ cluster contained the remainder of the flagellar antigen types, with the exception of H:z 29 , which separated into a group by itself.…”

Section: Vol 42 2004 Analysis Of Flagellin Genes Of Salmonella 1927mentioning

confidence: 99%

“…Alleles in the four clusters exhibited different levels of diversity within the variable region depending on the cluster. H:z 29 subspecies had greater than 98% amino acid identity within the variable region. With the exception of the most divergent antigens, H:m,t and H:g,z 51 , alleles in the G complex had at least 90% amino acid identity within the variable region; some antigens, such as H:g,p and H:g,p,s, differed by a single amino acid.…”

Section: Vol 42 2004 Analysis Of Flagellin Genes Of Salmonella 1927mentioning

confidence: 99%

“…The conserved regions encode the flagellar filament backbone and are critical for the assembly of the filament. The central region, corresponding approximately to amino acids 181 to 390, encodes the surface-exposed and antigenically variable portion of the filament (13)(14)(15)29). Several studies have reported DNA sequences for Salmonella flagellin genes (6,11,13,15,17,24,25,27,29).…”

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confidence: 99%

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Sequencing and Comparative Analysis of Flagellin Genes fliC , fljB , and flpA from Salmonella

et al. 2004

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Salmonella isolates have traditionally been classified by serotyping, the serologic identification of two surface antigens, O-polysaccharide and flagellin protein. Serotyping has been of great value in understanding the epidemiology of Salmonella and investigating disease outbreaks; however, production and quality control of the hundreds of antisera required for serotyping is difficult and time-consuming. To circumvent the problems associated with antiserum production, we began the development of a system for determination of serotype in Salmonella based on DNA markers. To identify flagellar antigen-specific sequences, we sequenced 280 alleles of the three genes that are known to encode flagellin in Salmonella, fliC, fljB, and flpA, representing 67 flagellar antigen types. Analysis of the data indicated that the sequences from fliC, fljB, and flpA clustered by the antigen(s) they encode not by locus. The sequences grouped into four clusters based on their conserved regions. Three of the four clusters included multiple flagellar antigen types and were designated the G complex, the Z4 complex, and the ␣ cluster. The fourth cluster contained a single antigen type, H:z 29 . The amino acid sequences of the conserved regions within each cluster have greater than 95% amino acid identity, whereas the conserved regions differ substantially between clusters (75 to 85% identity). Substantial sequence heterogeneity existed between alleles encoding different flagellar antigens while alleles encoding the same flagellar antigen were homologous, suggesting that flagellin genes may be useful targets for the molecular determination of flagellar antigen type.

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Section: Resultssupporting

confidence: 86%

Section: Vol 42 2004 Analysis Of Flagellin Genes Of Salmonella 1927mentioning

confidence: 99%

Section: Vol 42 2004 Analysis Of Flagellin Genes Of Salmonella 1927mentioning

confidence: 99%

Section: Vol 42 2004 Analysis Of Flagellin Genes Of Salmonella 1927mentioning

confidence: 99%

mentioning

confidence: 99%

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Sequencing and Comparative Analysis of Flagellin Genes fliC , fljB , and flpA from Salmonella

et al. 2004

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Salmonella

Threlfall¹

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Intragenic recombination in a flagellin gene: characterization of the H1-j gene of Salmonella typhi.

et al. 1989

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Salmonella typhi, the etiologic agent of typhoid fever, typically has only a phase-1 flageliar antigen, d, but some isolates, found only in Indonesia, have antigenj instead, and may have a second flageliar antigen, z66. It appears that intragenic recombination involving a directly repeated 11 bp sequence in the HJ-d flagellin gene changed the flagellar antigen toj, by deleting 261 bp in its central, antigenically determinant, part. Sequencing of the hypervariable regions of genes HJ-d and Hi-j, and hybridization of such genes, after amplification by the polymerase chain reaction, with oligonucleotide probes specific for the deleted segment or for the sequence produced by the recombination confirmed that all thej alleles have the postulated deletion. By applying the polymerase chain reaction to study S.typhi isolates from Jakarta, not previously tested in respect to flageliar antigen, we showed that gene Hi-j was nearly as common as HJ-d in these isolates.

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Covalent structure of three phase-1 flagellar filament proteins of Salmonella

Cited by 149 publications

References 17 publications

Sequencing and Comparative Analysis of Flagellin Genes fliC , fljB , and flpA from Salmonella

Sequencing and Comparative Analysis of Flagellin Genes fliC , fljB , and flpA from Salmonella

Salmonella

Intragenic recombination in a flagellin gene: characterization of the H1-j gene of Salmonella typhi.

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