Covalent organic frameworks as crystalline sponges for enzyme extraction and production from natural biosystems

Chen, Haixin; Jin, Chaonan; Chen, Xuepeng; Yu, Jiangyue; Yan, Dong; Cheng, Peng; Zhang, Zhenjie; Zhao, Limin; Chen, Yao

doi:10.1016/j.cej.2022.136624

Cited by 5 publications

(10 citation statements)

References 35 publications

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“…In the past two decades, well-defined architectures of MOFs, [9][10][11][12] COFs, [13][14][15] metallasupramolecules, [16][17][18] organic supramolecules [19][20][21] and SOFs [22][23][24][25] have received greater attention due to their rich and designable structures, modifiable pore space and tunable functionality. 26 These architectures provide the best models for the structure-property relationship study, and the well-organized skeletons commonly possess special functions for olefin separation in hierarchical porous MOFs 27,28 for enzyme separation in mesoporous COFs, 29,30 for unusual reactions in metallasupramolecules which act as molecular flasks, 31 for photodynamic therapy efficacy in organic supramolecules 32 and for accessible film production in SOFs. 33 Like building skyscrapers, the molecular architectures can be precisely constructed by the judicious choice of divergent building blocks.…”

Section: Ai-juan LImentioning

confidence: 99%

Polypyridyl Ru(ii) or cyclometalated Ir(iii) functionalized architectures for photocatalysis

Liu

Huang

et al. 2023

Chem. Soc. Rev.

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Section: Ai-juan LImentioning

confidence: 99%

Polypyridyl Ru(ii) or cyclometalated Ir(iii) functionalized architectures for photocatalysis

Liu

Huang

et al. 2023

Chem. Soc. Rev.

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“…For example, high-purity lysozyme was successfully extracted from egg white through the adsorption and elution behavior of COFs. 69 Cationic COFs were used as solid anion exchange materials to efficiently adsorb and separate proteins and amino acids. 36 In addition, much progress has also been made in constructing large pore COFs, making more COFs beneficial for separating organic/inorganic macromolecules and biomolecules.…”

Section: Bioseparationmentioning

confidence: 99%

“…In recent years, COFs have also attracted a great deal of attention in enriching and separating various biomolecules. For example, high-purity lysozyme was successfully extracted from egg white through the adsorption and elution behavior of COFs . Cationic COFs were used as solid anion exchange materials to efficiently adsorb and separate proteins and amino acids .…”

Section: Applications Of Cofs In Biocatalysis and Bioseparationmentioning

confidence: 99%

Nanoplatforms Based on Covalent Organic Frameworks (COFs) for Biomedical Applications

Liang,

Li,

et al. 2023

Chem. Mater.

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Covalent organic frameworks (COFs) make up an emerging class of crystalline porous materials mainly composed of light elements in the form of dynamic covalent bonds. Owing to their two- or three-dimensional network structures and ideal properties, including low density, large specific surface area, high chemical stability, and good biocompatibility, COFs have shown a wide range of applications in optoelectronic devices, energy conversion and storage, adsorption and separation, sensing, organic catalysis, and biomedicine. This review provides an overview of the recent advances in functional COF-based nanoplatforms for biological diagnosis and treatment, such as enzyme catalysis, protein separation, drug delivery, photodynamic/photothermal therapy, and synergistic treatment. Challenges and future directions of developing COFs for biomedicine and related applications are also discussed. We envisage that this review will inspire materials scientists, chemists, biologists, and clinical doctors working in related fields to work closely to move this field toward clinical trials and human healthcare.

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“…Lysozyme (3.0 × 3.0 × 4.5 nm) can hydrolyze the β-(1,4)-glycosidic bond between N -acetylmuramic acid and N -acetylglucosamine residues, thus producing the bactericidal effect . To accommodate lysozyme, we designed and synthesized COFs that match its size (Figure a) . TPB-DMTP-COF, PI-COF-2, and TDA-TMT-COF all possess pore sizes of around 3.3 nm, enabling the effective immobilization of lysozyme.…”

Section: Design and Functionality Of Enzyme-immobilization Carriersmentioning

confidence: 99%

Section: Design and Functionality Of Enzyme-immobilization Carriersmentioning

confidence: 99%

Integration of Enzyme and Covalent Organic Frameworks: From Rational Design to Applications

Qiao,

Jin,

Zuo

et al. 2023

Acc. Chem. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Manufacturing is undergoing profound transformations, among which green biomanufacturing with low energy consumption, high efficiency, and sustainability is becoming one of the major trends. However, enzymes, as the "core chip" of biomanufacturing, are often handicapped in their application by their high cost, low operational stability, and nonreusability. Immobilization of enzymes is a technology that binds or restricts enzymes in a certain area with solid materials, allows them to still carry out their unique catalytic reaction, and allows them to be recycled and reused. Compared with free enzymes, immobilized enzymes boast numerous advantages such as enhanced storage stability, ease of separation, reusability, and controlled operation. Currently, commonly used supports for enzyme immobilization (e.g., mesoporous silica, sol−gel hydrogels, and porous polymer) can effectively improve enzyme stability and reduce product inhibition. However, they still face drawbacks such as potential leaching or conformational change during immobilization and poor machining performance. Especially, most enzyme carrier solid materials possess disordered structures, inevitably introducing deficiencies such as low loading capacity, hindered mass transfer, and unclear structure−property relationships. Additionally, it remains a notable challenge to meticulously design immobilization systems tailored to the specific characteristics of enzyme/reaction. Therefore, there is a significant demand for reliable solid materials to overcome the above challenges. Crystalline porous materials, particularly covalent organic frameworks (COFs), have garnered significant interest as a promising platform for immobilizing enzymes due to their unique properties, such as their crystalline nature, high porosity, accessible active sites, versatile synthetic conditions, and tunable structure. COFs create a stabilizing microenvironment that protects enzymes from denaturation and significantly enhances reusability. Nevertheless, some challenges still remain, including difficulties in loading large enzymes, reduced enzyme activities, and the limited functionality of carriers. Therefore, it is essential to develop innovative carriers and novel strategies to broaden the methods of immobilizing enzymes, enabling their application across a more diverse array of fields.The integration of enzymes with advanced porous materials for intensified performance and diverse applications is still in its infancy, and our group has done a series of pioneering works. This Account presents a comprehensive overview of recent research progress made by our group, including (i) the development of innovative enzyme immobilization strategies utilizing COFs to make the assembly and integration of enzymes and carriers more effective; (ii) rational design and construction of functional carriers for enzyme immobilization using COFs; and (iii) extensions of immobilized enzyme applications based on COFs from industrial catalysis to biomedic...

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Covalent organic frameworks as crystalline sponges for enzyme extraction and production from natural biosystems

Cited by 5 publications

References 35 publications

Polypyridyl Ru(ii) or cyclometalated Ir(iii) functionalized architectures for photocatalysis

Polypyridyl Ru(ii) or cyclometalated Ir(iii) functionalized architectures for photocatalysis

Nanoplatforms Based on Covalent Organic Frameworks (COFs) for Biomedical Applications

Integration of Enzyme and Covalent Organic Frameworks: From Rational Design to Applications

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